scholarly journals Genetic Transformation of Transcription Factor (35S-oshox4) Gene into Rice Genome and Transformant Analysis of hpt Gene by PCR and Hygromycin Resistance Test

2009 ◽  
Vol 10 (2) ◽  
pp. 63-69
Author(s):  
ENUNG SRI MULYANINGSIH
Keyword(s):  
Transcription Factor ◽  
Genetic Transformation ◽  
Rice Genome ◽  
Resistance Test ◽  
Hygromycin Resistance

scholarly journals Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK

2002 ◽  
Vol 184 (9) ◽  
pp. 2344-2351 ◽  
Author(s):  
Mitsuo Ogura ◽  
Hirotake Yamaguchi ◽  
Kazuo Kobayashi ◽  
Naotake Ogasawara ◽  
Yasutaro Fujita ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Genetic Transformation ◽  
Dna Microarray ◽  
Genome Analysis ◽  
Transformation Efficiency ◽  
The Other ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Microarray Technique

ABSTRACT The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known previously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.

scholarly journals Agrobacterium-mediated Genetic Transformation of Rice var. BRRI Dhan 58

2021 ◽  
Vol 31 (1) ◽  
pp. 71-80
Author(s):  
Mst Sufara Akhter Banu ◽  
Bulbul Ahmed ◽  
Shahanaz Parveen ◽  
Md Harun Ur Rashid ◽  
Kazi Md Kamrul Huda
Keyword(s):  
Genetic Transformation ◽  
Rice Plant ◽  
Crop Improvement ◽  
Marker Genes ◽  
Hygromycin Resistance ◽  
Ms Medium ◽  
Gus Gene ◽  
Functional Studies ◽  
Regeneration Response ◽  
Modified Ms Medium

Agrobacterium mediated genetic transformation of BRRI Dhan 58 was conducted by using immature embryos following indirect regeneration. High percentage of callus induction at 96.5% was obtained when seeds of BRRI dhan 58 were cultured on modified MS medium supplemented with 2.5 mg/l 2, 4-D under dark condition. The maximum regeneration response was rerecorded when MS was supplemented with 3 mg/l BAP + 0.5 mg/l NAA and 1.0 mg/l Kn. Genetic transformation was performed using A. tumefaciens strain LBA4404 harboring pCAMBIA1301 plasmid carrying the marker genes for β-glucuronidase (GUS) and hygromycin resistance (hptII). Integration of the GUS gene into the genome of the rice plants was confirmed by PCR. The leaf segments of the PCR positive transformed plants showed the expression of GUS. The results of this study would be an effective tool for crop improvement and functional studies of gene on rice plant. Plant Tissue Cult. & Biotech. 31(1): 71-80, 2021 (June)

scholarly journals AP2/ERF Transcription Factor in Rice: Genome-Wide Canvas and Syntenic Relationships between Monocots and Eudicots

2012 ◽  
Vol 8 ◽  
pp. EBO.S9369 ◽  
Author(s):  
Muhammad Rashid ◽  
He Guangyuan ◽  
Yang Guangxiao ◽  
Javeed Hussain ◽  
Yan Xu
Keyword(s):  
Transcription Factor ◽  
Rice Genome ◽  
Genome Wide ◽  
Erf Transcription Factor

scholarly journals Genome-Wide Analysis of the RAV Transcription Factor Genes in Rice Reveals Their Response Patterns to Hormones and Virus Infection

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 752
Author(s):  
Changhai Chen ◽  
Yanjun Li ◽  
Hehong Zhang ◽  
Qiang Ma ◽  
Zhongyan Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Virus Infection ◽  
Rice Genome ◽  
Rice Stripe Virus ◽  
Response Patterns ◽  
Chromosomal Distribution ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Genome Wide ◽  
Transcription Factor Genes

The RAV family is part of the B3 superfamily and is one of the most abundant transcription factor families in plants. Members have highly conserved B3 or AP2 DNA binding domains. Although the RAV family genes of several species have been systematically identified from genome-wide studies, there has been no comprehensive study to identify rice RAV family genes. Here, we identified 15 genes of the RAV family in the rice genome and analyzed their phylogenetic relationships, gene structure, conserved domains, and chromosomal distribution. Based on domain similarity and phylogenetic topology, rice RAV transcription factors were phylogenetically clustered into four groups. qRT-PCR analyses showed that expression of these RAV genes was significantly up-regulated or down-regulated by plant hormone treatments, including BL, NAA, IAA, MeJA, and SA. Most of the rice RAV genes were dramatically down-regulated in response to rice stripe virus (RSV) and mostly up-regulated in response to Southern rice black-streaked dwarf virus (SRBSDV). These results suggest that the rice RAV genes are involved in diverse signaling pathways and in varied responses to virus infection.

scholarly journals Atypical Splicing Accompanied by Skipping Conserved Micro-exons Produces Unique WRINKLED1, An AP2 Domain Transcription Factor in Rice Plants

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 207 ◽  
Author(s):  
Mano ◽  
Aoyanagi ◽  
Kozaki
Keyword(s):  
Transcription Factor ◽  
Amino Acid ◽  
Rice Genome ◽  
Amino Acid Replacement ◽  
Rice Plants ◽  
Plant Seeds ◽  
Oil Synthesis ◽  
Related Proteins ◽  
Ap2 Domain ◽  
Arabidopsis Cells

WRINKLED1 (WRI1), an AP2 domain transcription factor, is a master regulator of oil synthesis in plant seeds. Its closely related proteins (WRIs) are also involved in regulating the synthesis of fatty acids, which play a role in producing oils, membranes, and other important components in plants. We found two WRI1 genes, OsWRI1-1 and OsWRI1-2, and two additional WRI1 homologs, OsWRI3 and OsWRI4, in the rice genome. OsWRI1 was ubiquitously expressed in rice plants, including developing seeds. However, OsWRI3 was only significantly expressed in the leaf blade and OsWRI4 was not expressed at all. OsWRI1-1 contains amino acid sequence GCL instead of VYL, which is encoded by an independent 9-bp micro-exon that is conserved in many plant species. We found that the GCL sequence was produced by an atypical splicing accompanied by skipping of the micro-exon. Furthermore, OsWRI1-1 highly activates the transcription of the promoter for the biotin carboxyl transferase 2 gene in Arabidopsis, but its activity was reduced by amino acid replacement or deletion of the GCL sequence in a transient assay using Arabidopsis cells. Our results indicated that atypical splicing produced unique WRI1 in rice plants.

scholarly journals Genetic transformation and growth index determination of the Larix olgensis LoHDZ2 transcription factor gene in tobacco

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Peiqi An ◽  
Chen Wang ◽  
Qing Cao ◽  
Qingrong Zhao ◽  
Ruofan Qin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Genetic Transformation ◽  
Growth Index ◽  
Hormone Treatment ◽  
Larix Olgensis ◽  
Transcription Factor Gene ◽  
Leucine Zippers ◽  
Factor Gene ◽  
Dna And Rna ◽  
Development Processes

AbstractHomeodomain-leucine zippers (HD-Zip) are plant-specific transcription factors that participate in different plant development processes and differentially regulate metabolic processes. LoHDZ2 is an HD-ZipII subfamily transcription factor gene that we identified from a transcriptomic analysis of Larix olgensis. To understand its function, we built a LoHDZ2 expression vector and then inserted it into tobacco by genetic transformation. Transgenic plants were identified at the DNA and RNA levels. Phenotypic index analysis of transgenic tobacco showed dwarfed growth with larger leaves and earlier flowering than the wild type. LoHDZ2 was expressed differently after hormone treatment with IAA, MeJA and 2,4-D. The results suggested that LoHDZ2 may respond to hormones and be involved in regulating growth and metabolism. These results helped us better understand the function of LoHDZ2 and provided a candidate gene for Larix olgensis molecular breeding.

scholarly journals Comparative genome-wide analysis of WRKY, MADS-box and MYB transcription factor families in Arabidopsis and rice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad-Redha Abdullah-Zawawi ◽  
Nur-Farhana Ahmad-Nizammuddin ◽  
Nisha Govender ◽  
Sarahani Harun ◽  
Norfarhan Mohd-Assaad ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Structure ◽  
Stress Responses ◽  
Biotic Stress ◽  
Rice Genome ◽  
Model Systems ◽  
Mads Box ◽  
Abiotic And Biotic Stress ◽  
Developmental Processes ◽  
Gene Pairs

AbstractTranscription factors (TFs) form the major class of regulatory genes and play key roles in multiple plant stress responses. In most eukaryotic plants, transcription factor (TF) families (WRKY, MADS-box and MYB) activate unique cellular-level abiotic and biotic stress-responsive strategies, which are considered as key determinants for defense and developmental processes. Arabidopsis and rice are two important representative model systems for dicot and monocot plants, respectively. A comprehensive comparative study on 101 OsWRKY, 34 OsMADS box and 122 OsMYB genes (rice genome) and, 71 AtWRKY, 66 AtMADS box and 144 AtMYB genes (Arabidopsis genome) showed various relationships among TFs across species. The phylogenetic analysis clustered WRKY, MADS-box and MYB TF family members into 10, 7 and 14 clades, respectively. All clades in WRKY and MYB TF families and almost half of the total number of clades in the MADS-box TF family are shared between both species. Chromosomal and gene structure analysis showed that the Arabidopsis-rice orthologous TF gene pairs were unevenly localized within their chromosomes whilst the distribution of exon–intron gene structure and motif conservation indicated plausible functional similarity in both species. The abiotic and biotic stress-responsive cis-regulatory element type and distribution patterns in the promoter regions of Arabidopsis and rice WRKY, MADS-box and MYB orthologous gene pairs provide better knowledge on their role as conserved regulators in both species. Co-expression network analysis showed the correlation between WRKY, MADs-box and MYB genes in each independent rice and Arabidopsis network indicating their role in stress responsiveness and developmental processes.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

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