Lipogenic Enzymes Complexes and Cytoplasmic Lipid Droplet Formation During Adipogenesis

2016 ◽  
Vol 117 (10) ◽  
pp. 2315-2326 ◽  
Author(s):  
Teresita Padilla-Benavides ◽  
Cristina Velez-delValle ◽  
Meytha Marsch-Moreno ◽  
Federico Castro-Muñozledo ◽  
Walid Kuri-Harcuch
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipogenic Enzymes ◽  
Cytoplasmic Lipid Droplet ◽  
Lipid Droplet Formation

LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation

2010 ◽  
Vol 393 (2) ◽  
pp. 274-279 ◽  
Author(s):  
Masahiro Shibata ◽  
Kentaro Yoshimura ◽  
Hirosumi Tamura ◽  
Takashi Ueno ◽  
Taki Nishimura ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Cytoplasmic Lipid Droplet ◽  
Lipid Droplet Formation

ChemInform Abstract: Beauveriolides, Specific Inhibitors of Lipid Droplet Formation in Mouse Macrophages, Produced by Beauveria sp. FO-6979.

ChemInform ◽  
2010 ◽  
Vol 30 (27) ◽  
pp. no-no
Author(s):  
Ichiji Namatame ◽  
Hiroshi Tomoda ◽  
Shuyi Si ◽  
Yuichi Yamaguchi ◽  
Rokuro Masuma ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation ◽  
Mouse Macrophages ◽  
Specific Inhibitors

P0952 : Transient hepatic overexpression of the insuline-like growth factor 2 (IGF2) induces lipid droplet formation

2015 ◽  
Vol 62 ◽  
pp. S702-S703 ◽  
Author(s):  
S.M. Kessler ◽  
S. Laggai ◽  
E. Van Wonterghem ◽  
R.E. Vandenbroucke ◽  
M. Ogris ◽  
...  
Keyword(s):  
Growth Factor ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation

scholarly journals MicroRNA-122 Inhibits Lipid Droplet Formation and Hepatic Triglyceride Accumulation via Yin Yang 1

2017 ◽  
Vol 44 (4) ◽  
pp. 1651-1664 ◽  
Author(s):  
Guo-yi Wu ◽  
Chen Rui ◽  
Ji-qiao Chen ◽  
Eiketsu Sho ◽  
Shan-shan Zhan ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Luciferase Assay ◽  
Luciferase Reporter ◽  
Hepatic Triglyceride ◽  
Yin Yang 1 ◽  
Lipid Droplet Formation ◽  
Huh7 Cells ◽  
Yin Yang

Background/Aims: An increase in intracellular lipid droplet formation and hepatic triglyceride (TG) content usually results in nonalcoholic fatty liver disease. However, the mechanisms underlying the regulation of hepatic TG homeostasis remain unclear. Methods: Oil red O staining and TG measurement were performed to determine the lipid content. miRNA expression was evaluated by quantitative PCR. A luciferase assay was performed to validate the regulation of Yin Yang 1 (YY1) by microRNA (miR)-122. The effects of miR-122 expression on YY1 and its mechanisms involving the farnesoid X receptor and small heterodimer partner (FXR-SHP) pathway were evaluated by quantitative PCR and Western blot analyses. Results: miR-122 was downregulated in free fatty acid (FFA)-induced steatotic hepatocytes, and streptozotocin and high-fat diet (STZ-HFD) induced nonalcoholic steatohepatitis (NASH) in mice. Transfection of hepatocytes with miR-122 mimics before FFA induction inhibited lipid droplet formation and TG accumulation in vitro. These results were verified by overexpressing miR-122 in the livers of STZ-HFD-induced NASH mice. The 3’-untranslated region (3’UTR) of YY1 mRNA is predicted to contain an evolutionarily conserved miR-122 binding site. In silico searches, a luciferase reporter assay and quantitative PCR analysis confirmed that miR-122 directly bound to the YY1 3’UTR to negatively regulate YY1 mRNA in HepG2 and Huh7 cells. The (FXR-SHP) signaling axis, which is downstream of YY1, may play a key role in the mechanism of miR-122-regulated lipid homeostasis. YY1-FXR-SHP signaling, which is negatively regulated by FFA, was enhanced by miR-122 overexpression. This finding was also confirmed by overexpression of miR-122 in the livers of NASH mice. Conclusions: The present results indicate that miR-122 plays an important role in lipid (particularly TG) accumulation in the liver by reducing YY1 mRNA stability to upregulate FXR-SHP signaling.

scholarly journals Perilipin1 promotes unilocular lipid droplet formation through the activation of Fsp27 in adipocytes

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Zhiqi Sun ◽  
Jingyi Gong ◽  
Han Wu ◽  
Wenyi Xu ◽  
Lizhen Wu ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Droplet Formation ◽  
Lipid Droplet Formation
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