Optimization of extraction methods for determination of the raffinose family oligosaccharides in leguminous vine peas (Pisum sativum L.) and effects of blanching

2007 ◽  
Vol 20 (1) ◽  
pp. 13-18 ◽  
Author(s):  
J. Ekvall ◽  
R. Stegmark ◽  
M. Nyman
Planta ◽  
1984 ◽  
Vol 161 (4) ◽  
pp. 302-307 ◽  
Author(s):  
C. L. Díaz ◽  
P. Lems-van Kan ◽  
I. A. M. Van der Schaal ◽  
J. W. Kijne

2010 ◽  
Vol 20 (4) ◽  
pp. 213-221 ◽  
Author(s):  
Lesław B. Lahuta ◽  
Wojciech Święcicki ◽  
Tomasz Dzik ◽  
Ryszard J. Górecki ◽  
Marcin Horbowicz

AbstractFeeding stem–leaf–pod explants with d-chiro-inositol and d-pinitol was used as a method to modify α-d-galactosides in developing pea (Pisum sativum) seeds. Four genotypes differing in the composition of raffinose, stachyose and verbascose (raffinose family oligosaccharides or RFOs) in seeds – high RFOs (cv. Tiny), low RFOs (SZD175) and low verbascose (cv. Hubal and cv. Wt 506) – were studied. Although seeds of all examined pea lines were able to take up both d-chiro-inositol and d-pinitol, only d-chiro-inositol was effectively converted into its galactosides: mainly fagopyritol B1 (O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol) and fagopyritol B2 (O-α-d-galactopyranosyl-(1 → 6)-O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol). In seeds of pea lines naturally containing low levels of verbascose (cv. Hubal) and low RFOs (SZD175), the enhanced accumulation of fagopyritols depressed the RFO level by c. 64 and 20%, respectively. Moreover, in both genotypes, about 25 and 30% of total galactose bound in α-d-galactosides occurred in fagopyritols. d-Pinitol present in the pea seeds was converted into monogalactosides, but their accumulation was several-fold lower than that of fagopyritols and did not significantly influence the accumulation of RFOs. Pea seeds with the composition of soluble carbohydrates modified by feeding with either of the cyclitols were able to complete germination.


1982 ◽  
Vol 50 (6) ◽  
pp. 757-762 ◽  
Author(s):  
M. A. RANA ◽  
P. B. GAHAN

1994 ◽  
Vol 74 (4) ◽  
pp. 853-855 ◽  
Author(s):  
B. N. Kaiser ◽  
B. J. Shelp ◽  
P. Thumfort ◽  
D. B. Layzell

Two O2 ramping techniques (linear versus exponential) were used to investigate the response of H2 evolution from intact nodules of soybean (Glycine max (L.) Merr. 'Maple Arrow'), stem-girdled soybean, pea (Pisum sativum L. 'Juneau'), and common bean (Phaseolus vulgaris L. 'Ex Rico 23') to increasing O2 concentrations from 20 to 100% over a 30-min period. The data indicate symbiosis-specific responses to the two ramps, and possible implications for determination of O2 limitation of N2 fixation. Key words: Hydrogen evolution, legume, nitrogen fixation


Author(s):  
Clara L. Díaz ◽  
Ton J. van Driel ◽  
Ineke A. M. van der Schaal ◽  
Jan W. Kijne

2017 ◽  
Vol 23 (4) ◽  
Author(s):  
Milomir Blagojević ◽  
Nenad Đorđević ◽  
Bora D ◽  
Jordan Marković ◽  
Tanja Vasić ◽  
...  

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