scholarly journals Zebrafish (Danio rerio) as a Model Organism

2019 ◽  
Author(s):  
Farmanur Rahman Khan ◽  
Saleh Sulaiman Alhewairini
Keyword(s):  
Danio Rerio ◽  
Model Organism

Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio)

2007 ◽  
Vol 30 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Paul M. Craig ◽  
Chris M. Wood ◽  
Grant B. McClelland
Keyword(s):  
Danio Rerio ◽  
Metal Toxicity ◽  
Model Organism ◽  
Fold Increase ◽  
Soft Water ◽  
Membrane Remodeling ◽  
Protein Levels ◽  
High Degree ◽  
Carbonic Anhydrase 1 ◽  
Mrna Expression Levels

Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na+-K+-ATPase (NKA) α1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na+/H+ exchanger (NHE-2), V-type proton (H+)-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H+-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).

scholarly journals Effect of β-Cyclodextrin Stabilized Silver Nanoparticles on Gills, Kidney, Liver of Danio rerio

2021 ◽  
Vol 11 (1) ◽  
pp. 2981-2995
Keyword(s):  
Silver Nanoparticles ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Antimicrobial Property ◽  
Research Work ◽  
Model Organism ◽  
Histopathological Study ◽  
Histopathological Studies ◽  
Kidney Liver ◽  
Drug Studies

The silver nanoparticles (AgNP) are applied broadly in medical applications due to their antimicrobial property. However, the toxicity (uptake, translocation, and accumulation) of these AgNPs nanomaterial has not been much explored. Also, cyclodextrin has been used for different pharmaceutical applications due to its various potential properties. Therefore, the toxicity of these AgNPs and cyclodextrin in the model organism such as Danio rerio could be crucial for future nano-drug studies. The main aim of the present research work is to synthesize, characterize biopolymeric silver nanoparticles, and perform histopathological studies of synthesized silver nanoparticles on Danio rerio. The silver nanoparticles of 130nm size have been synthesized successfully using β-Cyclodextrin (β-CD) at room temperature. The various analytical applied to characterized the β-Cyclodextrin (β-CD) reduced silver nanoparticles (β-CD)-SNPs. A histopathological study has been conducted to evaluate the toxicity effect of the synthesized (β-CD)-SNP on the animal model Danio rerio. The (β-CD)-SNPs conc. 30µl/l is affecting and damaged gills and kidney the Danio rerio organs (gills, kidney) exposed after 10 days, yet the liver was found to be healthy. In conclusion, the Danio rerio gills, kidneys, and liver are sensitive to the 130nm-sized (β-CD)-SNPs. The nanoparticles' toxicity depends on concentration; less concentration (30µl/l) accumulates and is absorbed efficiently than the higher concentration (300µl/l). However, no morphological changes were observed on Danio rerio.

scholarly journals The Importance of Zebrafish in Biomedical Research

2013 ◽  
Vol 26 (5) ◽  
pp. 583
Author(s):  
Bárbara Tavares ◽  
Susana Santos Lopes
Keyword(s):  
Biomedical Research ◽  
Drug Screening ◽  
Danio Rerio ◽  
Scientific Literature ◽  
Model Organism ◽  
Toxicity Testing ◽  
Vertebrate Development ◽  
Automated Systems ◽  
Ideal Model ◽  
Research Drug

Introduction: Zebrafish (Danio rerio) is an ideal model organism for the study of vertebrate development. This is due to the large clutches that each couple produces, with up to 200 embryos every 7 days, and to the fact that the embryos and larvae are small, transparent and undergo rapid external development.Material and Methods: Using scientific literature research tools available online and the keywords Zebrafish, biomedical research, human disease, and drug screening, we reviewed original studies and reviews indexed in PubMed.Results: In this review we summarized work conducted with this model for the advancement of our knowledge related to several human diseases. We also focused on the biomedical research being performed in Portugal with the zebrafish model.Discussion: Powerful live imaging and genetic tools are currently available for zebrafish making it a valuable model in biomedicalresearch. The combination of these properties with the optimization of automated systems for drug screening has transformed the zebrafish into a “top model” in biomedical research, drug discovery and toxicity testing. Furthermore, with the optimization of xenografts technology it will be possible to use zebrafish to aide in the choice of the best therapy for each patient.Conclusion: Zebrafish is an excellent model organism in biomedical research, drug development and in clinical therapy.

scholarly journals Assessing the toxicity of Pb- and Sn-based perovskite solar cells in model organism Danio rerio

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Aslihan Babayigit ◽  
Dinh Duy Thanh ◽  
Anitha Ethirajan ◽  
Jean Manca ◽  
Marc Muller ◽  
...  
Keyword(s):  
Solar Cells ◽  
Danio Rerio ◽  
Perovskite Solar Cells ◽  
Model Organism

Zebrafish (Danio rerio) as a Model Organism for Dementia

2010 ◽  
pp. 255-269
Author(s):  
Rob Willemsen ◽  
Sandra van’t Padje ◽  
John C. van Swieten ◽  
Ben A. Oostra
Keyword(s):  
Danio Rerio ◽  
Model Organism

scholarly journals Yolk–Water Partitioning of Neutral Organic Compounds in the Model Organism Danio rerio

2020 ◽  
Vol 39 (8) ◽  
pp. 1506-1516
Author(s):  
Nadin Ulrich ◽  
Nicole Schweiger ◽  
Andrea Pfennigsdorff ◽  
Stefan Scholz ◽  
Kai‐Uwe Goss
Keyword(s):  
Organic Compounds ◽  
Danio Rerio ◽  
Model Organism ◽  
Water Partitioning ◽  
Neutral Organic Compounds

scholarly journals Polyethylene glycol (5,000) succinate conjugate of lopinavir and its associated toxicity using Danio rerio as a model organism

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Oluwole Samuel Aremu ◽  
Lebogang Katata-Seru ◽  
Zimbili Mkhize ◽  
Tarryn Lee Botha ◽  
Victor Wepener
Keyword(s):  
Polyethylene Glycol ◽  
Danio Rerio ◽  
Model Organism

scholarly journals Toxicity of Aqueous L-Selenomethionine and Tert-Butyl Hydroperoxide Exposure to Zebrafish (Danio rerio) Embryos Following Tert-Butyl Hydroquinone Treatment

Toxics ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 44 ◽  
Author(s):  
Allyson K. Gerhart ◽  
David M. Janz
Keyword(s):  
Danio Rerio ◽  
Model Organism ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Range Finding ◽  
Tert Butyl Hydroperoxide ◽  
Waterborne Exposure ◽  
Pre Treatment ◽  
Embryotoxic Effects ◽  
Danio Rerio Embryos

Aqueous L-selenomethionine (SeMet) embryo exposures represent a rapid and simplified method for investigating the embryotoxic effects of SeMet. Using zebrafish (Danio rerio) as a model organism, the objective of the present study was to characterize the effects of waterborne exposure to both SeMet and tert-butyl hydroperoxide (tBOOH) to early life stages of zebrafish pre-treated with the antioxidant tert-butyl hydroquinone (tBHQ) in an attempt to investigate the mechanism of Se toxicity as it relates to oxidative stress. During the initial concentration range finding experiment, recently fertilized embryos were exposed for five days to 5, 25, 125, and 625 µg Se/L (as SeMet). These exposures informed the second experiment in which embryos were exposed to two concentrations of SeMet (25 and 125 µg Se/L) and 75 mg/L tBOOH either with (tBOOH-t, 25-t, 125-t) or without (tBOOH, 25, 125) a 4 h 100 µg/L tBHQ pre-treatment. Survival, hatchability, time to hatch, the frequency and severity of deformities (total and type), and changes in the expression of seven antioxidant-associated genes were determined. Exposures to SeMet and tBOOH reduced hatchability, increased time to hatch, decreased survival, increased the incidence and severity of deformities, and increased glutathione-disulfide reductase (gsr) expression in the pre-treated tBOOH treatment group.

Zebrafish: an animal model for research in veterinary medicine

2015 ◽  
Vol 18 (3) ◽  
pp. 663-674 ◽  
Author(s):  
N. Nowik ◽  
P. Podlasz ◽  
A. Jakimiuk ◽  
N. Kasica ◽  
W. Sienkiewicz ◽  
...  
Keyword(s):  
Animal Model ◽  
Veterinary Medicine ◽  
Danio Rerio ◽  
Bacterial Infections ◽  
Vibrio Anguillarum ◽  
Model Organism ◽  
Time Lapse ◽  
Zebrafish Model ◽  
Real Time Analysis ◽  
Behavioral Studies

Abstract The zebrafish (Danio rerio) has become known as an excellent model organism for studies of vertebrate biology, vertebrate genetics, embryonal development, diseases and drug screening. Nevertheless, there is still lack of detailed reports about usage of the zebrafish as a model in veterinary medicine. Comparing to other vertebrates, they can lay hundreds of eggs at weekly intervals, externally fertilized zebrafish embryos are accessible to observation and manipulation at all stages of their development, which makes possible to simplify the research techniques such as fate mapping, fluorescent tracer time-lapse lineage analysis and single cell transplantation. Although zebrafish are only 2.5 cm long, they are easy to maintain. Intraperitoneal and intracerebroventricular injections, blood sampling and measurement of food intake are possible to be carry out in adult zebrafish. Danio rerio is a useful animal model for neurobiology, developmental biology, drug research, virology, microbiology and genetics. A lot of diseases, for which the zebrafish is a perfect model organism, affect aquatic animals. For a part of them, like those caused by Mycobacterium marinum or Pseudoloma neutrophila, Danio rerio is a natural host, but the zebrafish is also susceptible to the most of fish diseases including Itch, Spring viraemia of carp and Infectious spleen and kidney necrosis. The zebrafish is commonly used in research of bacterial virulence. The zebrafish embryo allows for rapid, non-invasive and real time analysis of bacterial infections in a vertebrate host. Plenty of common pathogens can be examined using zebrafish model: Streptococcus iniae, Vibrio anguillarum or Listeria monocytogenes. The steps are taken to use the zebrafish also in fungal research, especially that dealing with Candida albicans and Cryptococcus neoformans. Although, the zebrafish is used commonly as an animal model to study diseases caused by external agents, it is also useful in studies of metabolic disorders including fatty liver disease and diabetes. The zebrafish is also a valuable tool as a model in behavioral studies connected with feeding, predator evasion, habituation and memory or lateralized control of behavior. The aim of the present article is to familiarize the reader with the possibilities of Danio rerio as an experimental model for veterinary medicine.

scholarly journals Histological Study of the Immune System in Zebrafish, Danio Rerio (Hamilton, 1822)

2021 ◽  
Vol 23 (2) ◽  
pp. 21-28
Author(s):  
Azin Azar ◽  
Zahra Khoshnood
Keyword(s):  
Immune System ◽  
Danio Rerio ◽  
Histological Study ◽  
Abdominal Cavity ◽  
Posterior Part ◽  
Model Organism ◽  
Apical Part ◽  
The Body ◽  
Laboratory Model ◽  
Hematopoietic Tissue

Abstract The aim of the present study was to investigate the cellular characteristics of the immune tissues of Zebrafish, Danio rerio (Hamilton, 1822). The fish were fixed in Bouin՚s solution for 24 hours then dehydrated, cleared, paraffinized, embedded and finally sectioned, stained and observed through optical microscopy. Results showed that immune system tissues of Zebrafish include the apical part of the kidney, thymus, and spleen. The apical part of the kidney was composed of hematopoietic tissue containing blood and immune cells. The spleen was a single organ located at the abdominal cavity containing melanomacrophages. The thymus was observed as a paired organ at the posterior part of the branchial cavity. Results showed that the immune system of the Zebrafish was dispersed in several organs of the body and that this species could be used as a laboratory model organism in immune system studies.

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