In situ nitrate reductase activity as an indicator of nitrate availability

1987 ◽  
Vol 102 (1) ◽  
pp. 145-148 ◽  
Author(s):  
L. Passama ◽  
A. Gojon ◽  
P. Robin ◽  
L. Salsac
1986 ◽  
Vol 16 (6) ◽  
pp. 1165-1169 ◽  
Author(s):  
P. Högberg ◽  
A. Granström ◽  
T. Johansson ◽  
A. Lundmark-Thelin ◽  
T. Näsholm

The nitrate reductase activity of Deschampsiaflexuosa (L.) Trin., an evergreen grass species common in northern coniferous forests, was tested as an indicator of nitrate availability in forest soils. A positive linear correlation (correlation coefficient significantly different from zero, p < 0.01) was found between nitrate supply and nitrate reductase activity in shoots of seedlings in sand cultures. Throughout a vegetation period the nitrate reductase activity of D. flexuosa was constantly 2–16 times higher in a clear-cut area as compared with an adjacent forest. High enzyme activity, however, indicated significant amounts of nitrate also in the forest during spring and autumn. On plots heavily fertilized with urea the nitrate reductase activity was up to three times higher than on control plots, which suggests considerable nitrate formation on the former. The plant nitrate reductase activity method seemed to be an inexpensive, rapid, nondisturbing, semiquantitative indicator of nitrate availability in soils, particularly for time-course studies and in comparisons between experimental plots. Tests of maximal induction of nitrate reductase activity can reveal to what extent factors other than the supply of nitrate are limiting the activity.


1982 ◽  
Vol 60 (12) ◽  
pp. 2665-2670 ◽  
Author(s):  
D. J. Ursino ◽  
D. M. Hunter ◽  
R. D. Laing ◽  
J. L. S. Keighley

Soybean plants (cv. Harosoy 63) inoculated with rhizobia were germinated from seed and beginning on day 7 after planting were subjected to one of four patterns of nutrient fertilization. One group received a nutrient solution containing 5 mM nitrate, a second group received nitrate-free nutrient solution, and two other groups received nitrate-containing solution either from days 7 to 13 or from days 14 to 20. On day 21 rates of leaf photosynthetic CO2 uptake and nitrate reductase activity were measured, as well as the capacities of the leaf to export recent photosynthate and of the nodules to reduce acetylene. The data support the hypothesis that sufficient nitrate availability in the leaves of young soybean plants can modify both photosynthetic CO2 uptake and light-mediated photoassimilate export to an extent that nodule development and the capacity for nitrogen fixation are reduced.


1981 ◽  
Vol 88 (2) ◽  
pp. 289-297 ◽  
Author(s):  
M. UDAYAKUMAR ◽  
R. DEVENDRA ◽  
V. SREENIVASA REDDY ◽  
K. S. KRISHNA SASTRY

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