scholarly journals Direct quantitative determination of ceramide glycosylation in vivo: a new approach to evaluate cellular enzyme activity of glucosylceramide synthase

2009 ◽  
Vol 51 (4) ◽  
pp. 866-874 ◽  
Author(s):  
Vineet Gupta ◽  
Gauri A. Patwardhan ◽  
Qian-Jin Zhang ◽  
Myles C. Cabot ◽  
S. Michal Jazwinski ◽  
...  
1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.


2012 ◽  
Vol 81 (3) ◽  
pp. 498-505 ◽  
Author(s):  
E. Allard-Vannier ◽  
S. Cohen-Jonathan ◽  
J. Gautier ◽  
K. Hervé-Aubert ◽  
E. Munnier ◽  
...  

1974 ◽  
Vol 86 (2) ◽  
pp. 373-396 ◽  
Author(s):  
J.M. Coffin ◽  
J.T. Parsons ◽  
L. Rymo ◽  
R.K. Haroz ◽  
C. Weissmann

1995 ◽  
Vol 302 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Tsai-yun Lee ◽  
Mikio Tsuzuki ◽  
Toshifumi Takeuchi ◽  
Kenji Yokoyama ◽  
Isao Karube

1959 ◽  
Vol 52 (5) ◽  
pp. 337-346 ◽  
Author(s):  
Paul Edholm ◽  
Bertil Jacobson

1985 ◽  
Vol 49 (2) ◽  
pp. 424-427 ◽  
Author(s):  
A Morris Hooke ◽  
D O Sordelli ◽  
M C Cerquetti ◽  
A J Vogt

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