Introduction of a New Method for a Quantitative Detection of Biological Analyte

2019 ◽  
Vol 216 (12) ◽  
pp. 1800849
Author(s):  
Jonathan Bier ◽  
Michael Keusgen ◽  
Jochen Frey
Keyword(s):  
New Method ◽  
Quantitative Detection

Quantitative analysis of direct oral anticoagulant rivaroxaban by terahertz spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3909-3915 ◽  
Author(s):  
Xu Wu ◽  
Liping Wang ◽  
Yan Peng ◽  
Fang Wu ◽  
Jiumei Cao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Oral Anticoagulant ◽  
Terahertz Spectroscopy ◽  
New Method ◽  
Quantitative Detection ◽  
Direct Oral Anticoagulant ◽  
Qualitative And Quantitative

A new method for the qualitative and quantitative detection of direct oral anticoagulant rivaroxaban by terahertz spectroscopy.

scholarly journals A New Method for the Quantitative Detection of Platelet Aggregates

1983 ◽  
Vol 11 (3) ◽  
pp. 531-534
Author(s):  
Michihiko HAYAKAWA ◽  
Satoru MIURA ◽  
Hidetoshi ENDO ◽  
Yoshio IWATA ◽  
Michitaka NAITO ◽  
...  
Keyword(s):  
New Method ◽  
Quantitative Detection ◽  
Platelet Aggregates

scholarly journals Target DNA detection and quantitation on a single cell with single base resolution

TECHNOLOGY ◽  
2013 ◽  
Vol 01 (01) ◽  
pp. 88-96 ◽  
Author(s):  
Tania Konry ◽  
Adam Lerner ◽  
Martin L. Yarmush ◽  
Irina V. Smolina
Keyword(s):  
Single Cells ◽  
Rolling Circle Amplification ◽  
Copy Number Variations ◽  
New Method ◽  
Quantitative Detection ◽  
Single Nucleotide ◽  
Rolling Circle ◽  
Single Base ◽  
Target Dna ◽  
Single Base Resolution

In this report, we present a new method for sensitive detection of short DNA sites in single cells with single base resolution. The method combines peptide nucleic acid (PNA) openers as the tagging probes, together with isothermal rolling circle amplification (RCA) and fluorescence-based detection, all performed in a cells-in-flow format. Bis-PNAs provide single base resolution, while RCA ensures linear signal amplification. We applied this method to detect the oncoviral DNA inserts in cancer cell lines using a flow-cytometry system. We also demonstrated quantitative detection of the selected signature sites within single cells in microfluidic nano-liter droplets. Our results show single-nucleotide polymorphism (SNP) discrimination and detection of copy-number variations (CNV) under isothermal non-denaturing conditions. This new method is ideal for many applications in which ultra-sensitive DNA characterization with single base resolution is desired on the level of single cells.

scholarly journals Design and Characterization of a Novel Artificial Peroxidase

Catalysts ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 168 ◽  
Author(s):  
Ye Yuan ◽  
Jia Xu ◽  
Zhenyu Zhao ◽  
Hui Li ◽  
Kai Wang ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Kinetic Parameters ◽  
Organic Solvents ◽  
New Method ◽  
Quantitative Detection ◽  
Ping Pong ◽  
Excellent Stability

In this study, we reported on a novel mimetic peroxidase, deuterohemin–Ala–His–Glu, (Dh–A–H–E). The kinetic parameters of Dh–A–H–E suggested that it was a mimetic peroxidase and followed the ping–pong mechanism. Compared to horseradish peroxidase, Dh–A–H–E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh–A–H–E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.

Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

2017 ◽  
Vol 9 (22) ◽  
pp. 3375-3379 ◽  
Author(s):  
Lang Zhang ◽  
Jinfang Nie ◽  
Huili Wang ◽  
Juanhua Yang ◽  
Bingyue Wang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
New Method ◽  
Quantitative Detection ◽  
Paper Microfluidics ◽  
Free Length

A new method is proposed for the quantitative detection of alkaline phosphatase (ALP) by integrating paper microfluidics with an instrument-free length-measuring readout based on the ALP-caused hydrophilicity-to-hydrophobicity change in paper.

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

Sign in / Sign up

Export Citation Format

Share Document