scholarly journals In vitro Antioxidant Analysis and the DNA Damage Protective Activity of Leaf Extract of the Excoecaria agallocha Linn Mangrove Plant

10.5772/55416 ◽  
2013 ◽  
Author(s):  
C. Asha ◽  
M.S. Resmi ◽  
E.V. Soniy
Keyword(s):  
Dna Damage ◽  
Leaf Extract ◽  
Protective Activity ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Dna Damage Protective

Evaluation of the DNA damage protective activity of the germinated peanut (Arachis hypogaea) in relation to antioxidant and anti-inflammatory activity

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 259-268 ◽  
Author(s):  
Apinun Limmongkon ◽  
Jintana Pankam ◽  
Thapakorn Somboon ◽  
Pakwuan Wongshaya ◽  
Phatcharaporn Nopprang
Keyword(s):  
Dna Damage ◽  
Arachis Hypogaea ◽  
Inflammatory Activity ◽  
Protective Activity ◽  
Anti Inflammatory ◽  
Dna Damage Protective

scholarly journals OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

2018 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Andab Pant
Keyword(s):  
Dna Damage ◽  
Hepg2 Cells ◽  
Oxidative Dna Damage ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Protective Activity ◽  
Trypan Blue Exclusion ◽  
Dna Damage Protective

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH•) and hepatoprotective activity against CCl4 induced toxicity in HepG2 liver cells.Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton’s reagent. Protection of human liver-derived HepG2 cells against CCl4 induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 µmol/ml of CCl4 for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 µg/ml.Results: The low dose (0.001-0.1 µg/ml) of leaf and bark has an effect on the percentage viability (101±4.04) and 97±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99±2.89 and 103±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica. Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl4 exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

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