A counter-selectable marker for genetic transformation of the yeast Schwanniomyces alluvius

1997 ◽  
Vol 48 (2) ◽  
pp. 204-207 ◽  
Author(s):  
M. N. Dave ◽  
B. B. Chattoo
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker

scholarly journals The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae

Metabolites ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 49 ◽  
Author(s):  
Ana Molina-Márquez ◽  
Marta Vila ◽  
Javier Vigara ◽  
Ana Borrero ◽  
Rosa León
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Genetic Manipulation ◽  
Plant Cells ◽  
Phytoene Desaturase ◽  
Carotenoid Content ◽  
Great Promise ◽  
Encoding Gene ◽  
Average Transformation ◽  
Selection Of

Genetic manipulation shows great promise to further boost the productivity of microalgae-based compounds. However, selection of microalgal transformants depends mainly on the use of antibiotics, which have raised concerns about their potential impacts on human health and the environment. We propose the use of a synthetic phytoene desaturase-encoding gene (CRTIop) as a selectable marker and the bleaching herbicide norflurazon as a selective agent for the genetic transformation of microalgae. Bacterial phytoene desaturase (CRTI), which, unlike plant and algae phytoene desaturase (PDS), is not sensitive to norflurazon, catalyzes the conversion of the colorless carotenoid phytoene into lycopene. Although the expression of CRTI has been described to increase the carotenoid content in plant cells, its use as a selectable marker has never been testedin algae or in plants. In this study, a version of the CRTI gene adapted to the codon usage of Chlamydomonas has been synthesized, and its suitability to be used as selectable marker has been shown. The microalgae were transformed by the glass bead agitation method and selected in the presence of norflurazon. Average transformation efficiencies of 550 colonies µg−1 DNA were obtained. All the transformants tested had incorporated the CRTIop gene in their genomes and were able to synthesize colored carotenoids.

scholarly journals Agrobacterium-mediated Genetic Transformation of Potato (Solanum tuberosum L.) var. Cardinal and Heera

2012 ◽  
Vol 10 (1) ◽  
pp. 81-86 ◽  
Author(s):  
A. Khatun ◽  
M. M. Hasan ◽  
M. A. A. Bachchu ◽  
M. Moniruzzaman ◽  
K. M. Nasiruddin
Keyword(s):  
Genetic Transformation ◽  
Selectable Marker ◽  
Binary Vector ◽  
Marker Gene ◽  
Kanamycin Resistance ◽  
Histochemical Analysis ◽  
Gene Encoding ◽  
Antisense Gene ◽  
Npt Ii ◽  
Transformation Experiment

Two potato varieties namely Cardinal and Heera were used in the Agrobacterium-mediated genetic transformation experiment to investigate the genetic transformation ability in the Biotechnology laboratory of the Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh during 2006 to 2007. Agrobacterium tumefaciens strain LBA 4404 having a binary vector pB1121 of 14 KDa containing selectable marker gene npt II (neomycine phosphotransferase II) conferring kanamycin resistance, and the CIPK antisense gene encoding calcineurin B-like protein were used. Leaf and internodes were used as explants. Expression of the transgene (GUS) was confirmed by histochemical analysis. The variety Cardinal was found more suitable for expressing best GUS response (80% GUS positive) over Heera.DOI: http://dx.doi.org/10.3329/agric.v10i1.11068The Agriculturists 2012; 10(1): 81-86

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

scholarly journals Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja ◽  
Hassan Riaz ◽  
Muhsin Jamal ◽  
Muhammad Imran
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.   Copyright (c) 2021 Malik Nawaz Shuja, Hasan Riaz, Muhsin Jamal, Muhammad Imran

Mutant acetolactate synthase gene is an efficient in vitro selectable marker for the genetic transformation of Brassica juncea (oilseed mustard)

2004 ◽  
Vol 161 (9) ◽  
pp. 1079-1083 ◽  
Author(s):  
Krishna Ray ◽  
Arun Jagannath ◽  
Suveena Arora Gangwani ◽  
Pradeep Kumar Burma ◽  
Deepak Pental
Keyword(s):  
Genetic Transformation ◽  
Brassica Juncea ◽  
Selectable Marker ◽  
Acetolactate Synthase

scholarly journals ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11809
Author(s):  
Richard Dormatey ◽  
Chao Sun ◽  
Kazim Ali ◽  
Sajid Fiaz ◽  
Derong Xu ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Herbicide Resistance ◽  
Selectable Marker ◽  
Selection Marker ◽  
Marker Genes ◽  
Selection System ◽  
Efficient System ◽  
Marker System ◽  
Selectable Marker Genes

Antibiotic and herbicide resistance genes are the most common marker genes for plant transformation to improve crop yield and food quality. However, there is public concern about the use of resistance marker genes in food crops due to the risk of potential gene flow from transgenic plants to compatible weedy relatives, leading to the possible development of “superweeds” and antibiotic resistance. Several selectable marker genes such as aph, nptII, aaC3, aadA, pat, bar, epsp and gat, which have been synthesized to generate transgenic plants by genetic transformation, have shown some limitations. These marker genes, which confer antibiotic or herbicide resistance and are introduced into crops along with economically valuable genes, have three main problems: selective agents have negative effects on plant cell proliferation and differentiation, uncertainty about the environmental effects of many selectable marker genes, and difficulty in performing recurrent transformations with the same selectable marker to pyramid desired genes. Recently, a simple, novel, and affordable method was presented for plant cells to convert non-metabolizable phosphite (Phi) to an important phosphate (Pi) for developing cells by gene expression encoding a phosphite oxidoreductase (PTXD) enzyme. The ptxD gene, in combination with a selection medium containing Phi as the sole phosphorus (P) source, can serve as an effective and efficient system for selecting transformed cells. The selection system adds nutrients to transgenic plants without potential risks to the environment. The ptxD/Phi system has been shown to be a promising transgenic selection system with several advantages in cost and safety compared to other antibiotic-based selection systems. In this review, we have summarized the development of selection markers for genetic transformation and the potential use of the ptxD/Phi scheme as an alternative selection marker system to minimize the future use of antibiotic and herbicide marker genes.

Kanamycin in Cereal Biotechnology: A Screening or a Selectable Marker?

2021 ◽  
Vol 3 (1) ◽  
pp. 01-05
Author(s):  
Malik Shuja
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Selectable Marker ◽  
Normal Growth ◽  
Minimal Effect ◽  
Effective Screening ◽  
Transformed Plants ◽  
Transformation Systems ◽  
Selection Agent ◽  
Screening Marker

Kanamycin is a widely used selection agent in dicot-plant genetic transformation systems. In monocots, however, it does not seem to be effective as it has no or minimal effect on the normal growth of non-transformed plants. Kanamycin was previously demonstrated to bleach the pigments of the non-transgenic plants. This may yield the idea that kanamycin can be used as an effective screening marker rather than a selectable marker in monocots.

Genetic transformation of Trametes versicolor to phleomycin resistance with the dominant selectable marker shble

2001 ◽  
Vol 56 (1-2) ◽  
pp. 201-204 ◽  
Author(s):  
K. Bartholomew ◽  
G. Dos Santos ◽  
T. Dumonceaux ◽  
T. Charles ◽  
F. Archibald
Keyword(s):  
Genetic Transformation ◽  
Trametes Versicolor ◽  
Selectable Marker ◽  
Dominant Selectable Marker
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