Regulation of carotenoid metabolism in response to different temperatures in citrus juice sacs in vitro

2018 ◽  
Vol 238 ◽  
pp. 384-390 ◽  
Author(s):  
Witchulada Yungyuen ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Misato Futamura ◽  
Makoto Tabuchi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Metabolism ◽  
Different Temperatures ◽  
Juice Sacs

Regulation of ascorbic acid metabolism in response to different temperatures in citrus juice sacs in vitro

2017 ◽  
Vol 217 ◽  
pp. 1-7 ◽  
Author(s):  
Witchulada Yungyuen ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Kazuki Yamawaki ◽  
Masaki Yahata ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Citrus Juice ◽  
Different Temperatures ◽  
Juice Sacs

In vitro sugar uptake by grapefruit (Citrus paradisi) juice-sac cells

2005 ◽  
Vol 32 (4) ◽  
pp. 357 ◽  
Author(s):  
Moshe Huberman ◽  
Uri Zehavi ◽  
Wilfred D. Stein ◽  
Ed Etxeberria ◽  
Raphael Goren
Keyword(s):  
Metabolic Inhibitors ◽  
Sugar Uptake ◽  
Citrus Paradisi ◽  
Citrus Juice ◽  
14Co2 Evolution ◽  
Independent Process ◽  
Initial Uptake ◽  
Juice Sacs ◽  
Slight Inhibition

To further our understanding of the mechanisms of sugar uptake and accumulation into grapefruit (Citrus paradisi Macf. cv. Marsh seedless), the patterns of uptake and utilisation of sucrose, glucose and fructose by Citrus juice cells was investigated. Analyses were conducted on sliced juice sacs that were incubated in radioactive [14C]-sugar solutions with unlabelled sugars, in the presence or absence of metabolic inhibitors. Both hexoses demonstrated an initial uptake peak in December and a second uptake peak in February–March. From March through April the rates of sucrose uptake increased to levels comparable to those of glucose and fructose. Sucrose and its moieties fructose and glucose entered the juice cells of Citrus juice fruit by an insaturable, and mostly by an independent, process. However, NaN3 and carbonylcyanide m-chlorophenylhydrazone (CCCP) produced slight inhibition of these processes. Cells took up hexoses at a greater rate than sucrose, with accumulation reaching a plateau by 4–8 h, and then continuing unabated, in the case of glucose, for 42 h. Uptake of all three sugars increased linearly in the range of sugar concentrations tested, which extended from 0.01 to 320 mm, denoting an insaturable system for sugar uptake. 14CO2 evolution was relatively low in all the experiments, the lowest evolution being recorded when the uptake of [14C]-sucrose was studied, while the highest 14CO2 evolution was recorded when the uptake of [14C]-glucose was studied. The data demonstrate a preferential utilisation of glucose over fructose and sucrose. In all the experiments, the two metabolic inhibitors significantly inhibited the decarboxylation of the three sugars.

Fairy Chemicals, 2-Azahypoxanthine and 2-Aza-8-oxohypoxanthine, Regulate Carotenoid Accumulation in Citrus Juice Sacs in Vitro

2015 ◽  
Vol 63 (32) ◽  
pp. 7230-7235 ◽  
Author(s):  
Gang Ma ◽  
Lancui Zhang ◽  
Kazuki Yamawaki ◽  
Masaki Yahata ◽  
Jae-Hoon Choi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Accumulation ◽  
Juice Sacs

scholarly journals Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro

2011 ◽  
Vol 63 (2) ◽  
pp. 871-886 ◽  
Author(s):  
Lancui Zhang ◽  
Gang Ma ◽  
Masaya Kato ◽  
Kazuki Yamawaki ◽  
Toshihiko Takagi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Accumulation ◽  
Metabolic Genes ◽  
Juice Sacs

scholarly journals Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aiyan Guan ◽  
Inge Van Damme ◽  
Frank Devlieghere ◽  
Sarah Gabriël
Keyword(s):  
Enzymatic Digestion ◽  
Infected Fish ◽  
Effect Of Temperature ◽  
Video Recordings ◽  
Different Temperatures ◽  
Semi Solid ◽  
Zoonotic Parasites ◽  
The Impact ◽  
Phosphate Buffered Saline

AbstractAnisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.

scholarly journals A novel ceramic material with medical application

2008 ◽  
Vol 2 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Joanna Podporska ◽  
Marta Błażewicz ◽  
Barbara Trybalska ◽  
Łukasz Zych
Keyword(s):  
Body Fluid ◽  
Crystallization Process ◽  
Sol Gel ◽  
Medical Application ◽  
In Vitro Test ◽  
Glass Crystallization ◽  
Complex Shapes ◽  
Different Temperatures ◽  
Vitro Test

Until now the basic methods used in manufacturing of wollastonite have been chemical (melting together with glass crystallization process, chemical coprecipitation) and sol - gel methods. A new and promising way of wollastonite fabrication is controlled pyrolysis of polysiloxane precursors with inorganic fillers. Heat treatment of such mixtures leads to the formation of wollastonite-containing ceramics already at about 1000?C. This is a relatively inexpensive and efficient method which enables to obtain complex shapes of the samples. The aim of this work was to obtain sintered, wollastonite-containing bioceramics and determine its bioactive features. Samples were sintered at three different temperatures: 1000, 1100 and 1200?C. Then the bioactivity of the wollastonite-containing ceramics was investigated by the ?in vitro? test in simulated body fluid. On the basis of the achieved results, it can be assumed that the obtained material possesses bioactive features.

scholarly journals Kefiran Ekstraktının Bazı Bitki Patojeni Bakterilerine Karşı Antimikrobiyal Etkisinin Değerlendirilmesi

2020 ◽  
Vol 8 (4) ◽  
pp. 889-894
Author(s):  
Bilgin Taşkın
Keyword(s):  
Antibacterial Effect ◽  
Antimicrobial Effect ◽  
Acetic Acid Bacteria ◽  
Fermented Milk ◽  
Diffusion Method ◽  
Milk Product ◽  
Bacterial Strains ◽  
Private Households ◽  
Different Temperatures

Kefir; is a fermented milk product which is produced by granules containing a wide variety of microorganisms such as lactic acid bacteria, acetic acid bacteria and yeasts. It is traditionally consumed in many countries. It has been shown in many studies that the polysaccharide structure surrounding the granules which is composed mainly of kefiran molecule has antimicrobial effect against various pathogens as well as many health promoting effects. In this study, 24 h fermented kefir was used with two types of kefir granules for production of kefiran extract. One of them is being sold commercially and the other was collected from private households in a different region of Turkey. Kefiran extraction was carried out from matured kefir granules using three different temperatures, 80°C, 90°C and 100°C. Also, the protein contents of the extracted solutions were determined by Bradford method. Protein content of the extract solutions obtained were measured as 0.001 g/ml. The antibacterial effect of 0.05, 0.1, 1 and 2 mg of this extract against several plant pathogenic bacterial strains belonging to genus Pseudomonas, Xanthomonas, Erwinia ve Clavibacter was investigated in vitro for the first time. For this purpose, two methods, disc diffusion method and spreading method were used. The AN and SD kefir supernatants used as the positive controls in the experiments showed an average of 13-17 mm and 10-14 mm inhibition zones on the isolates, respectively, but the antibacterial effect of kefiran extracts was not observed.

scholarly journals Analysis of Radiofrequency Lesions in Egg Whites in Vitro Produced by Application of the Tew Electrode for Different Temperatures and Times

2015 ◽  
Vol 20 (6) ◽  
pp. 316-320 ◽  
Author(s):  
Young Suk Kwon ◽  
So Young Lim ◽  
Jong Ho Kim ◽  
Ji Su Jang ◽  
Chul Ho Kim ◽  
...  
Keyword(s):  
Distal Radius ◽  
Mixed Model ◽  
Glass Container ◽  
Lesion Size ◽  
External Radius ◽  
Internal Radius ◽  
S 100 ◽  
Different Temperatures ◽  
Radiofrequency Lesions

BACKGROUND: Understanding the size and shape of radiofrequency lesions is important to reduce side effects when applied to patients.OBJECTIVES: To investigate the radiofrequency lesions produced by the application of the Tew electrode for different temperatures and times.METHODS: The white from a fresh hen’s egg was placed in a rectangular glass container and warmed to 37°C. After immersion of the Tew electrode in the egg white, radiofrequency lesions were produced at 65°C, 70°C, 75°C, 80°C, 85°C and 90°C. For each temperature, photographs were taken at 10 s, 20 s, 30 s, 40 s, 50 s, 60 s, 70 s, 80 s, 90 s, 100 s, 110 s and 120 s. The size of the lesion was measured at each temperature and time. A mixed model was used to analyze the data.RESULTS: The size of the lesion increased with increasing temperature and time. There were statistically significant differences in the size of the internal radius between the 65°C and 70°C groups and the 70°C and 75°C groups, as well as in the 70°C and 75°C groups in the size of the external radius and the 60°C to 80°C groups in the size of the distal radius. The maximum lesion size was produced at 90°C and 120 s, and was 1.06±0.16 mm in internal radius, 0.37±0.15 mm in external radius, 0.39±0.04 mm in distal radius.CONCLUSION: The Tew electrode produces lesions following the contour of the tip, and the internal radius is larger than the external and distal radius. The best combination of temperature and time for lesioning using the Tew electrode is 80°C, for 60 s to 90 s.

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