scholarly journals Design and Characterization of a Novel Artificial Peroxidase

Catalysts ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 168 ◽  
Author(s):  
Ye Yuan ◽  
Jia Xu ◽  
Zhenyu Zhao ◽  
Hui Li ◽  
Kai Wang ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Kinetic Parameters ◽  
Organic Solvents ◽  
New Method ◽  
Quantitative Detection ◽  
Ping Pong ◽  
Excellent Stability

In this study, we reported on a novel mimetic peroxidase, deuterohemin–Ala–His–Glu, (Dh–A–H–E). The kinetic parameters of Dh–A–H–E suggested that it was a mimetic peroxidase and followed the ping–pong mechanism. Compared to horseradish peroxidase, Dh–A–H–E exhibited excellent stability when tested at different pH and temperatures, and using different organic solvents. Based on our above results, a new method using Dh–A–H–E has successfully been developed for the fast and quantitative detection of trace amounts of glucose.

Concanavalin A-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.

1976 ◽  
Vol 24 (8) ◽  
pp. 908-914 ◽  
Author(s):  
R C Allen ◽  
S S Spicer ◽  
D Zehr
Keyword(s):  
Horseradish Peroxidase ◽  
Isoelectric Focusing ◽  
Concanavalin A ◽  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Polyacrylamide Gels ◽  
Periodic Acid Schiff ◽  
Coomassie Blue

The Coomassie Blue protein stain and the periodic acid-Schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. The method utilizes a Concanavalin A-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal N-acetylglucosamine. The method applied to characterization of M and Z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs have revealed differences in carbohydrate content of various components that were previously undetected.

A new method for approximation of closed convex subsets of B(H)

Filomat ◽  
2017 ◽  
Vol 31 (19) ◽  
pp. 6005-6013
Author(s):  
Mahdi Iranmanesh ◽  
Fatemeh Soleimany
Keyword(s):  
Best Approximation ◽  
Numerical Range ◽  
New Method ◽  
C Algebra ◽  
Convex Subsets

In this paper we use the concept of numerical range to characterize best approximation points in closed convex subsets of B(H): Finally by using this method we give also a useful characterization of best approximation in closed convex subsets of a C*-algebra A.

On a generalization of the Rényi–Srivastava characterization of the Poisson law

2021 ◽  
Vol 58 (1) ◽  
pp. 68-82
Author(s):  
Jean-Renaud Pycke
Keyword(s):  
Life Insurance ◽  
Collective Model ◽  
New Method ◽  
Bell Polynomials ◽  
Explicit Formulas ◽  
Compound Distributions ◽  
Poisson Law ◽  
Pierre Loti

AbstractWe give a new method of proof for a result of D. Pierre-Loti-Viaud and P. Boulongne which can be seen as a generalization of a characterization of Poisson law due to Rényi and Srivastava. We also provide explicit formulas, in terms of Bell polynomials, for the moments of the compound distributions occurring in the extended collective model in non-life insurance.

scholarly journals Purification and characterization of fat body lipase from the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae)

2019 ◽  
Vol 81 (1) ◽  
Author(s):  
Rahma R. Z. Mahdy ◽  
Shaimaa A. Mo’men ◽  
Marah M. Abd El-Bar ◽  
Emad M. S. Barakat
Keyword(s):  
Metal Ions ◽  
Kinetic Parameters ◽  
Biochemical Characterization ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Specific Activity ◽  
Larval Instar ◽  
Gel Filtration ◽  
Molecular Weights

Abstract Background Insect lipid mobilization and transport are currently under research, especially lipases and lipophorin because of their roles in the production of energy and lipid transport at a flying activity. The present study has been conducted to purify intracellular fat body lipase for the first time, from the last larval instar of Galleria mellonella. Results Purification methods by combination of ammonium sulfate [(NH4)2SO4] precipitation and gel filtration using Sephadex G-100 demonstrated that the amount of protein and the specific activity of fat body lipase were 0.008633 ± 0.000551 mg/ml and 1.5754 ± 0.1042 μmol/min/mg protein, respectively, with a 98.9 fold purity and recovery of 50.81%. Hence, the sephadex G-100 step was more effective in the purification process. SDS-PAGE and zymogram revealed that fat body lipase showed two monomers with molecular weights of 178.8 and 62.6 kDa. Furthermore, biochemical characterization of fat body lipase was carried out through testing its activities against several factors, such as different temperatures, pH ranges, metal ions, and inhibitors ending by determination of their kinetic parameters with the use of p-nitrophenyl butyrate (PNPB) as a substrate. The highest activities of enzyme were determined at the temperature ranges of 35–37 °C and 37–40 °C and pH ranges of 7–9 and 7–10. The partially purified enzyme showed significant stimulation by Ca2+, K+, and Na+ metal ions indicating that fat body lipase is metalloproteinase. Lipase activity was strongly inhibited by some inhibitors; phenylmethylsulfonyl fluoride (PMSF), ethylene-diaminetetractic acid (EDTA), and ethylene glycoltetraacetic acid (EGTA) providing evidence of the presence of serine residue and activation of enzymes by metal ions. Kinetic parameters were 0.316 Umg− 1 Vmax and 301.95 mM Km. Conclusion Considering the purification of fat body lipase from larvae and the usage of some inhibitors especially ion chelating agents, it is suggested to develop a successful control of Galleria mellonella in near future by using lipase inhibitors.

scholarly journals Spectroscopic characterization of a thermodynamically stable doubly charged diatomic molecule: MgAr2+

2021 ◽  
Author(s):  
Dominik Wehrli ◽  
Matthieu Génévriez ◽  
Frédéric Merkt
Keyword(s):  
High Resolution ◽  
Diatomic Molecule ◽  
Spectroscopic Characterization ◽  
New Method ◽  
High Resolution Spectroscopy ◽  
Singly Charged ◽  
Doubly Charged

We present a new method to study doubly charged molecules relying on high-resolution spectroscopy of the singly charged parent cation, and report on the first spectroscopic characterization of a thermodynamically stable diatomic dication, MgAr2+.

Quantitative analysis of direct oral anticoagulant rivaroxaban by terahertz spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3909-3915 ◽  
Author(s):  
Xu Wu ◽  
Liping Wang ◽  
Yan Peng ◽  
Fang Wu ◽  
Jiumei Cao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Oral Anticoagulant ◽  
Terahertz Spectroscopy ◽  
New Method ◽  
Quantitative Detection ◽  
Direct Oral Anticoagulant ◽  
Qualitative And Quantitative

A new method for the qualitative and quantitative detection of direct oral anticoagulant rivaroxaban by terahertz spectroscopy.

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