Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera

2009 ◽  
Vol 47 (6) ◽  
pp. 1109-1116 ◽  
Author(s):  
Brahma N. Singh ◽  
B.R. Singh ◽  
R.L. Singh ◽  
D. Prakash ◽  
R. Dhakarey ◽  
...  
Keyword(s):  
Dna Damage ◽  
Quorum Sensing ◽  
Moringa Oleifera ◽  
Oxidative Dna Damage ◽  
Protective Activity ◽  
Dna Damage Protective

scholarly journals OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

2018 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Andab Pant
Keyword(s):  
Dna Damage ◽  
Hepg2 Cells ◽  
Oxidative Dna Damage ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Protective Activity ◽  
Trypan Blue Exclusion ◽  
Dna Damage Protective

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH•) and hepatoprotective activity against CCl4 induced toxicity in HepG2 liver cells.Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton’s reagent. Protection of human liver-derived HepG2 cells against CCl4 induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 µmol/ml of CCl4 for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 µg/ml.Results: The low dose (0.001-0.1 µg/ml) of leaf and bark has an effect on the percentage viability (101±4.04) and 97±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99±2.89 and 103±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica. Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl4 exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

Evaluation of the DNA damage protective activity of the germinated peanut (Arachis hypogaea) in relation to antioxidant and anti-inflammatory activity

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 259-268 ◽  
Author(s):  
Apinun Limmongkon ◽  
Jintana Pankam ◽  
Thapakorn Somboon ◽  
Pakwuan Wongshaya ◽  
Phatcharaporn Nopprang
Keyword(s):  
Dna Damage ◽  
Arachis Hypogaea ◽  
Inflammatory Activity ◽  
Protective Activity ◽  
Anti Inflammatory ◽  
Dna Damage Protective

Modulatory effects of Moringa oleifera extracts against hydrogen peroxide-induced cytotoxicity and oxidative damage

2010 ◽  
Vol 30 (9) ◽  
pp. 1359-1368 ◽  
Author(s):  
S Sreelatha ◽  
PR Padma
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Moringa Oleifera ◽  
Oxidative Dna Damage ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Kb Cells ◽  
Leaf Extracts

Studies have demonstrated that the induction of oxidative stress may be involved in oxidative DNA damage. The present study examined and assessed the hydrogen peroxide (H2O2)-mediated DNA damage in human tumor KB cells and also assessed the ability of Moringa oleifera leaf extracts to inhibit the oxidative damage. H2O2 imposed a stress on the membrane lipids which was quantified by the extent of thiobarbituric acid reactive substances (TBARS) formed. The leaf extracts caused a very significant inhibition of the extent of LPO formation and enhanced the activity of antioxidative enzymes such as superoxide dismutase (SOD) and catalase (CAT) in KB cells. The comet assay was employed to study the DNA damage and its inhibition by the leaf extracts. H2O2 caused a significant increase in the number of cells bearing comets, resulting in significant DNA damage. The leaf extracts significantly reduced the incidence of comets in the oxidant stressed cells. The extent of cytotoxicity of H2O2 in the presence and the absence of leaf extracts studied in KB tumor cells by the MTT assay showed that H2O2 caused a marked decrease in the viability of KB cells where as the leaf extracts effectively increased the viability of assaulted KB cells. The observed cytoprotective activity is probably due to the antioxidant properties of its constituents, mainly phenolics. Total phenolics showed higher correlation with antioxidant activity. The leaf extracts showed higher antioxidant activity than the reference compound. These results suggest that the inhibition by the leaf extracts on oxidative DNA damage could be attributed to their free radical scavenging activities and the effect evidenced in KB cells can be in part correlated to a modulation of redox-sensitive mechanisms.

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