Isolation and Characterization of Gram-Positive Cyanophycin-Degrading BacteriaKinetic Studies on Cyanophycin Depolymerase Activity in Aerobic Bacteria

2004 ◽  
Vol 5 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Martin Obst ◽  
Ahmed Sallam ◽  
Heinrich Luftmann ◽  
Alexander Steinbüchel
Keyword(s):  
Aerobic Bacteria ◽  
Gram Positive ◽  
Isolation And Characterization

Isolation and characterization of the peptidoglycans from selected Gram-positive and Gram-negative periodontal pathogens

1985 ◽  
Vol 31 (2) ◽  
pp. 154-160 ◽  
Author(s):  
M. R. Barnard ◽  
S. C. Holt
Keyword(s):  
Electron Microscopy ◽  
Diaminopimelic Acid ◽  
Muramic Acid ◽  
Periodontal Pathogens ◽  
Gram Positive ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Bacteroides Gingivalis ◽  
Scanning Electron

The peptidoglycans from several Gram-negative and Gram-positive periodontal pathogens were isolated, purified, and characterized both morphologically and chemically. In addition, the effects of the mureolytic enzymes, lysozyme, M-1 N-acetyl-muramidase, and the AM-3 endopeptidase, on the peptidoglycans were examined. These enzymes were found to be highly effective in the degradation of the purified peptidoglycans; however, a Bacteroides capillus peptidoglycan–protein complex exhibited a greater resistance to these enzymes. Morphologically, the peptidoglycans consisted of large saccular sheets which, when viewed by scanning electron microscopy, contained numerous holes and tears. Chemically, the peptidoglycans consisted of muramic acid, glucosamine, alanine, glutamic acid, and meso-diaminopimelic acid (DAP). One Bacteroides species, Bacteroides gingivalis strain W, contained glycine and LL-DAP, suggestive of an indirectly cross-linked A3γ peptidoglycan.

scholarly journals Isolation and Characterization of Indigenous Lactic Acid Bacteria from Sago Wastewater

2018 ◽  
Author(s):  
Pauline Destinugrainy Kasi ◽  
Ariandi ◽  
Heni Mutmainnah
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Agar Medium ◽  
Gram Positive ◽  
Processing Industry ◽  
Isolation And Purification ◽  
South Sulawesi ◽  
Isolation And Characterization

Sago extraction processing into sago flour is generally still done in a traditional or semi-mechanical way, with less attention to aspects of hygiene, and resulted sago fermentation spontaneously. This study aimed to isolate and characterize indigenous lactic acid bacteria (LAB) from sago wastewater which obtained directly from the traditional sago processing industry in Malangke Barat, South Sulawesi. The sago wastewater was stored for 1, 3 and 7 days of fermentation. The research was conducted on the LAB isolation and purification on MRS agar medium supplemented with 0.5% CaCO3. Two purified putative LAB isolate were each obtained from 1 day fermentation and 3 days fermentation. The LAB isolate from 1 day fermentation has milky white colors and smooth-round colonies, while LAB isolates from 3 days fermentation has creamy colors and smooth-round colonies. Both of the isolate were gram positive-rods, non-motile and negative catalase reaction.

Isolation and characterization of highly thermophilic xylanolyticThermus thermophilusstrains from hot composts

2000 ◽  
Vol 46 (11) ◽  
pp. 1029-1035 ◽  
Author(s):  
Pierre-François Lyon ◽  
Trello Beffa ◽  
Michel Blanc ◽  
Georg Auling ◽  
Michel Aragno
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Xylanase Activity ◽  
Thermostable Enzyme ◽  
Aerobic Bacteria ◽  
Maximal Growth ◽  
Detailed Report ◽  
Xylanolytic Activity ◽  
Isolation And Characterization

This is the first detailed report of xylanolytic activity in Thermus strains. Two highly thermophilic xylanolytic bacteria, very closely related to non-xylanolytic T. thermophilus strains, have been isolated from the hottest zones of compost piles. Strain X6 was investigated in more detail. The growth rate (optical density monitoring) on xylan was 0.404·h-1at 75°C. Maximal growth temperature was 81°C. Xylanase activity was mainly cell-bound, but was solubilized into the medium by sonication. It was induced by xylan or xylose in the culture medium. The temperature and pH optima of the xylanases were determined to be around 100°C and pH 6, respectively. Xylanase activity was fairly thermostable; only 39% of activity was lost after an incubation period of 48 h at 90°C in the absence of substrate. Xylanolytic T. thermophilus strains could contribute to the degradation of hemicellulose during the thermogenic phase of industrial composting.Key words: Thermus, thermophilic aerobic bacteria, xylanase, thermostable enzyme, compost.

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

2012 ◽  
Author(s):  
Rosli Md. Illias ◽  
Seok Wei Ooi ◽  
Ahmad Kamal Idris ◽  
Wan Aizan Wan Abdul Rahman
Keyword(s):  
Oil Recovery ◽  
Sole Carbon Source ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Oil Reservoir ◽  
Genus Bacillus ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Naci Concentration

Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

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