Regulation of ascorbic acid metabolism in response to different temperatures in citrus juice sacs in vitro

2017 ◽  
Vol 217 ◽  
pp. 1-7 ◽  
Author(s):  
Witchulada Yungyuen ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Kazuki Yamawaki ◽  
Masaki Yahata ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Citrus Juice ◽  
Different Temperatures ◽  
Juice Sacs

scholarly journals Regulation of ascorbic acid metabolism by blue LED light irradiation in citrus juice sacs

Plant Science ◽  
2015 ◽  
Vol 233 ◽  
pp. 134-142 ◽  
Author(s):  
Lancui Zhang ◽  
Gang Ma ◽  
Kazuki Yamawaki ◽  
Yoshinori Ikoma ◽  
Hikaru Matsumoto ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Light Irradiation ◽  
Blue Led ◽  
Led Light ◽  
Citrus Juice ◽  
Juice Sacs

Regulation of carotenoid metabolism in response to different temperatures in citrus juice sacs in vitro

2018 ◽  
Vol 238 ◽  
pp. 384-390 ◽  
Author(s):  
Witchulada Yungyuen ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Misato Futamura ◽  
Makoto Tabuchi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Metabolism ◽  
Different Temperatures ◽  
Juice Sacs

In vitro sugar uptake by grapefruit (Citrus paradisi) juice-sac cells

2005 ◽  
Vol 32 (4) ◽  
pp. 357 ◽  
Author(s):  
Moshe Huberman ◽  
Uri Zehavi ◽  
Wilfred D. Stein ◽  
Ed Etxeberria ◽  
Raphael Goren
Keyword(s):  
Metabolic Inhibitors ◽  
Sugar Uptake ◽  
Citrus Paradisi ◽  
Citrus Juice ◽  
14Co2 Evolution ◽  
Independent Process ◽  
Initial Uptake ◽  
Juice Sacs ◽  
Slight Inhibition

To further our understanding of the mechanisms of sugar uptake and accumulation into grapefruit (Citrus paradisi Macf. cv. Marsh seedless), the patterns of uptake and utilisation of sucrose, glucose and fructose by Citrus juice cells was investigated. Analyses were conducted on sliced juice sacs that were incubated in radioactive [14C]-sugar solutions with unlabelled sugars, in the presence or absence of metabolic inhibitors. Both hexoses demonstrated an initial uptake peak in December and a second uptake peak in February–March. From March through April the rates of sucrose uptake increased to levels comparable to those of glucose and fructose. Sucrose and its moieties fructose and glucose entered the juice cells of Citrus juice fruit by an insaturable, and mostly by an independent, process. However, NaN3 and carbonylcyanide m-chlorophenylhydrazone (CCCP) produced slight inhibition of these processes. Cells took up hexoses at a greater rate than sucrose, with accumulation reaching a plateau by 4–8 h, and then continuing unabated, in the case of glucose, for 42 h. Uptake of all three sugars increased linearly in the range of sugar concentrations tested, which extended from 0.01 to 320 mm, denoting an insaturable system for sugar uptake. 14CO2 evolution was relatively low in all the experiments, the lowest evolution being recorded when the uptake of [14C]-sucrose was studied, while the highest 14CO2 evolution was recorded when the uptake of [14C]-glucose was studied. The data demonstrate a preferential utilisation of glucose over fructose and sucrose. In all the experiments, the two metabolic inhibitors significantly inhibited the decarboxylation of the three sugars.

scholarly journals The effect of molybdenum on ascorbic acid metabolism in rats

1968 ◽  
Vol 106 (3) ◽  
pp. 633-637 ◽  
Author(s):  
N. Sasmal ◽  
N. C. Kar ◽  
Dipti Mukherjee ◽  
G. C. Chatterjee
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Glucuronic Acid ◽  
Liver Enzymes ◽  
Marked Decrease ◽  
Control Diet ◽  
Ascorbic Acid Content ◽  
Oxidative Breakdown ◽  
Loss Of Weight

1. The effect of dietary molybdenum on the growth rate and also on ascorbic acid metabolism in rats was studied. An excess of dietary molybdenum resulted in growth retardation and loss of weight. Tolerance to molybdenum was affected by the nature of the molybdenum salt administered. 2. Molybdenum ingestion altered certain aspects of ascorbic acid metabolism in rats. The conversion of d-glucuronolactone into l-ascorbic acid in vitro and the oxidative breakdown of l-ascorbic acid by liver enzymes decreased with high molybdenum intakes. The activity of liver uronolactonase was slightly inhibited. The activities of l-gulonate dehydrogenase and l-gulonate decarboxylase were not affected appreciably. 3. Molybdenum supplementation of the control diet resulted in an increase in ascorbic acid content of spleen and adrenal gland, and in a marked decrease in the urinary excretion of ascorbic acid and glucuronic acid. The implications of these findings are discussed.

Fairy Chemicals, 2-Azahypoxanthine and 2-Aza-8-oxohypoxanthine, Regulate Carotenoid Accumulation in Citrus Juice Sacs in Vitro

2015 ◽  
Vol 63 (32) ◽  
pp. 7230-7235 ◽  
Author(s):  
Gang Ma ◽  
Lancui Zhang ◽  
Kazuki Yamawaki ◽  
Masaki Yahata ◽  
Jae-Hoon Choi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Accumulation ◽  
Juice Sacs

scholarly journals Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro

2011 ◽  
Vol 63 (2) ◽  
pp. 871-886 ◽  
Author(s):  
Lancui Zhang ◽  
Gang Ma ◽  
Masaya Kato ◽  
Kazuki Yamawaki ◽  
Toshihiko Takagi ◽  
...  
Keyword(s):  
Citrus Juice ◽  
Carotenoid Accumulation ◽  
Metabolic Genes ◽  
Juice Sacs

In vitro and in vivo reduction of erythrocyte sorbitol by ascorbic acid

Diabetes ◽  
1989 ◽  
Vol 38 (8) ◽  
pp. 1036-1041 ◽  
Author(s):  
J. A. Vinson ◽  
M. E. Staretz ◽  
P. Bose ◽  
H. M. Kassm ◽  
B. S. Basalyga
Keyword(s):  
Ascorbic Acid

IMPACT OF SORBITOL- INDUCED OSMOTIC STRESS ON SOME BIOCHEMICAL TRAITS OF POTATO IN VITRO

2020 ◽  
Vol 51 (4) ◽  
pp. 1038-1047
Author(s):  
Mawia & et al.
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Stress ◽  
Cytoplasmic Membrane ◽  
Genotypic Variation ◽  
Membrane Damage ◽  
Membrane Integrity ◽  
Culture Collection ◽  
Nutritive Medium ◽  
Starting Point

This study had as principal objective identification of osmotic-tolerant potato genotypes by using "in vitro" tissue culture and sorbitol as a stimulating agent, to induce water stress, which was added to the  culture nutritive medium in different concentration (0,50, 110, 220, 330 and 440 mM).  The starting point was represented by plantlets culture collection, belonging to eleven potato genotypes: Barcelona, Nectar, Alison, Jelly, Malice, Nazca, Toronto, Farida, Fabulla, Colomba and Spunta. Plantlets were multiplied between two internodes to obtain microcuttings (in sterile condition), which were inoculated on medium. Sorbitol-induced osmotic stress caused a significant reduction in the ascorbic acid, while the concentration of proline, H2O2 and solutes leakage increased compared with the control. Increased the proline content prevented lipid peroxidation, which played a pivotal role in the maintenance of membrane integrity under osmotic stress conditions. The extent of the cytoplasmic membrane damage depends on osmotic stress severity and the genotypic variation in the maintenance of membranes stability was highly associated with the ability of producing more amounts of osmoprotectants (proline) and the non-enzymic antioxidant ascorbic acid in response to osmotic stress level. The results showed that the genotypes Jelly, Nectar, Allison, Toronto, and Colomba are classified as highly osmotic stress tolerant genotypes, while the genotypes Nazca and Farida are classified as osmotic stress susceptible ones.

Trans-sialidase Associated with Atherosclerosis: Defining the Identity of a Key Enzyme Involved in the Pathology

2019 ◽  
Vol 20 (9) ◽  
pp. 938-941
Author(s):  
Victor Y. Glanz ◽  
Veronika A. Myasoedova ◽  
Andrey V. Grechko ◽  
Alexander N. Orekhov
Keyword(s):  
Acid Metabolism ◽  
Research Subject ◽  
Disease Pathogenesis ◽  
Sialidase Activity ◽  
Ph Values ◽  
Ldl Particles ◽  
Optimum Ph ◽  
Key Enzyme ◽  
St6gal I

Atherosclerosis is associated with the increased trans-sialidase activity, which can be detected in the blood plasma of atherosclerosis patients. The likely involvement in the disease pathogenesis made this activity an interesting research subject and the enzyme that may perform such activity was isolated and characterized in terms of substrate specificity and enzymatic properties. It was found that the enzyme has distinct optimum pH values, and its activity was enhanced by the presence of Ca2+ ions. Most importantly, the enzyme was able to cause atherogenic modification of lowdensity lipoprotein (LDL) particles in vitro. However, the identity of the discovered enzyme remained to be defined. Currently, sialyltransferases, mainly ST6Gal I, are regarded as major contributors to sialic acid metabolism in human blood. In this mini-review, we discuss the possibility that atherosclerosis- associated trans-sialidase does, in fact, belong to the sialyltransferases family.

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