Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

2012 ◽  
Author(s):  
Rosli Md. Illias ◽  
Seok Wei Ooi ◽  
Ahmad Kamal Idris ◽  
Wan Aizan Wan Abdul Rahman
Keyword(s):  
Oil Recovery ◽  
Sole Carbon Source ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Oil Reservoir ◽  
Genus Bacillus ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Naci Concentration

Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

scholarly journals Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal
Keyword(s):  
Ph Value ◽  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Nano Materials ◽  
Elongation At Break ◽  
Stable Suspension ◽  
Average Size ◽  
Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir

Extremophiles ◽  
2001 ◽  
Vol 5 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Margarita Miroshnichenko ◽  
Hans Hippe ◽  
Erko Stackebrandt ◽  
Nadezhda Kostrikina ◽  
Nikolai Chernyh ◽  
...  
Keyword(s):  
High Temperature ◽  
Western Siberia ◽  
Oil Reservoir ◽  
Isolation And Characterization

scholarly journals Characterization of Complex Crude Oil Microemulsions-DSC Contribution

2018 ◽  
Vol 73 ◽  
pp. 3 ◽  
Author(s):  
Ayako Fukumoto ◽  
Christine Dalmazzone ◽  
Didier Frot ◽  
Loïc Barré ◽  
Christine Noïk
Keyword(s):  
Crude Oil ◽  
Oil Recovery ◽  
Continuous Phase ◽  
Morphological Characterization ◽  
Model System ◽  
Residual Oil ◽  
Surfactant Flooding ◽  
Interfacial Tensions ◽  
Physico Chemical

Surfactant flooding is a chemical enhanced oil recovery (EOR) process which consists in injecting optimized formulations of surfactants in the reservoir in order to remobilize the residual oil trapped in the pores of the rock. To do that, it is necessary to design specific formulations in order to get so-called Winsor III systems of very low interfacial tensions with the crude oil. Unfortunately, there is no well-established way to characterize and understand the physical properties and structures of microemulsions composed of crude oil and industrial surfactants due to their extreme complexity. In a previous work, we have developed a methodology based on the use of several techniques (DLS, MLS, SAXS, cryo-SEM, DSC, interfacial measurements, etc.) allowing physico-chemical and morphological characterization of these microemulsions in the case of a model system. In this article, we will demonstrate how DSC can be used to provide information on the physico-chemical composition of complex microemulsions (water and oil content, salinity, etc.) and on their morphology (continuous phase, dispersed phase, etc.).

scholarly journals Isolation and Characterization of Biosurfactant-Producing Bacteria from Amapaense Amazon Soils

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Elisa Maria de Oliveira ◽  
Victor Hugo Gomes Sales ◽  
Marcelo Silva Andrade ◽  
Jerri Édson Zilli ◽  
Wardsson Lustrino Borges ◽  
...  
Keyword(s):  
Surface Tension ◽  
Gene Sequencing ◽  
Biotechnological Applications ◽  
16S Rdna Gene ◽  
Upland Forest ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Emulsification Capacity ◽  
16S Rdna Gene Sequencing

The objective of this research was to perform screening of biosurfactant-producing bacteria from Amapaense Amazon soils. Floodplain- and upland-forest soils of three municipalities of the Amapá state were isolated and identified. The isolates were cultured in nutrient broth with olive oil, and their extracts were evaluated according to drop collapse, oil dispersion, emulsification, and surface tension tests. From three hundred and eighteen isolates, the 43 bacteria were selected and identified by 16S rDNA gene sequencing, indicating the presence of three different genera, Serratia, Paenibacillus, and Citrobacter. The extracellular biosurfactant production pointed out the 15 most efficient bacteria that presented high emulsification capacity (E24 > 48%) and stability (less than 10% of drop after 72 h) and great potential to reduce the surface tension (varying from 49.40 to 34.50 mN·m−1). Cluster analysis classified genetically related isolates in different groups, which can be connected to differences in the amount or the sort of biosurfactants. Isolates from Serratia genus presented better emulsification capacity and produced a more significant surface tension drop, indicating a promising potential for biotechnological applications.

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