Validation of visualized transgenic zebrafish as a high throughput model to assay bradycardia related cardio toxicity risk candidates

2012 ◽  
Vol 32 (10) ◽  
pp. 834-842 ◽  
Author(s):  
Dingsheng Wen ◽  
Aiming Liu ◽  
Feng Chen ◽  
Julin Yang ◽  
Renke Dai
Keyword(s):  
High Throughput ◽  
Transgenic Zebrafish ◽  
Throughput Model ◽  
Toxicity Risk

A high-throughput model for fat graft assessment

2009 ◽  
Vol 41 (10) ◽  
pp. 738-744 ◽  
Author(s):  
Miguel A. Medina ◽  
John T. Nguyen ◽  
Michael M. McCormack ◽  
Mark A. Randolph ◽  
William G. Austen
Keyword(s):  
High Throughput ◽  
Fat Graft ◽  
Throughput Model

scholarly journals Toll‐DeficientDrosophilaFlies as a Fast, High‐Throughput Model for the Study of Antifungal Drug Efficacy against Invasive Aspergillosis andAspergillusVirulence

2005 ◽  
Vol 191 (7) ◽  
pp. 1188-1195 ◽  
Author(s):  
Michail S. Lionakis ◽  
Russell E. Lewis ◽  
Gregory S. May ◽  
Nathan P. Wiederhold ◽  
Nathaniel D. Albert ◽  
...  
Keyword(s):  
Invasive Aspergillosis ◽  
High Throughput ◽  
Drug Efficacy ◽  
Antifungal Drug ◽  
Throughput Model

scholarly journals A calibrated optogenetic toolbox of stable zebrafish opsin lines

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Paride Antinucci ◽  
Adna Dumitrescu ◽  
Charlotte Deleuze ◽  
Holly J Morley ◽  
Kristie Leung ◽  
...  
Keyword(s):  
High Throughput ◽  
Neural Activity ◽  
Ion Selectivity ◽  
Transgenic Zebrafish ◽  
Substantial Variation ◽  
Opsin Expression ◽  
Electrophysiological Recordings ◽  
Subcellular Resolution ◽  
Kinetics Of

Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.

scholarly journals Investigating the application of a nitroreductase-expressing transgenic zebrafish line for high-throughput toxicity testing

2017 ◽  
Vol 4 ◽  
pp. 202-210 ◽  
Author(s):  
Anna C. Chlebowski ◽  
Jane K. La Du ◽  
Lisa Truong ◽  
Staci L. Massey Simonich ◽  
Robert L. Tanguay
Keyword(s):  
High Throughput ◽  
Toxicity Testing ◽  
Transgenic Zebrafish

scholarly journals Drosophila melanogaster as a High-Throughput Model for Host–Microbiota Interactions

2017 ◽  
Vol 8 ◽  
Author(s):  
Mark Trinder ◽  
Brendan A. Daisley ◽  
Josh S. Dube ◽  
Gregor Reid
Keyword(s):  
Drosophila Melanogaster ◽  
High Throughput ◽  
Throughput Model

Rescue from a two hit, high-throughput model of neurodegeneration with N-acetyl cysteine

2012 ◽  
Vol 61 (3) ◽  
pp. 356-368 ◽  
Author(s):  
Ajay S. Unnithan ◽  
Hailey J.H. Choi ◽  
Amanda M. Titler ◽  
Jessica M. Posimo ◽  
Rehana K. Leak
Keyword(s):  
High Throughput ◽  
Throughput Model ◽  
Acetyl Cysteine
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