Methylation-blocked enzymatic recycling amplification for highly sensitive fluorescence sensing of DNA methyltransferase activity

The Analyst ◽  
2013 ◽  
Vol 138 (1) ◽  
pp. 284-289 ◽  
Author(s):  
Feng Chen ◽  
Yongxi Zhao
Keyword(s):  
Dna Methyltransferase ◽  
Fluorescence Sensing ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Recycling Amplification

Viral cDNA-based extension for highly sensitive fluorescence detection of DNA methyltransferase activity

2018 ◽  
Vol 255 ◽  
pp. 3488-3494 ◽  
Author(s):  
Ying Wang ◽  
Binxiao Li ◽  
Ningbo Zhang ◽  
Dongmei Xi ◽  
Jing Liu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Dna Methyltransferase ◽  
Methyltransferase Activity ◽  
Highly Sensitive ◽  
Viral Cdna

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots

The Analyst ◽  
2021 ◽  
Author(s):  
Yuyang Huang ◽  
Liyun Deng ◽  
Di Su ◽  
Xiangyi Huang ◽  
Jicun Ren
Keyword(s):  
Dna Methyltransferase ◽  
Cell Function ◽  
Vital Role ◽  
Correlation Spectroscopy ◽  
Methyltransferase Activity ◽  
Fluorescence Correlation ◽  
Genetic Imprinting ◽  
Highly Sensitive ◽  
Polymer Dots ◽  
Highly Sensitive Detection

DNA methylation is a critical part of epigenetics and plays a vital role in maintaining normal cell function, genetic imprinting, and human tumorigenesis. Thus, it is important to develop a...

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