CYTOPLASMIC LIPID DROPLET ACCUMULATION IS HIGHER IN SINONASAL MELANOMAS THAN IN ORAL AND CUTANEOUS MELANOMAS

2017 ◽  
Vol 124 (2) ◽  
pp. e130
Author(s):  
JULIANA DE SOUZA DO NASCIMENTO ◽  
HARIM TAVARES DOS SANTOS ◽  
FERNANDA VIVIANE MARIANO ◽  
ERIKA SAID ABU EGAL ◽  
FELIPE PAIVA FONSECA ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Cytoplasmic Lipid Droplet

scholarly journals Human Subtilisin Kexin Isozyme-1 (SKI-1)/Site-1 Protease (S1P) regulates cytoplasmic lipid droplet abundance: A potential target for indirect-acting anti-dengue virus agents

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0174483 ◽  
Author(s):  
Anastasia Hyrina ◽  
Fanrui Meng ◽  
Steven J. McArthur ◽  
Sharlene Eivemark ◽  
Ivan R. Nabi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Lipid Droplet ◽  
Potential Target ◽  
Cytoplasmic Lipid Droplet

Lipogenic Enzymes Complexes and Cytoplasmic Lipid Droplet Formation During Adipogenesis

2016 ◽  
Vol 117 (10) ◽  
pp. 2315-2326 ◽  
Author(s):  
Teresita Padilla-Benavides ◽  
Cristina Velez-delValle ◽  
Meytha Marsch-Moreno ◽  
Federico Castro-Muñozledo ◽  
Walid Kuri-Harcuch
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipogenic Enzymes ◽  
Cytoplasmic Lipid Droplet ◽  
Lipid Droplet Formation

LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation

2010 ◽  
Vol 393 (2) ◽  
pp. 274-279 ◽  
Author(s):  
Masahiro Shibata ◽  
Kentaro Yoshimura ◽  
Hirosumi Tamura ◽  
Takashi Ueno ◽  
Taki Nishimura ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Cytoplasmic Lipid Droplet ◽  
Lipid Droplet Formation

scholarly journals Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2158 ◽  
Author(s):  
Xiaona Liu ◽  
Shifeng Wang ◽  
Zhisheng Wu ◽  
Zhaoyi Wang ◽  
Qiusheng Zheng ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Therapeutic Strategy ◽  
Laser Light ◽  
Imaging Study ◽  
Nonalcoholic Fatty Liver ◽  
Cytoplasmic Lipid Droplet ◽  
Uniform Dispersion ◽  
Ic50 Value ◽  
High Content Analysis

Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC50 value of 8.68 μM and 31.08 μM, respectively. The lipid droplet accumulation inhibition rate of 10 μM DS-lipo was above 90%, which was even superior to the effect of 30 μM DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD).

scholarly journals Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism

2007 ◽  
Vol 48 (7) ◽  
pp. 1463-1475 ◽  
Author(s):  
Tanya D. Russell ◽  
Carol A. Palmer ◽  
David J. Orlicky ◽  
Andreas Fischer ◽  
Michael C. Rudolph ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Epithelial Cells ◽  
Lipid Droplet ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Cytoplasmic Lipid Droplet

scholarly journals ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets

2021 ◽  
Vol 22 (3) ◽  
pp. 1311
Author(s):  
Franciele Lanzarini ◽  
Fernanda Alves Pereira ◽  
Janine de Camargo ◽  
Andressa Minozzo Oliveira ◽  
Katia Roberta Anacleto Belaz ◽  
...  
Keyword(s):  
Biological Control ◽  
Embryonic Development ◽  
Lipid Droplet ◽  
Cell Number ◽  
Protein Abundance ◽  
Droplet Deposition ◽  
Cytoplasmic Lipid Droplet ◽  
Technical Control ◽  
Lipid Species

Embryonic lipids are crucial for the formation of cellular membranes and dynamically participate in metabolic pathways. Cells can synthesize simple fatty acids, and the elongation of fatty acids facilitates the formation of complex lipids. The aim of this work was to investigate the involvement of the elongation of very long chain fatty acid enzyme 5 (ELOVL5) in embryonic development and lipid determination. Bovine embryos were produced in vitro using a standard protocol and randomly divided to receive one of three treatments at Day 4: morpholino (Mo) gene expression knockdown assay for ELOVL5 (ELOVL5-Mo), Mo antisense oligonucleotides for the thalassemic β-globulin human mRNA (technical control Mo), and placebo (biological control). The phenotypes of embryonic development, cell number, ELOVL5 protein abundance, lipid droplet deposits, and lipid fingerprint were investigated. No detrimental effects (p > 0.05) were observed on embryo development in terms of cleavage (59.4 ± 3.5%, 63.6 ± 4.1%, and 65.4 ± 2.2%), blastocyst production (31.3 ± 4.2%, 28.1 ± 4.9%, and 36.1 ± 2.1%), and blastocyst cell number (99.6 ± 7.7, 100.2 ± 6.2, 86.8 ± 5.6), respectively, for biological control, technical control Mo, and ELOVL5-Mo. ELOVL5 protein abundance and cytoplasmic lipid droplet deposition were increased (p < 0.05) in ELOVL5-Mo–derived blastocysts compared with the controls. However, seven lipid species, including phosphatidylcholines, phosphatidylethanolamines, and triacylglycerol, were downregulated in the ELOVL5-Mo–derived blastocysts compared with the biological control. Therefore, ELOVL5 is involved in the determination of embryonic lipid content and composition. Transient translational blockage of ELOVL5 reduced the expression of specific lipid species and promoted increased cytoplasmic lipid droplet deposition, but with no apparent deleterious effect on embryonic development and blastocyst cell number.

scholarly journals Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Clustering and Dispersion

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66837 ◽  
Author(s):  
David J. Orlicky ◽  
Jenifer Monks ◽  
Adrianne L. Stefanski ◽  
James L. McManaman
Keyword(s):  
Lipid Droplet ◽  
Cytoplasmic Lipid Droplet ◽  
Molecular Determinants
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