Isolation and characterization of heterotrophic, aerobic bacteria from oil storage caverns in northern Germany

1994 ◽  
Vol 42 (2-3) ◽  
pp. 463-468
Author(s):  
M. Bock ◽  
P. K�mpfer ◽  
K. Bosecker ◽  
W. Dott
Keyword(s):  
Aerobic Bacteria ◽  
Northern Germany ◽  
Oil Storage ◽  
Isolation And Characterization

Isolation and characterization of highly thermophilic xylanolyticThermus thermophilusstrains from hot composts

2000 ◽  
Vol 46 (11) ◽  
pp. 1029-1035 ◽  
Author(s):  
Pierre-François Lyon ◽  
Trello Beffa ◽  
Michel Blanc ◽  
Georg Auling ◽  
Michel Aragno
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Xylanase Activity ◽  
Thermostable Enzyme ◽  
Aerobic Bacteria ◽  
Maximal Growth ◽  
Detailed Report ◽  
Xylanolytic Activity ◽  
Isolation And Characterization

This is the first detailed report of xylanolytic activity in Thermus strains. Two highly thermophilic xylanolytic bacteria, very closely related to non-xylanolytic T. thermophilus strains, have been isolated from the hottest zones of compost piles. Strain X6 was investigated in more detail. The growth rate (optical density monitoring) on xylan was 0.404·h-1at 75°C. Maximal growth temperature was 81°C. Xylanase activity was mainly cell-bound, but was solubilized into the medium by sonication. It was induced by xylan or xylose in the culture medium. The temperature and pH optima of the xylanases were determined to be around 100°C and pH 6, respectively. Xylanase activity was fairly thermostable; only 39% of activity was lost after an incubation period of 48 h at 90°C in the absence of substrate. Xylanolytic T. thermophilus strains could contribute to the degradation of hemicellulose during the thermogenic phase of industrial composting.Key words: Thermus, thermophilic aerobic bacteria, xylanase, thermostable enzyme, compost.

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

2012 ◽  
Author(s):  
Rosli Md. Illias ◽  
Seok Wei Ooi ◽  
Ahmad Kamal Idris ◽  
Wan Aizan Wan Abdul Rahman
Keyword(s):  
Oil Recovery ◽  
Sole Carbon Source ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Oil Reservoir ◽  
Genus Bacillus ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Naci Concentration

Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

scholarly journals Isolation and Characterization of An Endosulfan-Degrading Strain, Stenotrophomonas sp. LD-6, and its Potential in Soil Bioremediation

2012 ◽  
Vol 61 (4) ◽  
pp. 257-262 ◽  
Author(s):  
FANG-BO YU ◽  
WASEEM ALI SHINAWAR ◽  
ING-YA SUN ◽  
LIN-PING LUO
Keyword(s):  
Sole Source ◽  
Aerobic Bacteria ◽  
Future Research ◽  
Soil Bioremediation ◽  
Isolation And Characterization ◽  
Oxidative Pathway ◽  
Degradative Enzymes ◽  
Endosulfan Diol ◽  
Cell Crude Extract

Aerobic bacteria degrading endosulfan were isolated from contaminated sludge. One of the isolates, LD-6, was identified as Stenotrophomonas sp. The bacterium could utilize endosulfan as the sole source of carbon and sulfur. 100 mg/l endosulfan was completely degraded within 10 days, and endosulfan diol and endosulfan ether were detected as major metabolites with a slight decrease in culture pH. The results indicated that Stenotrophomonas. sp. LD-6 might degrade endosulfan by a non-oxidative pathway. Biodegradation of both isomers was relatively better at a temperature range of 25-35 degrees C, with a maximum at 30 degrees C. In addition, cell crude extract of strain LD-6 could metabolize endosulfan rapidly, and degradative enzymes were intracellular distributed and constitutively expressed. Besides, application of the strain was found to promote the removal of endosulfan in soil. This study might help with the future research in better understanding of the biodegradation.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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