scholarly journals The effect of molybdenum on ascorbic acid metabolism in rats

1968 ◽  
Vol 106 (3) ◽  
pp. 633-637 ◽  
Author(s):  
N. Sasmal ◽  
N. C. Kar ◽  
Dipti Mukherjee ◽  
G. C. Chatterjee
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Glucuronic Acid ◽  
Liver Enzymes ◽  
Marked Decrease ◽  
Control Diet ◽  
Ascorbic Acid Content ◽  
Oxidative Breakdown ◽  
Loss Of Weight

1. The effect of dietary molybdenum on the growth rate and also on ascorbic acid metabolism in rats was studied. An excess of dietary molybdenum resulted in growth retardation and loss of weight. Tolerance to molybdenum was affected by the nature of the molybdenum salt administered. 2. Molybdenum ingestion altered certain aspects of ascorbic acid metabolism in rats. The conversion of d-glucuronolactone into l-ascorbic acid in vitro and the oxidative breakdown of l-ascorbic acid by liver enzymes decreased with high molybdenum intakes. The activity of liver uronolactonase was slightly inhibited. The activities of l-gulonate dehydrogenase and l-gulonate decarboxylase were not affected appreciably. 3. Molybdenum supplementation of the control diet resulted in an increase in ascorbic acid content of spleen and adrenal gland, and in a marked decrease in the urinary excretion of ascorbic acid and glucuronic acid. The implications of these findings are discussed.

scholarly journals Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice

2020 ◽  
Vol 11 ◽  
Author(s):  
Antonella Smeriglio ◽  
Marcella Denaro ◽  
Valeria D’Angelo ◽  
Maria Paola Germanò ◽  
Domenico Trombetta
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Ascorbic Acid Content ◽  
Zebrafish Embryos ◽  
Anti Inflammatory ◽  
Dose Dependent

Citrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancient Mediterranean species, Citrus lumia Risso (CLJ). The antioxidant and anti-inflammatory activities were evaluated by several in vitro cell-free and cell-based assays, whereas two different in vivo models, the chick chorioallantoic membrane (CAM) and the zebrafish embryos, were used to characterize the anti-angiogenic properties. Twenty-eight polyphenols were identified by RP-LC-DAD-ESI-MS analysis (flavonoids 68.82% and phenolic acids 31.18%) with 1-caffeoyl-5-feruloylquinic acid and kaempferol 3′-rhamnoside, which represent the most abundant compounds (25.70 and 23.12%, respectively). HPLC-DAD analysis showed a high ascorbic acid content (352 mg/kg of CLJ), which contributes with polyphenols to the marked and dose-dependent antioxidant and anti-inflammatory properties observed. CLJ showed strong and dose-dependent anti-angiogenic activity as highlighted by the inhibition of blood vessel formation on CAMs and the decrease of endogenous alkaline phosphatase on zebrafish embryos. Moreover, within the concentration range tested, no dead or malformed embryos were recorded. Certainly, further studies are needed to investigate the molecular mechanisms underlying these promising biological effects, but considering the evidence of the present study, the use of CLJ as a ready-to drink safe prevention strategy for inflammatory-based diseases correlated to angiogenesis could be justified.

scholarly journals Obtaining Salt Stress-Tolerant Eggplant Somaclonal Variants from In Vitro Selection

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2539
Author(s):  
Sami Hannachi ◽  
Stefaan Werbrouck ◽  
Insaf Bahrini ◽  
Abdelmuhsin Abdelgadir ◽  
Hira Affan Siddiqui ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Water Content ◽  
Acid Content ◽  
In Vitro Selection ◽  
Callus Tissue ◽  
Salt Content ◽  
Ascorbic Acid Content ◽  
Control Line

An efficient regeneration protocol was applied to regenerate shoots on salt stress-tolerant calli lines of aubergine (Solanum melongena). These NaCl-tolerant cell lines were obtained by two different methods. On the one hand, the developed callus tissue was transferred to a medium with a continuous salt content of 40, 80, 120, or 160 mM NaCl. On the other hand, the callus tissue was subjected to a stepwise increasing salinity to 160 mM NaCl every 30 days. With the second method, calli which could be selected were characterized by compact growth, a greenish color, and absence of necrotic zones. When grown on salt-free medium again, NaCl-tolerant calli showed a decline in relative growth rate and water content in comparison to the control line. This was more obvious in the 120 mM NaCl-tolerant callus. Lipid peroxidase activity increased in 40 and 80 mM NaCl-tolerant calli; yet did not increase further in 120 mM-tolerant callus. An increase in ascorbic acid content was observed in 80 and 120 mM NaCl-tolerant calli compared to the 40 mM NaCl-tolerant lines, in which ascorbic acid content was twice that of the control. All NaCl-tolerant lines showed significantly higher superoxide dismutase (SOD) (208–305–370 µmol min−1 mg−1 FW) and catalase (CAT) (136–211–238 µmol min−1 mg−1 FW) activities compared to control plants (231 and 126 µmol min−1 mg−1 FW). Plants were regenerated on the calli lines that could tolerate up to 120 mM NaCl. From the 32 plants tested in vitro, ten plants with a higher number of leaves and root length could be selected for further evaluation in the field. Their high salt tolerance was evident by their more elevated fresh and dry weight, their more increased relative water content, and a higher number and weight of fruits compared to the wild-type parental control. The presented work shows that somaclonal variation can be efficiently used to develop salt-tolerant mutants.

Anoxia-resistant turtle brain maintains ascorbic acid content in vitro

1991 ◽  
Vol 132 (2) ◽  
pp. 141-145 ◽  
Author(s):  
Margaret E. Rice ◽  
Jon Cammack
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Ascorbic Acid Content

THE ADRENAL REPONSE OF HAMSTERS, GERBILS AND RATS TO EXOGENOUS CORTICOTROPHIN OR UNILATERAL ADRENALECTOMY

1967 ◽  
Vol 54 (2) ◽  
pp. 257-262 ◽  
Author(s):  
Paul F. Robinson ◽  
Russel J. Reiter
Keyword(s):  
Ascorbic Acid ◽  
Adrenal Gland ◽  
Mongolian Gerbil ◽  
Acid Content ◽  
Marked Decrease ◽  
Ascorbic Acid Content ◽  
Unilateral Adrenalectomy

ABSTRACT Either unilateral or sham adrenalectomy caused a marked decrease in the ascorbic acid content of the remaining adrenal gland of the rat. An even greater drop of this constituent was obtained one hour after the administration of 10 IU of corticotrophin. Rat adrenal cholesterol was somewhat less responsive to the above treatment. Unilateral or sham adrenalectomy had no significant effect on adrenal ascorbic acid or cholesterol in the hamster. Twenty IU of corticotrophin caused a 20% drop in hamster adrenal cholesterol whereas 10 IU did not. The response of the Mongolian gerbil to corticotrophin or adrenalectomy, either unilateral or sham, was approximately the same as that of the rat.

scholarly journals IN VITRO ANTIOXIDANT CAPACITY, PHENOLIC, ASCORBIC ACID AND LYCOPENE CONTENT OF GUAVA (Psidium guajava L.) JUICES AND NECTARS

2009 ◽  
Vol 27 (2) ◽  
Author(s):  
CARLOS ALEXANDRE KOGUISHI DE BRITO ◽  
PRISCILA BECKER SIQUEIRA ◽  
JANE CRISTINA DE SOUZA ◽  
HELENA MARIA ANDRÉ BOLINI
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Ascorbic Acid Content ◽  
Psidium Guajava ◽  
Polyphenolic Compounds ◽  
Polyphenolic Content ◽  
Total Polyphenolic Content ◽  
Lycopene Content ◽  
Free Radicals Scavenging

capacity in vitro of three different brands of guava nectars and juices, through free radicals scavenging methods, 1,1-diphenyl-2-picrylhydrazine (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS.+), and its correlation with the total polyphenolic content, total lycopene and ascorbic acid. Brands revealed statistical differences (p£ 0.05) in antioxidant capacity, ranging from 1.9 to 7.7 molTE/mL. Antioxidant capacity presented positive correlation for ascorbic acid content and polyphenolic compounds, being relatively low for the lycopene. Different process can influence the content of these compounds as well as interfere in their antioxidant capacity. The control of the production process is important to add value to guava products and fulfill the new tendency of the market.

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