scholarly journals ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets

2021 ◽  
Vol 22 (3) ◽  
pp. 1311
Author(s):  
Franciele Lanzarini ◽  
Fernanda Alves Pereira ◽  
Janine de Camargo ◽  
Andressa Minozzo Oliveira ◽  
Katia Roberta Anacleto Belaz ◽  
...  
Keyword(s):  
Biological Control ◽  
Embryonic Development ◽  
Lipid Droplet ◽  
Cell Number ◽  
Protein Abundance ◽  
Droplet Deposition ◽  
Cytoplasmic Lipid Droplet ◽  
Technical Control ◽  
Lipid Species

Embryonic lipids are crucial for the formation of cellular membranes and dynamically participate in metabolic pathways. Cells can synthesize simple fatty acids, and the elongation of fatty acids facilitates the formation of complex lipids. The aim of this work was to investigate the involvement of the elongation of very long chain fatty acid enzyme 5 (ELOVL5) in embryonic development and lipid determination. Bovine embryos were produced in vitro using a standard protocol and randomly divided to receive one of three treatments at Day 4: morpholino (Mo) gene expression knockdown assay for ELOVL5 (ELOVL5-Mo), Mo antisense oligonucleotides for the thalassemic β-globulin human mRNA (technical control Mo), and placebo (biological control). The phenotypes of embryonic development, cell number, ELOVL5 protein abundance, lipid droplet deposits, and lipid fingerprint were investigated. No detrimental effects (p > 0.05) were observed on embryo development in terms of cleavage (59.4 ± 3.5%, 63.6 ± 4.1%, and 65.4 ± 2.2%), blastocyst production (31.3 ± 4.2%, 28.1 ± 4.9%, and 36.1 ± 2.1%), and blastocyst cell number (99.6 ± 7.7, 100.2 ± 6.2, 86.8 ± 5.6), respectively, for biological control, technical control Mo, and ELOVL5-Mo. ELOVL5 protein abundance and cytoplasmic lipid droplet deposition were increased (p < 0.05) in ELOVL5-Mo–derived blastocysts compared with the controls. However, seven lipid species, including phosphatidylcholines, phosphatidylethanolamines, and triacylglycerol, were downregulated in the ELOVL5-Mo–derived blastocysts compared with the biological control. Therefore, ELOVL5 is involved in the determination of embryonic lipid content and composition. Transient translational blockage of ELOVL5 reduced the expression of specific lipid species and promoted increased cytoplasmic lipid droplet deposition, but with no apparent deleterious effect on embryonic development and blastocyst cell number.

Recherches sur le rôle de la température dans la réalisation du phénotype chez des embryons de l'Amphibien Pleurodeles waltlii homozygotes pour la mutation thermosensible ac (ascite caudale)

Development ◽  
1975 ◽  
Vol 34 (1) ◽  
pp. 221-252
Author(s):  
Par Maria Fernandez ◽  
Jean-Claude Beetschen
Keyword(s):  
Embryonic Development ◽  
Developmental Stages ◽  
Fluid Collection ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Tail Bud ◽  
Dorsal Fin ◽  
Second Shift ◽  
Pleurodeles Waltlii

1. At the feeding stage (st. 38), a high percentage (79 %) of Pleurodeles homozygous ac/ac larvae show bent tails after a persistent ascitic blister in the dorsal part of the fin, when embryonic development occurred at 12°C; about only 25 % of them are affected by abdominal and pericardic ascites; about 40 % can feed and survive. The larval phenotype is very different when embryonic development occurred at 23 °C, in which case tail growth appears to be normal, but 95 % larvae die, due to ascitic fluid collection in the abdominal and heart regions, marked anaemia and microcephaly. 2. The exchange of posterior neural plates and dorso-lateral epidermis between normal and mutant neurulae has shown that the localization of the blister in the dorsal fin is not dependent on autonomous properties of the mutant dorsal tissues, but should be considered as resulting from general disturbances in the mutant organism. 3. Experiments were performed, involving a temperature shift from 12 to 23°C or 23 to 12°C, occurring at various developmental stages from the end of gastrulation (stage 13) to the stage of spontaneous embryonic muscle contractions (stage 26). When the temperature shift was applied after the end of neurulation (stage 21), the caudal phenotype was statistically similar to that of larvae which had been bred continuously at the first temperature. Thus temperature-sensitive phases can be characterized between neurula stages 15 and 18 (for a 12–23° shift) or 15 and 21 (for a 23–12° shift). Similarly, abdominal ascites can be induced when embryos are kept at 23 °C till stage 23 (early tail-bud) only, and occurs much less frequently when embryos are kept at 12°C till stage 23 and then transferred to 23°C. 4. It could be concluded from these experiments that the caudal mutant phenotype is already temperature-determined during neurulation, before stage 21. Nevertheless, double temperature-shift experiments showed that the second shift could modify the results which would be obtained if the first shift only occurred. Paradoxical results were obtained, more than 90 % of the tail phenotypes being of the ‘warm type’ when the embryos were first kept at 12°C, then shifted up to 23 °C between stages 22 and 26, and shifted down again to 12°C. Such a treatment markedly lowers the percentage of bent tails (‘cold type’) from the percentage which would occur if ac/ac embryos were constantly kept at 23 °C after stage 21, but this longer warm treatment is of no effect of itself as compared to the case when the whole development occurs at 12°C (bent tails are predominant in this latter case). Thus, whereas the early determination of the position of the caudal blister can be considered as a stable phenomenon under given temperature conditions, it is not irreversible. 5. As compared to cold-bred larvae, thrice as many completely anaemic larvae (66 %) were obtained from ac/ac embryos kept at 23 °C between stages 21 and 26; this offers an opportunity for the experimental study of this anaemia. 6. Implications of these results for further analysis of temperature-sensitive mutations in cold-blooded vertebrates are suggested.

Dihydroartemisinin reduced lipid droplet deposition by YAP1 to promote the anti-PD-1 effect in hepatocellular carcinoma

Phytomedicine ◽  
2021 ◽  
pp. 153913
Author(s):  
Liyuan Hao ◽  
Yinglin Guo ◽  
Qing Peng ◽  
Zhiqin Zhang ◽  
Jingmin Ji ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lipid Droplet ◽  
Droplet Deposition

P–060 Dose- dependent mitigation of lead acetate toxicity in males on embryo development in female mice

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
P Dolati ◽  
M J Zamiri ◽  
A Akhlaghi ◽  
Z Jahromi
Keyword(s):  
Adverse Effects ◽  
Embryonic Development ◽  
Embryo Development ◽  
Lead Acetate ◽  
Endocrine System ◽  
Cell Number ◽  
The Other ◽  
Control Group ◽  
Male Mice ◽  
Female Mice

Abstract Study question Does quercetin (75 or 100 mg/kg BW/day) co-administration with lead acetate to male mice affects embryonic development in female mice? Summary answer The low-dose quercetin (75 mg/kg BW/day) ameliorated the adverse effects of lead acetate on mouse embryogenesis. What is known already Lead causes male infertility by impacting on endocrine system and spermatogenesis, and may exert undesirable effects on the offspring. The currently approved treatment for lead poisoning is the use of chelating agents, which form an insoluble complex with lead and shield it from biological targets; thus, reducing its toxicity. One of the main mechanisms of lead-induced toxicity is oxidative stress, and it has been reported that natural antioxidants can reduce the heavy metals toxicity. The aim of the present study was to examine the protective effects of quercetin on the toxicity induced by lead acetate on the embryogenesis in mice. Study design, size, duration Sexually mature (eight-week-old) NMRI male mice (n = 24) were randomly divided into four groups (n = 6 per group) receiving (i) distilled water (control group); (ii) lead acetate (150 mg/kg BW/day) dissolved in deionized water (LA); (iii) lead acetate (150 mg/kg BW/day) + quercetin (75 mg/kg BW/day) (LQ75); (IV) lead acetate (150 mg/kg BW/day) + quercetin (100 mg/kg BW/day) (LQ100). Treatments were applied daily as oral gavages for one cycle of the seminiferous epithelium (35 days). Participants/materials, setting, methods At the end of treatment administration, the males were joined with super-ovulated females, and the retrieved zygotes were cultured for evaluation of the embryo development (at 2-cell, 4-cell, 8-cell, and blastocyst stages), and blastocyst cell number using differential staining (propidium iodide and bisbenzimide). After incubation of capacitated sperm with oocytes, an ultraviolet light microscope was used following 3 min incubation with 25 µg⁄mL bisbenzamide solution for fertilization assessment. Main results and the role of chance Lead acetate (LA) treatment of male mice decreased the 2-cell stage compared with the control group (P &gt; 0.05). There was no difference between control and LQ75, and between LA and LQ100. The other stages of embryonic development were not significantly affected by the treatment. Overall, early embryonic development in the control and LQ75 mice were better than LQ100 and LA mice. The number of cells in the trophectoderm and inner-cell mass were not affected by treatments. However, the total blastocyst cell number in the control was higher than in the other groups; there was no significant difference between LQ100, LQ75 and LA groups. Fertilization rate was not affected by the treatments (P &lt; 0.05). Quercetin acts as a potent antioxidant at low doses, but at high doses exerts a pro-oxidant action. According to previous reports, higher concentrations of quercetin increased apoptosis and necrosis while decreasing the activities of the antioxidant enzymes. Also, it has been suggested that quercetin might disrupt the endocrine system and interfere with Sertoli cell function and sperm motility. Limitations, reasons for caution A limitation of this study is narrow dose selection; more studies are needed to determine the effective dose of quercetin in ameliorating the lead toxicity. There are also side effects of lead-quercetin chelates such as metal redistribution, essential metal loss, accumulation and persistency in intracellular sites, and peroxidation. Wider implications of the findings: Lead administration adversely impacted on the embryogenesis; on the other hand, paternal quercetin co-administration somewhat ameliorated the adverse effects of lead on mice embryogenesis. Trial registration number Not applicable

Advantage of menadione-catalyzed chemiluminescent assay for the determination of viable mammalian cell number

2012 ◽  
Vol 421 (2) ◽  
pp. 428-432 ◽  
Author(s):  
Shiro Yamashoji ◽  
Naoko Yoshikawa ◽  
Masayuki Kirihara ◽  
Toshihiro Tsuneyoshi
Keyword(s):  
Mammalian Cell ◽  
Cell Number

scholarly journals Implementasi Teknologi Pengendalian Hayati Hama Kelapa Thosea monoloncha Meyrick dengan Nuclear Polyhedrosis Virus (NPV) [Biological Control Technology Implementation on Coconut Pest Thosea monoloncha Meyrick Using Nuclear Polyhedrosis Virus (NPV)]

Buletin Palma ◽  
2018 ◽  
Vol 19 (2) ◽  
pp. 89
Author(s):  
Fredy Lala ◽  
Andriko Noto Susanto ◽  
Meldy L.A Hosang ◽  
Deciyanto S
Keyword(s):  
Biological Control ◽  
Technology Implementation ◽  
Nuclear Polyhedrosis Virus ◽  
Control Technology ◽  
Light Damage ◽  
Severe Damage ◽  
Pest Population ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>The nettle caterpillars pest <em>Thosea monoloncha</em> (Limacodidae: Lepidoptera) has attacked the coconut plant in Tolonuo Island, North Halmahera district, North Maluku Province with the intensity of attacked varied from light  minor damage to severe damage. This research purpose is to control the population of nettle caterpillars <em>T. monoloncha </em>and the response of farmers to technology using <em>Nucleo Polyhedrosis Virus</em> (NPV). The study was conducted from December 2014 to November 2015. The stages of this study are consisted of (a) observation of pest population T. monoloncha, (b) determination of plant damage intensity and (c) farmerd response to control technology. The results showed that the application of NPV extract can decrease the population of coconut pest T. monoloncha, the intensity of the damage decrease from severe, heavy and moderate to light damage. In additon, it also increase the amount of frond and coconut production from 14 to 45 nuts per tree. Farmers' response to various aspects of technology varied from 63.5-97.5%.</p><p> </p><p align="center"><strong>ABSTRAK</strong></p><p align="center">Hama ulat api <em>Thosea monoloncha </em>(Limacodidae: Lepidoptera) telah menyerang tanaman kelapa di Pulau Tolonuo, Kabupaten Halmahera Utara, Provinsi Maluku Utara dengan intensitas kerusakan bervariasi dari ringan sampai sangat berat. Penelitian bertujuan untuk mengendalikan populasi hama ulat api <em>T. monoloncha </em>dan respon petani terhadap teknologi pemanfaatan <em>Nucleo Polyhedrosis Virus</em> (NPV).<strong> </strong>Penelitian dilaksanakan pada bulan Desember 2014 sampai November 2015. Hasil penelitian menunjukkan bahwa aplikasi ekstrak NPV dapat menurunkan populasi hama kelapa <em>T. monoloncha</em><em>,</em> menurunkan intesitas kerusakan dari kerusakan sangat berat, berat dan sedang menjadi kerusakan ringan, meningkatkan jumlah pelepah dan buah kelapa dari 14 butir menjadi 45 butir per pohon. Respon petani terhadap berbagai aspek teknologi berada pada kisaran 63,5-97,5%.  </p>

A quantitative approach to the detection of nuclear activity after differential damage to nucleus and cytoplasm in early development

Development ◽  
1968 ◽  
Vol 20 (1) ◽  
pp. 129-140
Author(s):  
A. A. Neyfakh ◽  
N. N. Rott
Keyword(s):  
Embryonic Development ◽  
Experimental Analysis ◽  
Main Method ◽  
Nuclear Activity ◽  
Complete Inactivation ◽  
Extent Of Damage ◽  
Sites Of Action ◽  
Nuclear Damage ◽  
Development Proceeds

For studying nucleo-cytoplasmic relations during development various selective influences on the nucleus and cytoplasm are widely used as the main method of experimental analysis. However, the application of such techniques presents difficulties both in obtaining evidence that shows the specificity of a nuclear or cytoplasmic effect by a chosen agent and in the quantitative evaluation of the extent of damage. In this paper a method is described for differentiating between nuclear and cytoplasmic sites of action of a given agent as well as for evaluating quantitatively the extent of nuclear damage. The method is based on the determination of the morphogenetic activity of nuclei at different stages of embryonic development. As has been previously shown, after complete inactivation of nuclei (for instance, by heavy doses of radiation) development proceeds up to the stages programmed for by the genetic cell apparatus (Neyfakh, 1959, 1964).

Biology of Host Range Testing for Biocontrol of Weeds

1990 ◽  
Vol 4 (3) ◽  
pp. 465-470 ◽  
Author(s):  
Gregory J. Weidemann ◽  
David O. Tebeest
Keyword(s):  
Biological Control ◽  
Host Range ◽  
Biological Control Agent ◽  
Full Range ◽  
Control Agent ◽  
Wild Plants ◽  
Weed Host ◽  
Close Phylogenetic Relationship ◽  
Related Plant

The determination of host range is an important component in developing a plant pathogen for use as a bioherbicide. The safety of non-target economic and wild plants must be assured before experimental release and commercial use. In contrast to other methods of weed control, the genetic variability and genetic stability of both the weed and the biological control agent must be considered. Schemes to determine host range generally assume a close phylogenetic relationship between the weed host and its co-evolved pathogens. Therefore, testing generally is based on inoculation of genetically related plant species and progresses to more distantly related species until the host range is circumscribed. Several potential weaknesses in these schemes will be illustrated with examples using specific biological control agents. Future tests must place greater emphasis on testing taxa representing the full range of genetic diversity within the biogeographic area of intended use.

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