Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing

2016 ◽  
Vol 25 ◽  
pp. 38-49 ◽  
Author(s):  
José Miguel Alvarez-Suarez ◽  
Francesca Giampieri ◽  
Mario Cordero ◽  
Massimiliano Gasparrini ◽  
Tamara Yuliet Forbes-Hernández ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oxidative Damage ◽  
Mitochondrial Function ◽  
Antioxidant Response ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Manuka Honey

scholarly journals Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeong Yu Lee ◽  
Dae-Jin Min ◽  
Wanil Kim ◽  
Bum-Ho Bin ◽  
Kyuhan Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Intensity ◽  
Mapk Pathway ◽  
Dermal Fibroblasts ◽  
Ultrasound Treatment ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Short Term ◽  
High Intensity Ultrasound ◽  
Extracellular Matrix Components

AbstractInspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.

scholarly journals Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

2014 ◽  
Vol 320 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Nina Tandon ◽  
Elisa Cimetta ◽  
Aranzazu Villasante ◽  
Nicolette Kupferstein ◽  
Michael D. Southall ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

2015 ◽  
Vol 34 (2) ◽  
pp. 96
Author(s):  
Linda Yuliati ◽  
Etik Mardliyati ◽  
Kusmarinah Bramono ◽  
Hans Joachim Freisleben
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Retinoic Acid ◽  
Collagen Synthesis ◽  
Active Agent ◽  
Dermal Fibroblasts ◽  
Type Iii Collagen ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Type Iii

Background<br />Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast.<br /><br />Methods<br />Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups. <br /><br />Results<br />Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p&lt;0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p&lt;0.05). <br /><br />Conclusion<br />Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

Studies on Wound Healing: Effects of Calcium D-Pantothenate on the Migration, Proliferation and Protein Synthesis of Human Dermal Fibroblasts in Culture

1999 ◽  
Vol 69 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Weimann ◽  
Hermann
Keyword(s):  
Wound Healing ◽  
Protein Synthesis ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Wound Healing Process ◽  
Human Dermal Fibroblasts ◽  
The Mean ◽  
Cell Densities ◽  
Migration Of Cells ◽  
Wounded Area

The effect of calcium D-pantothenate on the migration, proliferation and protein synthesis of human dermal fibroblasts from three different donors was investigated. The migration of cells into a wounded area was dose-dependently stimulated by Ca D-pantothenate. The number of cells that migrated across the edge of the wound increased from 32 ± 7 cells/ mm without Ca D-pantothenate to 76 ± 2 cells/ mm with 100 mg/ml Ca D-pantothenate. Moreover, the mean migration distance per cell increased from 0.23 ± 0.05 mm to 0.33 ± 0.02 mm. The mean migration speed was calculated to be 10.5 mm/hour without and 15 mm/hour with Ca D-pantothenate. Cell proliferation was also dose-dependently stimulated. The final cell densities were 1.2 to 1.6-fold higher in cultures containing 100 mg/ml Ca D-pantothenate. The protein synthesis was modulated, since two unidentified proteins were more strongly expressed in pantothenate supplemented cultures. In conclusion, Ca D-pantothenate accelerates the wound healing process by increasing the number of migrating cells, their distance and hence their speed. In addition, cell division is increased and the protein synthesis changed. These results suggest that higher quantities of pantothenate are locally required to enhance wound healing.

scholarly journals The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality

2017 ◽  
Vol 8 (9) ◽  
pp. 3250-3258 ◽  
Author(s):  
José M. Alvarez-Suarez ◽  
Francesca Giampieri ◽  
Massimiliano Gasparrini ◽  
Luca Mazzoni ◽  
Celestino Santos-Buelga ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Antioxidant Enzyme ◽  
Bioactive Compounds ◽  
Antioxidant Enzyme Activity ◽  
Dermal Fibroblasts ◽  
Natural Source ◽  
Human Dermal Fibroblasts ◽  
Malpighia Emarginata

Acerola fruits are shown as an important natural source of bioactive compounds with beneficial properties for health.

In vitro Proliferation and Wound Healing Effects of Narcissus tazetta L. Bulb on Primary Human Dermal Fibroblasts

2018 ◽  
Vol 20 (6) ◽  
pp. 1-11 ◽  
Author(s):  
Maryam Rameshk ◽  
Fariba Sharififar ◽  
Mitra Mehrabani ◽  
Abbas Pardakhty ◽  
Alireza Farsinejad
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Narcissus Tazetta ◽  
In Vitro Proliferation

scholarly journals Prevention of UVA-Induced Oxidative Damage in Human Dermal Fibroblasts by New UV Filters, Assessed Using a Novel In Vitro Experimental System

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83401 ◽  
Author(s):  
Francesca Brugè ◽  
Luca Tiano ◽  
Paola Astolfi ◽  
Monica Emanuelli ◽  
Elisabetta Damiani
Keyword(s):  
Oxidative Damage ◽  
Experimental System ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Uv Filters
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