Determination of plasma antithrombin III by tanned red cell hemagglutination inhibition immunoassay

1983 ◽  
Vol 13 (2) ◽  
pp. 229-233
Author(s):  
Giuseppe M. Gandolfo ◽  
Maria Vittoria Torresi
Keyword(s):  
Hemagglutination Inhibition ◽  
Antithrombin Iii ◽  
Red Cell

Is Quantitative Determination of Fibrin(ogen) Degradation Products and Thrombin-Antithrombin III Complexes Useful to Diagnose Deep Venous Thrombosis in Outpatients?

1989 ◽  
Vol 62 (04) ◽  
pp. 1043-1045 ◽  
Author(s):  
Paul F M M van Bergen ◽  
Eduard A R Knot ◽  
Jan J C Jonker ◽  
Auke C de Boer ◽  
Moniek P M de Maat
Keyword(s):  
Quantitative Determination ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Antithrombin Iii ◽  
Degradation Products ◽  
Family Doctor ◽  
Diagnostic Value ◽  
Impedance Plethysmography ◽  
Fibrin Degradation Products

SummaryWe studied the diagnostic value of recently introduced ELISA’s for the determination of thrombin-antithrombin III (TAT) complexes, fibrin degradation products (FbDP), fibrinogen degradation products (FgDP) and total degradation products (TDP) for deep venous thrombosis (DVT) in plasma of 239 consecutive outpatients, suspected for DVT by their family doctor. DVT was confirmed by impedance plethysmography in 60 patients. Using the 95th percentile range of 42 healthy volunteers the sensitivity for the detection of DVT was: 37% for TAT, 95% for TDP, 92% for FbDP and 90% for FgDP. Specificity was: 88% for TAT, 16% for TDP, 20% for FbDP and 25% for FgDP.We conclude that these assays are of little value in the diagnosis of DVT in outpatients.

Determination of interdependent ligand effects on human red cell oxygen affinity

1982 ◽  
Vol 42 (4) ◽  
pp. 339-345
Author(s):  
W. G. Zijlstra ◽  
B. Oeseburg ◽  
G. Kwant ◽  
A. Zwart
Keyword(s):  
Oxygen Affinity ◽  
Red Cell ◽  
Ligand Effects

Response of breeding sows to long term dietary supplementation with folic acid

1994 ◽  
Vol 1994 ◽  
pp. 153-153
Author(s):  
P.B. Lynch ◽  
P.J.A. Sheehy
Keyword(s):  
Folic Acid ◽  
Dietary Supplementation ◽  
Red Cell ◽  
Restricted Feeding ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Soyabean Meal ◽  
Dietary Treatments

Dietary supplementation with folic acid has been shown to improve reproductive performance in sows (Lindemann 1993). However most studies have been for one cycle only and few have examined the effect of supplementation over several parities.One hundred and thirty four crossbred sows ranging in parity from 2 to 4 were selected at farrowing and randomly allocated to two dietary treatments of low and high supplemental folic acid (0 and 10 g per tonne, Roche Products Ltd.). Treatments were applied for the following three lactations and post weaning periods, two full pregnancies and to day 30 of the pregnancy following the third lactation. The diet fed contained barley, wheat, soyabean meal and meat and bone meal with nutrient levels of 14.0 MJ DE/kg and 1.02% lysine. Sows were individually penned throughout with restricted feeding in pregnancy (2.2 kg/day increasing to 2.5 kg/day in the final month), and ad libitum in lactation (approx 5.0 kg/day) and post weaning (approx 3.4 kg/day). Blood samples for determination of plasma and red cell folate were taken from 14 sows per treatment on days 4, 50 and 110 of one cycle. These were determined by a microbiological assay (modification of methods of Scott et al 1974 and Wilson and Home 1982).

scholarly journals Effect of oxalate and malonate on red cell metabolism

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1389-1393
Author(s):  
E Beutler ◽  
L Forman ◽  
C West
Keyword(s):  
Pyruvate Kinase ◽  
Cell Metabolism ◽  
Inhibitory Effect ◽  
Red Cells ◽  
Red Cell ◽  
Acid Analogue ◽  
2,3 Dpg

The addition of oxalate to blood stored in Citrate-phosphate-dextrose (CPD) produces a marked improvement in 2,3-diphosphoglycerate (2,3-DPG) preservation; an increase in 2,3-DPG levels can also be documented in short-term incubation studies. Oxalate is a potent in vitro inhibitor of red cell lactate dehydrogenase, monophosphoglycerate mutase, and pyruvate kinase (PK). In the presence of fructose 1,6-diphosphate the latter inhibitory effect is competitive with phospho(enol)pyruvate (PEP). Determination of the levels of intermediate compounds in red cells incubated with oxalate suggest the presence of inhibition at the PK step, indicating that this is the site of oxalate action. Apparent inhibition at the glyceraldehyde phosphate dehydrogenase step is apparently due to an increase in the NADH/NAD ratio. Oxalate had no effect on the in vivo viability of rabbit red cells stored in CPD preservatives for 21 days. Greater understanding of the toxicity of oxalate is required before it can be considered suitable as a component of preservative media, but appreciation of the mechanism by which it affects 2,3-DPG levels may be important in design of other blood additives. Malonate, the 3-carbon dicarboxylic acid analogue of oxalate late did not inhibit pyruvate kinase nor affect 2,3-DPG levels.

scholarly journals The use of photo-electric turbidometry in the determination of red cell counts, hematocrits, and hemoglobin

Blood ◽  
1947 ◽  
Vol 2 (6) ◽  
pp. 607-607
Author(s):  
J. H. WHITLOCK
Keyword(s):  
Title Page ◽  
Red Cell ◽  
Cell Counts

Abstract A communication from Dr. J. H. Whitlock (The use of photo-electric turbidometry in the determination of red cell counts, hematocrits, and hemoglobin. Blood 2: 463, September 1947) states that he has the D.V.M. and M.S., but not the Ph.D. as used erroneously after his name on the title page of the article.

Influence of temperature on blood pH of the human adult and newborn

1964 ◽  
Vol 19 (5) ◽  
pp. 897-900 ◽  
Author(s):  
Karlis Adamsons ◽  
Salha S. Daniel ◽  
Gillian Gandy ◽  
L. Stanley James
Keyword(s):  
Cell Concentration ◽  
Effect Of Temperature ◽  
Red Cell ◽  
Acid Base ◽  
Blood Ph ◽  
Influence Of Temperature On ◽  
The Mean ◽  
Ph Range ◽  
Two Populations

The effect of temperature upon pH of blood from adult and newborn humans was investigated. Although the mean ΔpH/ΔT values for the two populations differed, 0.0144/ °C for adults versus 0.0128 for the newborn, this was not peculiar to the source since the temperature-induced pH changes were identical when comparison was made between samples of similar pH and CO2 content. It was shown that even over the physiologic pH range ΔpH/ΔT is not constant but is a function of pH and CO2. No significant changes in ΔpH/ΔT were observed with oxygenation of hemoglobin. The influence of red cell concentration was detectable only when hematocrit values fell below 20%. A graph and an equation for the determination of ΔpH/ΔT of blood with a given pH and CO2 content is presented. acid base Submitted on December 9, 1963

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