Determination of Subtypic Specificities of HBSAg by Hemagglutination Inhibition and Radioimmunoassay

2015 ◽  
pp. 15-16
Author(s):  
Y. Miyakawa
Keyword(s):  
Hemagglutination Inhibition

scholarly journals The value of IgG to IgM ratio in predicting secondary dengue infection

2016 ◽  
Vol 46 (3) ◽  
pp. 113 ◽  
Author(s):  
I Putu Gede Karyana ◽  
Hendra Santoso ◽  
Bagus Ngurah Putu Arhana
Keyword(s):  
Prospective Study ◽  
Hemagglutination Inhibition ◽  
Good Predictor ◽  
Primary Infection ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Diagnostic Value ◽  
Post Test ◽  
A Prospective Study

Background The determination of primary or secondary dengueinfection using hemagglutination inhibition (HI) test is time-con-suming. The IgG to IgM ratio which can be obtained earlier wasused by several studies to differentiate secondary from primaryinfection, but they still reported various cut-off points.Objective To find the diagnostic value and best cut off point ofIgG to IgM ratio for predicting secondary dengue infection.Methods This was a prospective study carried out between July2003 and June 2004. Children with suspected dengue hemor-rhagic fever (DHF) were tested for HI during acute and convales-cent phase. The IgG and IgM titer were examined during the acutephase using ELISA method.Results Sixty-two children were recruited, 48 with secondary in-fection and 14 with primary infection. The prevalence of second-ary infection was 77%. The best cut off point of the IgG to IgM ratioto predict secondary infection was >1.1 with sensitivity of 87.5%,specificity 92.9%, likelihood ratio 12.3, and post test probability97.7%.Conclusion The IgG to IgM ratio of >1.1 is a good predictor forsecondary infection

scholarly journals THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION

1965 ◽  
Vol 121 (5) ◽  
pp. 793-806 ◽  
Author(s):  
Willard C. Schmidt ◽  
Dorothy J. Moore
Keyword(s):  
Hemagglutination Inhibition ◽  
Streptococcal Infection ◽  
Polysaccharide Antigen ◽  
Human Sera ◽  
Group A ◽  
Streptococcal Polysaccharide ◽  
Polysaccharide Antibody ◽  
Group A Streptococcal Polysaccharide

A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.

scholarly journals Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies

1979 ◽  
Vol 9 (6) ◽  
pp. 657-664
Author(s):  
B Forghani ◽  
N J Schmidt
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Sensitivity And Specificity ◽  
Hemagglutination Inhibition ◽  
Measles Virus ◽  
Complement Fixation ◽  
Infected Cell Culture ◽  
Viral Antigens ◽  
And Control

Enzyme immunoassay (EIA) systems for measles virus and rubella virus were studied from the standpoints of requirements for suitable viral antigens and control antigens, and the sensitivity and specificity of the tests for detecting antibody elicited by past infection (determination of immunity status), and for serodiagnosis of currenet infections. Crude or semipurified measles virus antigens were satisfactory for EIA, but antigens derived by pelleting virus from infected cell culture fluids were slightly more specific in their reactivity than were antigens produced from lysates of infected cells. However, reliable rubella EIA antigens could be produced only from infected cell culture fluids, and they required density gradient purification to render them suitably specific. Even with gradient-purified rubella antigens, it was necessary to use antigen prepared in an identical fashion from uninfected cell culture fluids as a control on the specificity of reactions obtained with test sera. With appropriate viral antigens and control antigens, both measles and rubella EIA systems were highly sensitive and specific for determination of immunity status and for serodiagnosis of current infections. Antibody was detectable earlier in the course of infection by EIA than by hemagglutination inhibition or complement fixation, but this did not limit the diagnostic value of the test, since titer increases demonstrable by EIA were usually greater than those detectable by hemagglutination inhibition or complement fixation tests.

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