In vitro Proliferation and Wound Healing Effects of Narcissus tazetta L. Bulb on Primary Human Dermal Fibroblasts

2018 ◽  
Vol 20 (6) ◽  
pp. 1-11 ◽  
Author(s):  
Maryam Rameshk ◽  
Fariba Sharififar ◽  
Mitra Mehrabani ◽  
Abbas Pardakhty ◽  
Alireza Farsinejad
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Narcissus Tazetta ◽  
In Vitro Proliferation

scholarly journals Interaction of Gold Nanorods with Human Dermal Fibroblasts: Cytotoxicity, Cellular Uptake, and Wound Healing

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1131
Author(s):  
Nouf N. Mahmoud ◽  
Lubna M. Al-Kharabsheh ◽  
Enam A. Khalil ◽  
Rana Abu-Dahab
Keyword(s):  
Wound Healing ◽  
Cellular Uptake ◽  
Gold Nanorods ◽  
Healing Rate ◽  
Scratch Test ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Poly Ethylene ◽  
Wound Healing Rate

Herein, the cytotoxicity, cellular uptake and wound healing of human dermal fibroblasts were investigated upon treatment with gold nanorods (GNR) decorated with different ligands. Neutral and cationic poly ethylene glycol (PEG)-decorated GNR demonstrated the least cytotoxicity and cellular internalization, while anionic- and bovine serum albumin (BSA)-coated GNR revealed significant cytotoxicity and cellular uptake into human dermal fibroblasts. The cell scratch test demonstrated that neutral, cationic PEGylated GNR and anionic-decorated GNR have accelerated the wound healing rate in vitro after 24 h of incubation with scratched human dermal fibroblasts compared to control, while there was a drastic retardation of wound healing rate of scratched fibroblasts upon exposure to BSA-GNR accompanied with a significant release of the inflammatory cytokine; interlukin-1β (IL-1β). The cytotoxicity of GNR against the dermal cells and their ability to enhance the wound healing in vitro are greatly linked to their surface modifications.

Wound Healing Activity of Carbonized Rice Husk

2012 ◽  
Vol 602-604 ◽  
pp. 1196-1199
Author(s):  
Nuraly Akimbekov ◽  
Zulhair A. Mansurov ◽  
J. Jandosov ◽  
Ilya E. Digel ◽  
Mathias Gossmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rice Husk ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Rat Skin ◽  
Cellular Behavior ◽  
Proliferation In Vitro ◽  
Wound Healing Activity

The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.

scholarly journals Modulation of Skin Inflammatory Response by Active Components of Silymarin

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 123 ◽  
Author(s):  
Jana Juráňová ◽  
Juliette Aury-Landas ◽  
Karim Boumediene ◽  
Catherine Baugé ◽  
David Biedermann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Prolonged Exposure ◽  
Dermal Fibroblasts ◽  
Enzyme Linked Immunosorbent Assay ◽  
Activator Protein 1 ◽  
Human Dermal Fibroblasts ◽  
Active Components ◽  
Mobility Shift ◽  
Anti Inflammatory

In this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with persistent inflammation, we studied their anti-inflammatory activity against inflammation induced by bacterial lipopolysaccharide (LPS). We investigated the regulation of crucial pro-inflammatory transcription factors—nuclear factor kappa-B (NF-κB) and activator protein 1 (AP-1)—as well as the expression of downstream inflammatory targets by Western blotting, real-time PCR (RT-PCR), electrophoretic mobility shift assay (EMSA), and/or enzyme-linked immunosorbent assay (ELISA) in vitro using primary normal human dermal fibroblasts (NHDF). We demonstrated the greater ability of DHS to modulate the pro-inflammatory cytokines production via the NF-κB and AP-1 signaling pathways when compared to other tested substances. The prolonged exposure of LPS-challenged human dermal fibroblasts to DHS had both beneficial and detrimental consequences. DHS diminished interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion but induced the significant upregulation of IL-8 mRNA associated with NF-κB and AP-1 activation. The observed conflicting results may compromise the main expected benefit, which is the acceleration of the healing of the wound via a diminished inflammation.

scholarly journals Aristolochia bracteolata Enhances Wound Healing in vitro through Anti-inflammatory and Proliferative Effect on Human Dermal Fibroblasts and Keratinocytes

2017 ◽  
Vol 9 (6s) ◽  
pp. s129-s136 ◽  
Author(s):  
Dinesh Murugan Girija ◽  
Mangathayaru Kalachaveedu ◽  
Rajasekaran Subbarayan ◽  
Preethi Jenifer ◽  
Suresh Ranga Rao
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Proliferative Effect ◽  
Anti Inflammatory ◽  
Aristolochia Bracteolata

scholarly journals Effect of Bioactive Phytochemicals from Phlomis viscosa Poiret on Wound Healing

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Ludmila Yarmolinsky ◽  
Arie Budovsky ◽  
Leonid Yarmolinsky ◽  
Boris Khalfin ◽  
Vladimir Glukhman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Beneficial Effect ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
In Vivo Models ◽  
Bioactive Phytochemicals ◽  
Pro Inflammatory Cytokines

Phlomis viscosa Poiret is an evergreen shrub growing in Israel, Turkey, Lebanon, and Syria with acknowledged pro-wound healing (WH) properties. In this study, we evaluated the pro-WH potential of selected compounds found in this plant. Among the pro-WH compounds (identified by us) was a combination of three chemicals—diosmin, 1-octen-3-ol, and himachala-2,4-diene which enhanced WH significantly both in in vitro and in vivo models. The determined phytochemicals combination could be used for the treatment of chronic wounds. The effect of the extracts, diosmin, 1-octen-3-ol on the secretion of pro-inflammatory cytokines, IL-6 (A) and IL-8 (B) by human dermal fibroblasts was significant (p < 0.001). In addition, the beneficial effect of extracts of P. viscosa and its phytochemicals on WH was evidenced by inhibiting the growth of several WH delaying microorganisms.

Wound-healing plants from TCM: in vitro investigations on selected TCM plants and their influence on human dermal fibroblasts and keratinocytes

Fitoterapia ◽  
2013 ◽  
Vol 84 ◽  
pp. 308-317 ◽  
Author(s):  
Ruxi Wang ◽  
Matthias Lechtenberg ◽  
Jandirk Sendker ◽  
Frank Petereit ◽  
Alexandra Deters ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals Effects of cardiotonic steroids on dermal collagen synthesis and wound healing

2008 ◽  
Vol 105 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Nasser El-Okdi ◽  
Sleiman Smaili ◽  
Vanamala Raju ◽  
Amjad Shidyak ◽  
Shalini Gupta ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Synthesis ◽  
Cardiac Fibroblasts ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Accelerate Wound Healing ◽  
Proline Incorporation ◽  
Dermal Collagen

We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na-K-ATPase pathway (Elkareh et al. Hypertension 49: 215–224, 2007). In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid marinobufagenin in a dose- and time-dependent fashion. An eightfold increase in collagen synthesis was noted when cells were exposed to 10 nM marinobufagenin for 24 h ( P < 0.01). Similar increases in proline incorporation were seen following treatment with digoxin, ouabain, and marinobufagenin (10 nM × 24 h, all results P < 0.01 vs. control). The coadministration of the Src inhibitor PP2 or N-acetylcysteine completely prevented collagen stimulation by marinobufagenin. Next, we examined the effect of digoxin, ouabain, and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model. Olive oil was chosen as the digoxin carrier because of a favorable partition coefficient observed for labeled digoxin with saline. This application significantly accelerated in vivo wound healing in rats wounded with an 8-mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both P < 0.01). The data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, as could potentially be exploited to accelerate wound healing.

scholarly journals Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeong Yu Lee ◽  
Dae-Jin Min ◽  
Wanil Kim ◽  
Bum-Ho Bin ◽  
Kyuhan Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
High Intensity ◽  
Mapk Pathway ◽  
Dermal Fibroblasts ◽  
Ultrasound Treatment ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Short Term ◽  
High Intensity Ultrasound ◽  
Extracellular Matrix Components

AbstractInspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.

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