Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

2017 ◽  
Vol 9 (22) ◽  
pp. 3375-3379 ◽  
Author(s):  
Lang Zhang ◽  
Jinfang Nie ◽  
Huili Wang ◽  
Juanhua Yang ◽  
Bingyue Wang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
New Method ◽  
Quantitative Detection ◽  
Paper Microfluidics ◽  
Free Length

A new method is proposed for the quantitative detection of alkaline phosphatase (ALP) by integrating paper microfluidics with an instrument-free length-measuring readout based on the ALP-caused hydrophilicity-to-hydrophobicity change in paper.

Quantitative analysis of direct oral anticoagulant rivaroxaban by terahertz spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3909-3915 ◽  
Author(s):  
Xu Wu ◽  
Liping Wang ◽  
Yan Peng ◽  
Fang Wu ◽  
Jiumei Cao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Oral Anticoagulant ◽  
Terahertz Spectroscopy ◽  
New Method ◽  
Quantitative Detection ◽  
Direct Oral Anticoagulant ◽  
Qualitative And Quantitative

A new method for the qualitative and quantitative detection of direct oral anticoagulant rivaroxaban by terahertz spectroscopy.

scholarly journals Alkaline Phosphatase —A New Method of Screening

1960 ◽  
Vol 8 (2) ◽  
pp. 14-16
Author(s):  
A. E. Bertone ◽  
H. A. Sinclaire
Keyword(s):  
Alkaline Phosphatase ◽  
New Method

Introduction of a New Method for a Quantitative Detection of Biological Analyte

2019 ◽  
Vol 216 (12) ◽  
pp. 1800849
Author(s):  
Jonathan Bier ◽  
Michael Keusgen ◽  
Jochen Frey
Keyword(s):  
New Method ◽  
Quantitative Detection

Some Methodological Modifications of the Technicon "SMA 12/60 AutoAnalyzer" System

1972 ◽  
Vol 18 (7) ◽  
pp. 630-642 ◽  
Author(s):  
Tevfik K Bigat ◽  
Abraham Saifer
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Aspartate Aminotransferase ◽  
Inorganic Phosphate ◽  
New Method ◽  
Green Method ◽  
Bromcresol Green ◽  
Made In

Abstract Methodological modifications have been made in our "SMA 12/60 AutoAnalyzer" system, to improve the reproducibility and accuracy of six commonly performed procedures. These include glucose with the glucose oxidase method, albumin with the bromcresol green method, and alkaline phosphatase with the sodium thymolphthalein monophosphate method. We have also modified the composition of the reagents used in the determination of protein, inorganic phosphate, and aspartate aminotransferase. A general approach that is useful for adapting a new method to a multichannel AutoAnalyzer system is discussed.

Collaborative Study of a New Alkaline Phosphatase Assay System for Milk

1968 ◽  
Vol 51 (4) ◽  
pp. 802-807 ◽  
Author(s):  
D H Kleyn ◽  
S H C Lin
Keyword(s):  
Alkaline Phosphatase ◽  
Statistical Study ◽  
Collaborative Study ◽  
Skim Milk ◽  
New Method ◽  
Chi Square ◽  
Alkaline Phosphatase Assay ◽  
Whole Milk ◽  
Phosphatase Assay ◽  
Tests Of Independence

Abstract A new method in which phenolphthalein monophosphate is used as substrate has been developed for determining residual alkaline phosphatase: Upon hydrolysis, phenolphthalein is released and yields a red solution that is compared visually with a standard prepared from the same milk. Seven collaborators made 168 observations that were used in a statistical study of the results. Chi-square calculations and tests of independence indicated that the described method yields results on skim milk, whole milk, and light cream that are as precise and either as accurate or more accurate than those obtained with the Scharer I method. Based on these results, it is recommended that the method be adopted as official, first action.

A new method of radioimmunoassay for human placental alkaline: Phosphatase

1972 ◽  
Vol 42 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Iino Shiro ◽  
Abe Kaoru ◽  
Oda Toshitugu ◽  
Suzuki Hiroshi ◽  
Sugiura Mamoru
Keyword(s):  
Alkaline Phosphatase ◽  
New Method ◽  
Placental Alkaline Phosphatase ◽  
Human Placental Alkaline Phosphatase

UTILIZATION OF PHENOLPHTHALEIN MONOPHOSPHATE TO TO DETERMINE THE PHOSPHATASE ACTIVITY OF MILK1

1972 ◽  
Vol 35 (7) ◽  
pp. 405-409 ◽  
Author(s):  
Dick H. Kleyn
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Rapid Method ◽  
Alkaline Phosphatase Activity ◽  
Collaborative Study ◽  
Correlation Coefficients ◽  
Raw Milk ◽  
New Method ◽  
Chocolate Milk ◽  
Positive Correlations

The properties of alkaline phosphatase as it exists in milk are considered. The more common methods of measuring alkaline phosphatase activity in milk are briefly reviewed, especially those employing substrates possessing “built-in” indicators which produce a chromogen directly upon hydrolysis. The visual procedure employing phenolphthalein monophosphate as the substrate is given. The sensitivity of this method is shown to be far greater than that of the Scharer I (Rapid) method. Results of an AOAC Collaborative study demonstrated that the method yields results that are as precise and either as accurate as or more accurate than those obtained by the Scharer I (Rapid) method. The quantitative spectrophotometric procedure employing the above substrate is presented. Comparison of this method with the Scharer modified spectrophotometric method on milk revealed correlation coefficients (Scharer method: 0.998 and modified new method 0.991) showing very little difference in the positive correlations of absorbance values and % raw milk. For chocolate milk, the values were 0.990 and 0.990 for the respective methods. Collaborative study of this method has demonstrated that the random error of the modified new method is almost twice that of the Scharer technique while the systematic error is only about one-fourth of the latter method.

scholarly journals A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26888-26894 ◽  
Author(s):  
Jeongmoo Lee ◽  
Seoyun Kim ◽  
Tae Hoon Kim ◽  
Seoung Ho Lee
Keyword(s):  
Aqueous Solution ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Sensor ◽  
Fluorescence Assay ◽  
Quantitative Detection ◽  
Ratiometric Fluorescence ◽  
Alp Activity

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

scholarly journals A New Method for the Quantitative Detection of Platelet Aggregates

1983 ◽  
Vol 11 (3) ◽  
pp. 531-534
Author(s):  
Michihiko HAYAKAWA ◽  
Satoru MIURA ◽  
Hidetoshi ENDO ◽  
Yoshio IWATA ◽  
Michitaka NAITO ◽  
...  
Keyword(s):  
New Method ◽  
Quantitative Detection ◽  
Platelet Aggregates
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