Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption

2009 ◽  
Vol 149 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Helmut Segner
Keyword(s):  
Endocrine Disruption ◽  
Danio Rerio ◽  
Model Organism

scholarly journals Ecotoxicological Effect of Single and Combined Exposure of Carbamazepine and Cadmium on Female Danio rerio: A Multibiomarker Study

2019 ◽  
Vol 9 (7) ◽  
pp. 1362 ◽  
Author(s):  
Chunhong Shi ◽  
Yide He ◽  
Jiaxin Liu ◽  
Yue Lu ◽  
Yuting Fan ◽  
...  
Keyword(s):  
Endocrine Disruption ◽  
Danio Rerio ◽  
Antioxidant Systems ◽  
Aquatic Environments ◽  
Toxicity Profile ◽  
Combined Exposure ◽  
Single Exposure ◽  
Aquatic Vertebrates ◽  
Ecotoxicological Effect ◽  
Environmental Relevance

In aquatic environments, organisms are exposed to mixtures of pollutants which may change the toxicity profile of each contaminant, compared to its toxicity alone. Carbamazepine (CBZ) and cadmium (Cd) are among the pollutants that co-occur in aquatic environments. To date, most research about their toxicity towards aquatic vertebrates is based on single exposure experiments. The present study aims to evaluate single and combined effects of CBZ and Cd on biomarkers in female Danio rerio (zebrafish) by exposing them to environmentally relevant concentrations of these two pollutants for ten days. Four kinds of biomarkers involved in antioxidant systems, energy metabolism, nervous system, and endocrine disruption, respectively, were studied. Our research results coincided with those of former studies in single exposure experiments. However, the combined exposure of CBZ and Cd exerted different responses from other studies in which these two contaminants were examined alone in zebrafish. The present study evidenced the need to conduct more coexposure studies to enhance the environmental relevance of these experimental results.

Chronic exposure of zebrafish (Danio rerio) to flutolanil leads to endocrine disruption and reproductive disorders

2020 ◽  
Vol 184 ◽  
pp. 109310 ◽  
Author(s):  
Miaomiao Teng ◽  
Chen Wang ◽  
Min Song ◽  
Xiangguang Chen ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Endocrine Disruption ◽  
Danio Rerio ◽  
Chronic Exposure ◽  
Reproductive Disorders

Engaging Undergraduates in the Scientific Process: Exploring Invertebrate Endocrine Disruption

2016 ◽  
Vol 78 (5) ◽  
pp. 410-416 ◽  
Author(s):  
Scott Layton ◽  
Jason Belden
Keyword(s):  
Endocrine Disruption ◽  
Endocrine Disrupting Chemicals ◽  
Model Organism ◽  
Female Offspring ◽  
Reproduction Rate ◽  
Interest In Science ◽  
Endocrine Disrupting ◽  
Set Up ◽  
Reproductive Study ◽  
Analyze Data

Engaging students in the process of science to increase learning and critical thinking has become a key emphasis in undergraduate education. Introducing environmental topics, such as the effects of endocrine-disrupting chemicals, into undergraduate courses offers a new means to increase student engagement. Daphnia magna can serve as a model organism for endocrine disruption, and its ease of handling, rapid reproduction rate, and clearly defined endpoints make it useful in short-term, student research projects. The concept of endocrine disruption can be tested through a 21-day reproductive study of D. magna exposed to varying concentrations of the pesticide fenoxycarb. Students will observe an altered reproduction rate and increased production of males under conditions that would typically result only in the production of female offspring. This research system allows students to formulate hypotheses, set up experiments, analyze data, and present results, leading to a greater appreciation of and interest in science.

Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio)

2007 ◽  
Vol 30 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Paul M. Craig ◽  
Chris M. Wood ◽  
Grant B. McClelland
Keyword(s):  
Danio Rerio ◽  
Metal Toxicity ◽  
Model Organism ◽  
Fold Increase ◽  
Soft Water ◽  
Membrane Remodeling ◽  
Protein Levels ◽  
High Degree ◽  
Carbonic Anhydrase 1 ◽  
Mrna Expression Levels

Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na+-K+-ATPase (NKA) α1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na+/H+ exchanger (NHE-2), V-type proton (H+)-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H+-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).

Histopathology as a tool for the evaluation of endocrine disruption in zebrafish (Danio rerio)

2003 ◽  
Vol 22 (4) ◽  
pp. 908-913 ◽  
Author(s):  
Leo T. M. van der Ven ◽  
Piet W. Wester ◽  
Jeff G. Vos
Keyword(s):  
Endocrine Disruption ◽  
Danio Rerio

scholarly journals Effect of β-Cyclodextrin Stabilized Silver Nanoparticles on Gills, Kidney, Liver of Danio rerio

2021 ◽  
Vol 11 (1) ◽  
pp. 2981-2995
Keyword(s):  
Silver Nanoparticles ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Antimicrobial Property ◽  
Research Work ◽  
Model Organism ◽  
Histopathological Study ◽  
Histopathological Studies ◽  
Kidney Liver ◽  
Drug Studies

The silver nanoparticles (AgNP) are applied broadly in medical applications due to their antimicrobial property. However, the toxicity (uptake, translocation, and accumulation) of these AgNPs nanomaterial has not been much explored. Also, cyclodextrin has been used for different pharmaceutical applications due to its various potential properties. Therefore, the toxicity of these AgNPs and cyclodextrin in the model organism such as Danio rerio could be crucial for future nano-drug studies. The main aim of the present research work is to synthesize, characterize biopolymeric silver nanoparticles, and perform histopathological studies of synthesized silver nanoparticles on Danio rerio. The silver nanoparticles of 130nm size have been synthesized successfully using β-Cyclodextrin (β-CD) at room temperature. The various analytical applied to characterized the β-Cyclodextrin (β-CD) reduced silver nanoparticles (β-CD)-SNPs. A histopathological study has been conducted to evaluate the toxicity effect of the synthesized (β-CD)-SNP on the animal model Danio rerio. The (β-CD)-SNPs conc. 30µl/l is affecting and damaged gills and kidney the Danio rerio organs (gills, kidney) exposed after 10 days, yet the liver was found to be healthy. In conclusion, the Danio rerio gills, kidneys, and liver are sensitive to the 130nm-sized (β-CD)-SNPs. The nanoparticles' toxicity depends on concentration; less concentration (30µl/l) accumulates and is absorbed efficiently than the higher concentration (300µl/l). However, no morphological changes were observed on Danio rerio.

scholarly journals Zebrafish (Danio rerio) as a Model Organism

2019 ◽  
Author(s):  
Farmanur Rahman Khan ◽  
Saleh Sulaiman Alhewairini
Keyword(s):  
Danio Rerio ◽  
Model Organism

scholarly journals Detection of biomarkers to differentiate endocrine disruption from hepatotoxicity in zebrafish (Danio rerio) using proteomics

Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124970 ◽  
Author(s):  
Steve U. Ayobahan ◽  
Sebastian Eilebrecht ◽  
Lisa Baumann ◽  
Matthias Teigeler ◽  
Henner Hollert ◽  
...  
Keyword(s):  
Endocrine Disruption ◽  
Danio Rerio ◽  
Detection Of Biomarkers
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