Rescue from a two hit, high-throughput model of neurodegeneration with N-acetyl cysteine

2012 ◽  
Vol 61 (3) ◽  
pp. 356-368 ◽  
Author(s):  
Ajay S. Unnithan ◽  
Hailey J.H. Choi ◽  
Amanda M. Titler ◽  
Jessica M. Posimo ◽  
Rehana K. Leak
Keyword(s):  
High Throughput ◽  
Throughput Model ◽  
Acetyl Cysteine

A high-throughput model for fat graft assessment

2009 ◽  
Vol 41 (10) ◽  
pp. 738-744 ◽  
Author(s):  
Miguel A. Medina ◽  
John T. Nguyen ◽  
Michael M. McCormack ◽  
Mark A. Randolph ◽  
William G. Austen
Keyword(s):  
High Throughput ◽  
Fat Graft ◽  
Throughput Model

scholarly journals Toll‐DeficientDrosophilaFlies as a Fast, High‐Throughput Model for the Study of Antifungal Drug Efficacy against Invasive Aspergillosis andAspergillusVirulence

2005 ◽  
Vol 191 (7) ◽  
pp. 1188-1195 ◽  
Author(s):  
Michail S. Lionakis ◽  
Russell E. Lewis ◽  
Gregory S. May ◽  
Nathan P. Wiederhold ◽  
Nathaniel D. Albert ◽  
...  
Keyword(s):  
Invasive Aspergillosis ◽  
High Throughput ◽  
Drug Efficacy ◽  
Antifungal Drug ◽  
Throughput Model

Validation of visualized transgenic zebrafish as a high throughput model to assay bradycardia related cardio toxicity risk candidates

2012 ◽  
Vol 32 (10) ◽  
pp. 834-842 ◽  
Author(s):  
Dingsheng Wen ◽  
Aiming Liu ◽  
Feng Chen ◽  
Julin Yang ◽  
Renke Dai
Keyword(s):  
High Throughput ◽  
Transgenic Zebrafish ◽  
Throughput Model ◽  
Toxicity Risk

scholarly journals Drosophila melanogaster as a High-Throughput Model for Host–Microbiota Interactions

2017 ◽  
Vol 8 ◽  
Author(s):  
Mark Trinder ◽  
Brendan A. Daisley ◽  
Josh S. Dube ◽  
Gregor Reid
Keyword(s):  
Drosophila Melanogaster ◽  
High Throughput ◽  
Throughput Model

scholarly journals Dictyostelium discoideum as a Model System for Identification of Burkholderia pseudomallei Virulence Factors

2011 ◽  
Vol 79 (5) ◽  
pp. 2079-2088 ◽  
Author(s):  
Benjamin M. Hasselbring ◽  
Maharsh K. Patel ◽  
Mark A. Schell
Keyword(s):  
Dictyostelium Discoideum ◽  
High Throughput ◽  
Burkholderia Pseudomallei ◽  
Bacterial Pathogen ◽  
Model System ◽  
Virulence Determinants ◽  
Content Type ◽  
Throughput Model ◽  
Human Infections ◽  
High Throughput Assay

ABSTRACTBurkholderia pseudomalleiis an emerging bacterial pathogen and category B biothreat. Human infections withB. pseudomallei(called melioidosis) present as a range of manifestations, including acute septicemia and pneumonia. Although melioidosis can be fatal, little is known about the molecular basis ofB. pseudomalleipathogenicity, in part because of the lack of simple, genetically tractable eukaryotic models to facilitateen masseidentification of virulence determinants or explore host-pathogen interactions. Two assays, one high-throughput and one quantitative, were developed to monitor levels of resistance ofB. pseudomalleiand the closely related nearly avirulent speciesBurkholderia thailandensisto predation by the phagocytic amoebaDictyostelium discoideum. The quantitative assay showed that levels of resistance to, and survival within, amoeba by these bacteria and their known virulence mutants correlate well with their published levels of virulence in animals. Using the high-throughput assay, we screened a 1,500-memberB. thailandensistransposon mutant library and identified 13 genes involved in resistance to predation byD. discoideum. Orthologs of these genes were disrupted inB. pseudomallei, and nearly all mutants had similarly decreased resistance to predation byD. discoideum. For some mutants, decreased resistance also correlated with reduced survival in and cytotoxicity toward macrophages, as well as attenuated virulence in mice. These observations suggest that some factors required byB. pseudomalleifor resistance to environmental phagocytes also aid in resistance to phagocytic immune cells and contribute to disease in animals. Thus,D. discoideumprovides a novel, high-throughput model system for facilitating inquiry intoB. pseudomalleivirulence.

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