scholarly journals Determination of 70 kDa Proteins for Different Temperatures in Cattle Oocytes Matured in vitro

2019 ◽  
pp. 112-116
Author(s):  
Leyla Bener ◽  
Mehmet Kuran
Keyword(s):  
Different Temperatures

scholarly journals Effects of Sucrose Palmitate on the Physico-Chemical and Mucoadhesive Properties of Buccal Films

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5248
Author(s):  
András Kelemen ◽  
Bálint Katona ◽  
Szilvia Módra ◽  
Zoltán Aigner ◽  
István Sebe ◽  
...  
Keyword(s):  
Hydroxypropyl Methylcellulose ◽  
Permeation Enhancer ◽  
X Ray ◽  
Positron Annihilation Lifetime ◽  
Physico Chemical ◽  
Different Temperatures ◽  
Measured Contact Angle ◽  
Buccal Films

In our current research, sucrose palmitate (SP) was applied as a possible permeation enhancer for buccal use. This route of administration is a novelty as there is no literature on the use of SP in buccal mucoadhesive films. Films containing SP were prepared at different temperatures, with different concentrations of SP and different lengths of hydroxypropyl methylcellulose (HPMC) chains. The mechanical, structural, and in vitro mucoadhesive properties of films containing SP were investigated. Tensile strength and mucoadhesive force were measured with a device and software developed in our Institute. Positron annihilation lifetime spectroscopy (PALS) and X-ray powder diffractometry (XRPD) were applied for the structure analysis of the films. Mucoadhesive work was calculated in two ways: from the measured contact angle and compared with direct mucoadhesive work, which measured mucoadhesive force, which is direct mucoadhesion work. These results correlate linearly with a correlation coefficient of 0.98. It is also novel because it is a new method for the determination of mucoadhesive work.

Sperm production of a pony stallion and the treatment of spermatozoa in vitro with special reference to artificial insemination of mares

1943 ◽  
Vol 33 (2) ◽  
pp. 67-73 ◽  
Author(s):  
Min Chueh Chang
Keyword(s):  
Adverse Effect ◽  
Low Temperature ◽  
Artificial Insemination ◽  
Sperm Production ◽  
New Techniques ◽  
Different Temperatures ◽  
High Level

1. The sperm production of a pony stallion kept a constant high level when the collection of sperms was performed regularly three times a week. There was no adverse effect on the stallion or on the quantity and quality of the sperms. There is a negative correlation between the volume of semen and the concentration. The total number ejaculated remains relatively constant; the volume of accessory fluids is more variable.2. The motility of horse spermatozoa after dilution with eight different kinds of chemical media and stored at different temperatures was studied: Glucose-yolk-phosphate dilutor devised by Lamhert & McKenzie and glucose-yolk-tartrate dilutor devised by the author were found to be the best for the preservation of horse sperms at low temperature. There was not much difference between those diluted samples stored for 24 hr. at 10° C. and those slowly cooled to 1° C. Concentration of the semen by centrifuge is definitely beneficial for the preservation of horse sperms.3. Pregnancies were obtained by the insemination of sperms centrifuged and kept at 1° C. for 24 hr. The sperms of one stallion can be used for a great number of mares if artificial insemination is practised. The adoption of new techniques for the determination of the time of ovulation and for the induction of ovulation is suggested for successful artificial insemination of mares.

scholarly journals DETERMINATION OF CELL TYPE AND HAEMOCYTE MORPHOMETRIC CHARACTERISTICS OF WESTERN AUSTRALIA FRESHWATER CRAYFISH (Cherax cainii) AT DIFFERENT TEMPERATURES IN VITRO

2020 ◽  
Vol 8 (2) ◽  
pp. 1009
Author(s):  
Bambang Widyo Prastowo ◽  
Ricky Lareu ◽  
Rima Caccetta ◽  
Ravi Fotedar
Keyword(s):  
Cell Types ◽  
Freshwater Crayfish ◽  
Forward Scatter ◽  
Cytoplasmic Granules ◽  
Total Hemocyte Count ◽  
Different Temperatures ◽  
Hemocyte Count ◽  
Scatter Parameter

The purpose of this study was to identify and morphologically characterize the hemocyte cell types of freshwater crayfish, Cherax cainii. In addition to morphological observations using a light microscope (LM) and electron transmission microscope (TEM), a flow cytometer (FCM) is also used. Three main types of haemocyte of C. cainii were identified by LM, TEM, and FCM. Determination of haemocyte by LM based on the number, size of cytoplasmic granules and the ratio of N:C. These cells are Hyaline (HC), Small Granule (SGC), and Large Granule (LGC) cells. Three types of haemocyte were also observed by TEM based on cell and nucleus size, granule diameter, number of cytoplasmic granules per cell and N:C. Haemocyte population was successfully detected with FCM based on forward scatter (FSC) signals, versus side scattering signals/side scatter (SSC), with plot data via scatter parameter gating. Three cluster formations were observed, which were temporarily classified as SGC, LGC, and HC regions. Morphometric analysis was performed with TEM on C. cainii haemocyte to measure various cellular features. Some morphological features vary between types of haemocyte and are also affected by temperature. Total hemocyte count (THC) and differential hemocyte count (DHC) are calculated using FCM. THC increases with higher temperatures, from 1,9 x 106 /ml at 20 °C to 4.9 x 106 /ml at 30 °C.  The most abundant hemocyte at all temperatures is HC, followed by SGC and LGC.

Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
W Jülich ◽  
J Pörksen ◽  
H Welzel ◽  
U Lindequist
Keyword(s):  
Aging Effects

In vitro determination of the skin anti-aging potential of eleven South African plants

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
GN Ndlovu ◽  
G Fouche ◽  
W Cordier ◽  
V Steenkamp ◽  
M Tselanyane
Keyword(s):  
South African ◽  
South African Plants ◽  
African Plants

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

Quantitative Assessment of Soluble Fibrin in Plasma by Affinity Chromatography – A Comparative Study with desAA-Fibrin, desAABB-Fibrin and Fibrinogen

1985 ◽  
Vol 54 (02) ◽  
pp. 533-538 ◽  
Author(s):  
Wilfried Thiel ◽  
Ulrich Delvos ◽  
Gert Müller-Berghaus
Keyword(s):  
Affinity Chromatography ◽  
Calcium Ions ◽  
Quantitative Assessment ◽  
Fluid Phase ◽  
Soluble Fibrin ◽  
Binding Characteristics ◽  
Different Temperatures ◽  
Immobilized Proteins ◽  
Sepharose 4B

SummaryA quantitative determination of soluble fibrin in plasma was carried out by affinity chromatography. For this purpose, desAA-fibrin and fibrinogen immobilized on Sepharose 4B were used at the stationary side whereas batroxobin-induced 125I-desAA-fibrin or thrombin-induced 125I-desAABB-fibrin mixed with plasma containing 131I-fibrinogen represented the fluid phase. The binding characteristics of these mixtures to the immobilized proteins were compared at 20° C and 37° C. Complete binding of both types of fibrin to the immobilized desAA-fibrin was always seen at 20° C as well as at 37° C. However, binding of soluble fibrin was accompanied by substantial binding of fibrinogen that was more pronounced at 20° C. Striking differences depending on the temperature at which the affinity chromatography was carried out, were documented for the fibrinogen-fibrin interaction. At 20° C more than 90% of the applied desAA-fibrin was bound to the immobilized fibrinogen whereas at 37° C only a mean of 17% were retained at the fibrinogen-Sepharose column. An opposite finding with regard to the tested temperature was made with the desAABB-fibrin. Nearly complete binding to insolubilized fibrinogen was found at 37° C (95%) but only 58% of the desAABB-fibrin were bound at 20° C. The binding patterns did not change when the experiments were performed in the presence of calcium ions. The opposite behaviour of the two types of soluble fibrin to immobilized fibrinogen at the different temperatures, together with the substantial binding of fibrinogen in the presence of soluble fibrin to insolubilized fibrin in every setting tested, devaluates affinity chromatography as a tool in the quantitative assessment of soluble fibrin in patients’ plasma.

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