scholarly journals A high-throughput model of post-traumatic osteoarthritis using engineered cartilage tissue analogs

2014 ◽  
Vol 22 (9) ◽  
pp. 1282-1290 ◽  
Author(s):  
B. Mohanraj ◽  
G.R. Meloni ◽  
R.L. Mauck ◽  
G.R. Dodge
Keyword(s):  
High Throughput ◽  
Cartilage Tissue ◽  
Throughput Model ◽  
Post Traumatic ◽  
Engineered Cartilage

A high-throughput model for fat graft assessment

2009 ◽  
Vol 41 (10) ◽  
pp. 738-744 ◽  
Author(s):  
Miguel A. Medina ◽  
John T. Nguyen ◽  
Michael M. McCormack ◽  
Mark A. Randolph ◽  
William G. Austen
Keyword(s):  
High Throughput ◽  
Fat Graft ◽  
Throughput Model

Tissue-Engineered Cartilage in Three-Dimensional Cultured Chondrocytes by Ultrasound Stimulation and Collagen Sponge as Scaffold

2009 ◽  
Author(s):  
Toshihiko Shiraishi ◽  
Ietomo Matsunaga ◽  
Shin Morishita ◽  
Ryohei Takeuchi ◽  
Tomoyuki Saito ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Three Dimensional ◽  
Collagen Sponge ◽  
Cartilage Tissue ◽  
Mechanical Environment ◽  
Ultrasound Stimulation ◽  
Scaffold Structure ◽  
Matrix Production ◽  
Engineered Cartilage ◽  
Cultured Chondrocytes

This paper describes the effects of ultrasound stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen sponges as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis). It has been shown that cell proliferation and matrix production can be facilitated by considering the mechanical environment of the cultured chondrocytes and the mechanical properties of the scaffold structure used in the culture and of the stimulation used.

scholarly journals An Instrumented Bioreactor for Mechanical Stimulation and Real-Time, Nondestructive Evaluation of Engineered Cartilage Tissue

2012 ◽  
Vol 6 (2) ◽  
Author(s):  
Jenni R. Popp ◽  
Justine J. Roberts ◽  
Doug V. Gallagher ◽  
Kristi S. Anseth ◽  
Stephanie J. Bryant ◽  
...  
Keyword(s):  
Nondestructive Evaluation ◽  
Mechanical Stimulation ◽  
Ultrasonic Transducer ◽  
Testing Machine ◽  
Cartilage Tissue ◽  
Matrix Deposition ◽  
Intermittent Compression ◽  
Engineered Tissue ◽  
Engineered Cartilage

Mechanical stimulation is essential for chondrocyte metabolism and cartilage matrix deposition. Traditional methods for evaluating developing tissue in vitro are destructive, time consuming, and expensive. Nondestructive evaluation of engineered tissue is promising for the development of replacement tissues. Here we present a novel instrumented bioreactor for dynamic mechanical stimulation and nondestructive evaluation of tissue mechanical properties and extracellular matrix (ECM) content. The bioreactor is instrumented with a video microscope and load cells in each well to measure tissue stiffness and an ultrasonic transducer for evaluating ECM content. Chondrocyte-laden hydrogel constructs were placed in the bioreactor and subjected to dynamic intermittent compression at 1 Hz and 10% strain for 1 h, twice per day for 7 days. Compressive modulus of the constructs, measured online in the bioreactor and offline on a mechanical testing machine, did not significantly change over time. Deposition of sulfated glycosaminoglycan (sGAG) increased significantly after 7 days, independent of loading. Furthermore, the relative reflection amplitude of the loaded constructs decreased significantly after 7 days, consistent with an increase in sGAG content. This preliminary work with our novel bioreactor demonstrates its capabilities for dynamic culture and nondestructive evaluation.

scholarly journals Discovery of potential therapeutics for post-traumatic osteoarthritis using a high throughput mechanical injury platform

2019 ◽  
Vol 27 ◽  
pp. S509
Author(s):  
J. Cresta ◽  
F. Yousefi ◽  
E. Rabut ◽  
B. Mohanraj ◽  
R.L. Mauck ◽  
...  
Keyword(s):  
High Throughput ◽  
Mechanical Injury ◽  
Post Traumatic ◽  
Potential Therapeutics

scholarly journals Challenges in Fabrication of Tissue-Engineered Cartilage with Correct Cellular Colonization and Extracellular Matrix Assembly

2018 ◽  
Vol 19 (9) ◽  
pp. 2700 ◽  
Author(s):  
Mikko Lammi ◽  
Juha Piltti ◽  
Juha Prittinen ◽  
Chengjuan Qu
Keyword(s):  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Pore Size ◽  
Cartilage Tissue ◽  
Cellular Interactions ◽  
Matrix Assembly ◽  
Small Pore ◽  
Culture Models ◽  
Open Question ◽  
Engineered Cartilage

A correct articular cartilage ultrastructure regarding its structural components and cellularity is important for appropriate performance of tissue-engineered articular cartilage. Various scaffold-based, as well as scaffold-free, culture models have been under development to manufacture functional cartilage tissue. Even decellularized tissues have been considered as a potential choice for cellular seeding and tissue fabrication. Pore size, interconnectivity, and functionalization of the scaffold architecture can be varied. Increased mechanical function requires a dense scaffold, which also easily restricts cellular access within the scaffold at seeding. High pore size enhances nutrient transport, while small pore size improves cellular interactions and scaffold resorption. In scaffold-free cultures, the cells assemble the tissue completely by themselves; in optimized cultures, they should be able to fabricate native-like tissue. Decellularized cartilage has a native ultrastructure, although it is a challenge to obtain proper cellular colonization during cell seeding. Bioprinting can, in principle, provide the tissue with correct cellularity and extracellular matrix content, although it is still an open question as to how the correct molecular interaction and structure of extracellular matrix could be achieved. These are challenges facing the ongoing efforts to manufacture optimal articular cartilage.

The Response of Tissue Engineered Cartilage to the Temporal Application of Transforming and Insulin-Like Growth Factors

2007 ◽  
Author(s):  
Christopher J. O’Conor ◽  
Kenneth W. Ng ◽  
Lindsay E. Kugler ◽  
Gerard A. Ateshian ◽  
Clark T. Hung
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Mechanical Stimuli ◽  
Tissue Development ◽  
Engineering Research ◽  
Engineered Cartilage

Agarose has been used as an experimental scaffold for cartilage tissue engineering research due to its biocompatibility with chondrocytes, support of cartilage tissue development, and ability to transmit mechanical stimuli [1–3]. Tissue engineering studies have demonstrated that the temporal application of transforming growth factor (TGF) β3 for only 2 weeks elicits rapid tissue development that results in mechanical properties approaching native values [4]. However, it is not known whether this response to a 2-week exposure to growth factors is unique to TGF-β3. Therefore, the present study characterizes the response of tissue engineered cartilage to the temporal application of the anabolic growth factors TGF-β1, TGF-β3, and insulin-like growth factor I (IGF-I).

scholarly journals Coating Medpor® Implant with Tissue-Engineered Elastic Cartilage

2020 ◽  
Vol 11 (2) ◽  
pp. 34
Author(s):  
Dong Joon Lee ◽  
Jane Kwon ◽  
Yong-Il Kim ◽  
Yong Hoon Kwon ◽  
Samuel Min ◽  
...  
Keyword(s):  
Histological Section ◽  
Medical Application ◽  
Neck Surgery ◽  
Enzymatic Digestion ◽  
Cartilage Tissue ◽  
Plga Scaffold ◽  
Biodegradable Poly ◽  
Engineering Strategy ◽  
Novel Method ◽  
Engineered Cartilage

Inert biomaterials used for auricular reconstruction, which is one of the most challenging and diverse tasks in craniofacial or head and neck surgery, often cause problems such as capsule formation, infection, and skin extrusion. To solve these problems, scaffold consisting of inert biomaterial, high-density polyethylene (Medpor®) encapsulated with neocartilage, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) was created using a tissue engineering strategy. PLGA scaffold without Medpor® was created to serve as the control. Scaffolds were vacuum-seeded with rabbit chondrocytes, freshly isolated from the ear by enzymatic digestion. Then, cell-seeded scaffolds were implanted subcutaneously in the dorsal pockets of nude mice. After 12 weeks, explants were analyzed by histological, biochemical, and mechanical evaluations. Although the PLGA group resulted in neocartilage formation, the PLGA–Medpor® group demonstrated improved outcome with the formation of well-surrounded cartilage around the implants with higher mechanical strength than the PLGA group, indicating that Medpor® has an influence on the structural strength of engineered cartilage. The presence of collagen and elastin fibers was evident in the histological section in both groups. These results demonstrated a novel method of coating implant material with engineered cartilage to overcome the limitations of using biodegradable scaffold in cartilage tissue regeneration. By utilizing the patient’s own chondrocytes, our proposed method may broaden the choice of implant materials while minimizing side effects and immune reaction for the future medical application.

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