A chimaeric hygromycin resistance gene as a selectable marker in plant cells

1985 ◽  
Vol 5 (5) ◽  
pp. 299-302 ◽  
Author(s):  
Peter J. M. van den Elzen ◽  
Jeffrey Townsend ◽  
Kathleen Y. Lee ◽  
John R. Bedbrook
Keyword(s):  
Resistance Gene ◽  
Selectable Marker ◽  
Plant Cells ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

A mutated hygromycin resistance gene is functional in the n -alkane-assimilating yeast Candida tropicalis

2000 ◽  
Vol 173 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Akihiro Hara ◽  
Mitsuyoshi Ueda ◽  
Satoru Misawa ◽  
Toru Matsui ◽  
Keizo Furuhashi ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Candida Tropicalis ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Hygromycin Resistance Gene Cassettes for Vector Construction and Selection of Transformed Rice Protoplasts

1991 ◽  
Vol 97 (2) ◽  
pp. 832-835 ◽  
Author(s):  
Zhenwei Zheng ◽  
Akio Hayashimoto ◽  
Zhijian Li ◽  
Norimoto Murai
Keyword(s):  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Gene Cassettes ◽  
Vector Construction ◽  
Hygromycin Resistance Gene ◽  
Selection Of

scholarly journals Hygromycin resistance as a selectable marker in Dictyostelium discoideum.

1989 ◽  
Vol 9 (5) ◽  
pp. 1965-1968 ◽  
Author(s):  
T T Egelhoff ◽  
S S Brown ◽  
D J Manstein ◽  
J A Spudich
Keyword(s):  
Dictyostelium Discoideum ◽  
High Efficiency ◽  
Selectable Marker ◽  
Hygromycin Resistance ◽  
Origin Of Replication ◽  
Transcription Terminator ◽  
G418 Resistance ◽  
Hygromycin Selection ◽  
Hygromycin Resistance Gene ◽  
Transformed Cell Lines

We have constructed an expression cartridge which has the bacterial hygromycin resistance gene (hph) fused to the Dictyostelium discoideum actin 15 promoter, with a segment of 3'-flanking DNA from the actin 15 locus placed downstream of the hph gene to serve as a transcription terminator. The plasmid pDE109, which contained this cartridge and a Dictyostelium origin of replication, transformed D. discoideum with high efficiency under hygromycin selection. The availability of this selectable marker circumvents the previous limitation of having G418 resistance as the only selectable marker for this organism; secondary transformation can now be used to introduce DNA into previously transformed cell lines.

Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph

Gene ◽  
1987 ◽  
Vol 57 (1) ◽  
pp. 21-26 ◽  
Author(s):  
D. Cullen ◽  
S.A. Leong ◽  
L.J. Wilson ◽  
D.J. Henner
Keyword(s):  
Aspergillus Nidulans ◽  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Stability and modulated expression of a hygromycin resistance gene integrated in Botrytis cinerea transformants

2006 ◽  
Vol 154 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Walid Hamada ◽  
Marie-Christine Soulié ◽  
Pierrette Malfatti ◽  
Gilbert Bompeix ◽  
Martine Boccara
Keyword(s):  
Botrytis Cinerea ◽  
Resistance Gene ◽  
Hygromycin Resistance ◽  
Hygromycin Resistance Gene

scholarly journals Transfer of a Supernumerary Chromosome Between Vegetatively Incompatible Biotypes of the Fungus Colletotrichum gloeosporioides

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1459-1466 ◽  
Author(s):  
Chaozu He ◽  
Anca G Rusu ◽  
Agnieszka M Poplawski ◽  
John A G Irwin ◽  
John M Manners
Keyword(s):  
Resistance Gene ◽  
Colletotrichum Gloeosporioides ◽  
Mixed Cultures ◽  
Marker Genes ◽  
Hygromycin Resistance ◽  
Antibiotic Resistant ◽  
Selectable Marker Genes ◽  
Tropical Legumes ◽  
Hygromycin Resistance Gene ◽  
Biotype B

Abstract Two biotypes (A and B) of Colletotrichum gloeosporioides infect the tropical legumes Stylosanthes spp. in Australia. These biotypes are asexual and vegetatively incompatible. However, field isolates of biotype B carrying a supernumerary 2-Mb chromosome, thought to originate from biotype A, have been reported previously. We tested the hypothesis that the 2-Mb chromosome could be transferred from biotype A to biotype B under laboratory conditions. Selectable marker genes conferring resistance to hygromycin and phleomycin were introduced into isolates of biotypes A and B, respectively. A transformant of biotype A, with the hygromycin resistance gene integrated on the 2-Mb chromosome, was cocultivated with phleomycin-resistant transformants of biotype B. Double antibiotic-resistant colonies were obtained from conidia of these mixed cultures at a frequency of approximately 10-7. Molecular analysis using RFLPs, RAPDs, and electrophoretic karyotypes showed that these colonies contained the 2-Mb chromosome in a biotype B genetic background. In contrast, no double antibiotic colonies developed from conidia obtained from mixed cultures of phleomycin-resistant transformants of biotype B with biotype A transformants carrying the hygromycin resistance gene integrated in chromosomes >2 Mb in size. The results demonstrated that the 2-Mb chromosome was selectively transferred from biotype A to biotype B. The horizontal transfer of specific chromosomes across vegetative incompatibility barriers may explain the origin of supernumerary chromosomes in fungi.

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