Genetic transformation of Prunus domestica L. using the hpt gene coding for hygromycin resistance as the selectable marker

2009 ◽  
Vol 119 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Lining Tian ◽  
Fatih Ali Canli ◽  
Xinhua Wang ◽  
Susan Sibbald
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Hygromycin Resistance ◽  
Prunus Domestica ◽  
Gene Coding

scholarly journals The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae

Metabolites ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 49 ◽  
Author(s):  
Ana Molina-Márquez ◽  
Marta Vila ◽  
Javier Vigara ◽  
Ana Borrero ◽  
Rosa León
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Genetic Manipulation ◽  
Plant Cells ◽  
Phytoene Desaturase ◽  
Carotenoid Content ◽  
Great Promise ◽  
Encoding Gene ◽  
Average Transformation ◽  
Selection Of

Genetic manipulation shows great promise to further boost the productivity of microalgae-based compounds. However, selection of microalgal transformants depends mainly on the use of antibiotics, which have raised concerns about their potential impacts on human health and the environment. We propose the use of a synthetic phytoene desaturase-encoding gene (CRTIop) as a selectable marker and the bleaching herbicide norflurazon as a selective agent for the genetic transformation of microalgae. Bacterial phytoene desaturase (CRTI), which, unlike plant and algae phytoene desaturase (PDS), is not sensitive to norflurazon, catalyzes the conversion of the colorless carotenoid phytoene into lycopene. Although the expression of CRTI has been described to increase the carotenoid content in plant cells, its use as a selectable marker has never been testedin algae or in plants. In this study, a version of the CRTI gene adapted to the codon usage of Chlamydomonas has been synthesized, and its suitability to be used as selectable marker has been shown. The microalgae were transformed by the glass bead agitation method and selected in the presence of norflurazon. Average transformation efficiencies of 550 colonies µg−1 DNA were obtained. All the transformants tested had incorporated the CRTIop gene in their genomes and were able to synthesize colored carotenoids.

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

1985 ◽  
Vol 5 (5) ◽  
pp. 299-302 ◽  
Author(s):  
Peter J. M. van den Elzen ◽  
Jeffrey Townsend ◽  
Kathleen Y. Lee ◽  
John R. Bedbrook
Keyword(s):  
Resistance Gene ◽  
Selectable Marker ◽  
Plant Cells ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Development of a Simvastatin Selection Marker for a Hyperthermophilic Acidophile, Sulfolobus islandicus

2011 ◽  
Vol 78 (2) ◽  
pp. 568-574 ◽  
Author(s):  
Tao Zheng ◽  
Qihong Huang ◽  
Changyi Zhang ◽  
Jinfeng Ni ◽  
Qunxin She ◽  
...  
Keyword(s):  
Selectable Marker ◽  
Gene Knockout ◽  
Plasmid Vector ◽  
Selection Marker ◽  
Content Type ◽  
Sulfolobus Islandicus ◽  
Gene Coding ◽  
High Level ◽  
Coa Reductase

ABSTRACTWe report here a novel selectable marker for the hyperthermophilic crenarchaeonSulfolobus islandicus. The marker cassette is composed of thesac7dpromoter and thehmggene coding for the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (Psac7d-hmg), which confers simvastatin resistance to this crenarchaeon. The basic plasmid vector pSSR was constructed by substituting thepyrEFgene of the expression vector pSeSD for Psac7d-hmgwith which theSulfolobusexpression plasmids pSSRlacS, pSSRAherA, and pSSRNherA were constructed. Characterization ofSulfolobustransformants carrying pSSRlacS indicated that the plasmid was properly maintained under selection. High-level expression of the His6-tagged HerA helicase was obtained with the cells harboring pSSRAherA. The establishment of two efficient selectable markers (pyrEFandhmg) was subsequently exploited for genetic analysis. AherAmerodiploid strain ofS. islandicuswas constructed usingpyrEFmarker and used as the host to obtain pSSRNherA transformant with simvastatin selection. While the gene knockout (ΔherA) cells generated from theherAmerodiploid cells failed to form colonies in the presence of 5-fluoroorotic acid (5-FOA), the mutant cells could be rescued by expression of the gene from a plasmid (pSSRNherA), because their transformants formed colonies on a solid medium containing 5-FOA and simvastatin. This demonstrates that HerA is essential for cell viability ofS. islandicus. To our knowledge, this is the first application of an antibiotic selectable marker in genetic study for a hyperthermophilic acidophile and in the crenarchaeal lineage.

scholarly journals Agrobacterium-mediated Genetic Transformation of Potato (Solanum tuberosum L.) var. Cardinal and Heera

2012 ◽  
Vol 10 (1) ◽  
pp. 81-86 ◽  
Author(s):  
A. Khatun ◽  
M. M. Hasan ◽  
M. A. A. Bachchu ◽  
M. Moniruzzaman ◽  
K. M. Nasiruddin
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Binary Vector ◽  
Marker Gene ◽  
Kanamycin Resistance ◽  
Histochemical Analysis ◽  
Gene Encoding ◽  
Antisense Gene ◽  
Npt Ii ◽  
Transformation Experiment

Two potato varieties namely Cardinal and Heera were used in the Agrobacterium-mediated genetic transformation experiment to investigate the genetic transformation ability in the Biotechnology laboratory of the Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh during 2006 to 2007. Agrobacterium tumefaciens strain LBA 4404 having a binary vector pB1121 of 14 KDa containing selectable marker gene npt II (neomycine phosphotransferase II) conferring kanamycin resistance, and the CIPK antisense gene encoding calcineurin B-like protein were used. Leaf and internodes were used as explants. Expression of the transgene (GUS) was confirmed by histochemical analysis. The variety Cardinal was found more suitable for expressing best GUS response (80% GUS positive) over Heera.DOI: http://dx.doi.org/10.3329/agric.v10i1.11068The Agriculturists 2012; 10(1): 81-86

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja ◽  
Hassan Riaz ◽  
Muhsin Jamal ◽  
Muhammad Imran
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.   Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran

scholarly journals Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation

10.5772/47071 ◽  
2011 ◽  
Author(s):  
Yuan Song ◽  
Fatih Ali ◽  
Farida Meerja ◽  
Xinhua Wang ◽  
Hugh A. L. Henry ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Prunus Domestica ◽  
Factors Affecting
Sign in / Sign up

Export Citation Format

Share Document