scholarly journals Differential In Vitro Flowering Response of Mulberry Shoot Tips As Influenced by Maturity of Explants and Media Composition

2002 ◽  
Author(s):  
Deepu Mathew
Keyword(s):  
Shoot Tips ◽  
In Vitro Flowering ◽  
Media Composition ◽  
Flowering Response

scholarly journals Vegetative propagation of Nidularium fulgens Lem. in vitro.

1984 ◽  
Vol 32 (2) ◽  
pp. 101-106
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans
Keyword(s):  
Vegetative Propagation ◽  
Shoot Tips ◽  
Media Composition ◽  
Liquid Media ◽  
Axillary Shoots ◽  
Solid Agar ◽  
Half Strength ◽  
Agar Media

Shoot tips were isolated and placed on solid agar media (half strength Murashige and Skoog macroelements, 3% sucrose, vitamins, 1 mg l-1 BA and 0.1 mg l-1 NAA). After one month, shoots which had begun to grow were transferred to liquid media (composition as above but with no agar and BA reduced to 0.3 mg l-1). After 12 weeks about 5 axillary shoots per explant were produced. Subsequent planting of these shoots in soil achieved almost 100% rooting. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Encapsulation-dehydration based cryopreservation of Rauwolfia shoot tips and their regeneration potential in vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 82-87
Author(s):  
P. KISKU ◽  
S. SAHU ◽  
U. SALMA ◽  
S. SINHA RAY ◽  
P. SAHA ◽  
...  
Keyword(s):  
Shoot Tips ◽  
Regeneration Potential

scholarly journals Gold nanoparticles affect the cryopreservation efficiency of in vitro-derived shoot tips of bleeding heart

2021 ◽  
Author(s):  
Dariusz Kulus ◽  
Alicja Tymoszuk
Keyword(s):  
Gold Nanoparticles ◽  
Alginate Bead ◽  
Stability Index ◽  
Shoot Tips ◽  
Start Codon ◽  
Lamprocapnos Spectabilis ◽  
Colloid Dispersion ◽  
Recovery Medium ◽  
Alginate Matrix

AbstractThe popularity of nanoparticles (NPs) is continuously increasing. To date, however, there has been little research on the application of NPs in plant cryopreservation, i.e. storage of tissues in liquid nitrogen (LN). The aim of this study is to analyze the effect and evaluate the usefulness of gold nanoparticles (AuNPs) in regard to cryobiology studies. In vitro-derived shoot tips of Lamprocapnos spectabilis ‘Valentine’ were cryopreserved with the encapsulation-vitrification protocol. Gold nanoparticles (at 10–30 ppm concentration; 13 nm in size) were added either into the preculture medium; to the protective bead matrix during encapsulation; or to the recovery medium after rewarming of samples. The control plants were produced from cryopreserved explants non-treated with nanoparticles or treated with colloid dispersion medium without NPs. A non-LN-treated standard was also considered. The influence of AuNPs on the cryopreservation efficiency was determined by evaluating the recovery rate of explants and their morphogenic response; the membrane stability index (MSI); the concentration of pigments in shoots; and the antioxidant enzymes activity. The genetic stability of the plant material was evaluated using Start Codon Targeted Polymorphism (SCoT) markers. It was found that 10 ppm of AuNPs added into the alginate bead matrix improved the recovery level of LN-derived shoot tips (70.0%) compared to the non-NPs-treated cryopreserved control (50.5%). On the other hand, the presence of nanoparticles in the recovery medium had a deleterious effect on the survival of explants. AuNPs usually had no impact on the MSI (73.9–85.9%), except for those added into the recovery medium at the concentration of 30 ppm (decline to 55.8%). All LN-derived shoots were shorter and contained less chlorophyll and carotenoids than the untreated standard. Moreover, the application of AuNPs affected the enzymatic activity in L. spectabilis. Minor genetic variation was found in 8.6% of plants if AuNPs were added either into the preculture medium (at 10 and 20 ppm) or to the alginate matrix (at 30 ppm). In conclusion, AuNPs added at a lower concentration (10 ppm) into the protective bead matrix can significantly improve the cryopreservation efficiency in L. spectabilis with no alternation in the DNA sequence.

In vitro flowering and fruiting in Portulaca pilosa L.

2021 ◽  
Vol 140 ◽  
pp. 1-3
Author(s):  
Yuping Xiong ◽  
Shuangyan Chen ◽  
Zhenpeng Wei ◽  
Xincheng Yu ◽  
Jinhui Pang ◽  
...  
Keyword(s):  
In Vitro Flowering

scholarly journals Rejuvenation of chicory and lettuce plants following phase change in tissue culture

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anthony J. Conner ◽  
Helen Searle ◽  
Jeanne M. E. Jacobs
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Seed Set ◽  
Genetic Manipulation ◽  
Adventitious Shoot ◽  
In Vitro Flowering ◽  
Phase Changes ◽  
Adventitious Shoot Regeneration ◽  
Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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