Antioxidant, oxidative DNA damage protective and antimicrobial activities of the plantTrigonella foenum-graecum

2014 ◽  
Vol 94 (12) ◽  
pp. 2497-2504 ◽  
Author(s):  
Pankaj Singh ◽  
Shri P Vishwakarma ◽  
Ram L Singh
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Antimicrobial Activities ◽  
Dna Damage Protective

scholarly journals OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

2018 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Andab Pant
Keyword(s):  
Dna Damage ◽  
Hepg2 Cells ◽  
Oxidative Dna Damage ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Protective Activity ◽  
Trypan Blue Exclusion ◽  
Dna Damage Protective

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH•) and hepatoprotective activity against CCl4 induced toxicity in HepG2 liver cells.Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton’s reagent. Protection of human liver-derived HepG2 cells against CCl4 induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 µmol/ml of CCl4 for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 µg/ml.Results: The low dose (0.001-0.1 µg/ml) of leaf and bark has an effect on the percentage viability (101±4.04) and 97±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99±2.89 and 103±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica. Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl4 exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera

2009 ◽  
Vol 47 (6) ◽  
pp. 1109-1116 ◽  
Author(s):  
Brahma N. Singh ◽  
B.R. Singh ◽  
R.L. Singh ◽  
D. Prakash ◽  
R. Dhakarey ◽  
...  
Keyword(s):  
Dna Damage ◽  
Quorum Sensing ◽  
Moringa Oleifera ◽  
Oxidative Dna Damage ◽  
Protective Activity ◽  
Dna Damage Protective

Acrylamide Exposure and Oxidative DNA Damage, Lipid Peroxidation and Fasting Plasma Glucose Alteration: Association and Mediation Analyses in Chinese Urban Adults

2020 ◽  
Author(s):  
Bin Wang ◽  
Weihong Qiu ◽  
Shijie Yang ◽  
Limin Cao ◽  
Chunmei Zhu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Plasma Glucose ◽  
Fasting Plasma Glucose ◽  
Oxidative Dna Damage ◽  
Adult Population ◽  
Prostaglandin F2α ◽  
Mediation Analyses ◽  
Mediating Role ◽  
Fasting Plasma

<a><b>OBJECTIVE: </b></a>Acrylamide exposure from daily-consumed food has raised global concern.<b> </b>We aimed to assess the exposure-response relationships of internal acrylamide exposure with oxidative DNA damage, lipid peroxidation and fasting plasma glucose (FPG) alteration, and investigate the mediating role of oxidative DNA damage and lipid peroxidation in the association of internal acrylamide exposure with FPG. <p><b>RESEARCH DESIGN AND METHODS:</b> FPG and urinary biomarkers of oxidative DNA damage (8-hydroxy-deoxy-guanosine, 8-OHdG), lipid peroxidation (8-iso-prostaglandin-F2α, 8-iso-PGF2α) and acrylamide exposure (N-acetyl-S-(2-carbamoylethyl)-L-cysteine, AAMA; N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine, GAMA) were measured for 3,270 general adults from the Wuhan-Zhuhai cohort. The associations of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG were assessed by linear mixed models. The mediating roles of 8-OHdG and 8-iso-PGF2α were evaluated by mediation analysis.</p> <p><b>RESULTS:</b> We found significant linear positive dose-response relationships of urinary acrylamide metabolites with 8-OHdG, 8-iso-PGF2α and FPG (except GAMA with FPG), and 8-iso-PGF2α with FPG. Each 1-unit increase in log-transformed level of AAMA, ΣUAAM (AAMA+GAMA) or 8-iso-PGF2α was associated with a 0.17-, 0.15- or 0.23-mmol/L increase in FPG, respectively (<i>P </i>or/and<i> P trend</i><0.05). Each 1% increase in AAMA, GAMA or ΣUAAM was associated with a 0.19%, 0.27% or 0.22% increase in 8-OHdG, respectively, and a 0.40%, 0.48% or 0.44% increase in 8-iso-PGF2α, respectively (<i>P </i>and<i> P trend</i><0.05). Increased 8-iso-PGF2α rather than 8-OHdG significantly mediated 64.29% and 76.92% of the AAMA and ΣUAAM associated-FPG increases, respectively.</p> <p><b>CONCLUSIONS:</b> Exposure of general adult population to acrylamide was associated with FPG elevation, oxidative DNA damage and lipid peroxidation, which in turn partly mediated acrylamide-associated FPG elevation.<b></b></p>

Serum of 8-OHdG as a potential biomarker of oxidative DNA damage in workers exposed to harmful working environments

2020 ◽  
pp. 783-783
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Blood Serum ◽  
Oxidative Dna Damage ◽  
Working Environment ◽  
Working Environments ◽  
Potential Biomarker ◽  
High Level ◽  
Damage Marker

In the blood serum of 70% individuals exposed to harmful factors of the working environment, a high level of oxidative stress and the DNA damage marker 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) were detected.

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