A new method of quantitative detection of Chalara elegans and C. thielavioides in soils using carrot discs

2012 ◽  
Vol 119 (5-6) ◽  
pp. 169-173
Author(s):  
Werner E Heller
The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3909-3915 ◽  
Author(s):  
Xu Wu ◽  
Liping Wang ◽  
Yan Peng ◽  
Fang Wu ◽  
Jiumei Cao ◽  
...  

A new method for the qualitative and quantitative detection of direct oral anticoagulant rivaroxaban by terahertz spectroscopy.


2019 ◽  
Vol 216 (12) ◽  
pp. 1800849
Author(s):  
Jonathan Bier ◽  
Michael Keusgen ◽  
Jochen Frey

1983 ◽  
Vol 11 (3) ◽  
pp. 531-534
Author(s):  
Michihiko HAYAKAWA ◽  
Satoru MIURA ◽  
Hidetoshi ENDO ◽  
Yoshio IWATA ◽  
Michitaka NAITO ◽  
...  

TECHNOLOGY ◽  
2013 ◽  
Vol 01 (01) ◽  
pp. 88-96 ◽  
Author(s):  
Tania Konry ◽  
Adam Lerner ◽  
Martin L. Yarmush ◽  
Irina V. Smolina

In this report, we present a new method for sensitive detection of short DNA sites in single cells with single base resolution. The method combines peptide nucleic acid (PNA) openers as the tagging probes, together with isothermal rolling circle amplification (RCA) and fluorescence-based detection, all performed in a cells-in-flow format. Bis-PNAs provide single base resolution, while RCA ensures linear signal amplification. We applied this method to detect the oncoviral DNA inserts in cancer cell lines using a flow-cytometry system. We also demonstrated quantitative detection of the selected signature sites within single cells in microfluidic nano-liter droplets. Our results show single-nucleotide polymorphism (SNP) discrimination and detection of copy-number variations (CNV) under isothermal non-denaturing conditions. This new method is ideal for many applications in which ultra-sensitive DNA characterization with single base resolution is desired on the level of single cells.


Catalysts ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 168 ◽  
Author(s):  
Ye Yuan ◽  
Jia Xu ◽  
Zhenyu Zhao ◽  
Hui Li ◽  
Kai Wang ◽  
...  

In this study, we reported on a novel mimetic peroxidase, deuterohemin–Ala–His–Glu, (Dh–A–H–E). The kinetic parameters of Dh–A–H–E suggested that it was a mimetic peroxidase and followed the ping–pong mechanism. Compared to horseradish peroxidase, Dh–A–H–E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh–A–H–E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.


2017 ◽  
Vol 9 (22) ◽  
pp. 3375-3379 ◽  
Author(s):  
Lang Zhang ◽  
Jinfang Nie ◽  
Huili Wang ◽  
Juanhua Yang ◽  
Bingyue Wang ◽  
...  

A new method is proposed for the quantitative detection of alkaline phosphatase (ALP) by integrating paper microfluidics with an instrument-free length-measuring readout based on the ALP-caused hydrophilicity-to-hydrophobicity change in paper.


Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.


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