Enzyme activity and electrophoretic pattern of isoenzymes of peroxidase, esterase and alkaline and acid phosphatase in relation to flowering inAmaranthus viridis L. - a quantitative SD plant

1981 ◽  
Vol 23 (5) ◽  
pp. 335-341 ◽  
Author(s):  
S. Sawhney ◽  
A. S. Basra ◽  
R. K. Kohli
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Electrophoretic Pattern ◽  
Alkaline And Acid Phosphatase

Comparison of eight Pisolithustinctorius isolates for growth rate, enzyme activity, and phytohormone production

1987 ◽  
Vol 17 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Growth Rate ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Indoleacetic Acid ◽  
The Other ◽  
Northern California ◽  
Isozyme Patterns ◽  
Gibberellin Production ◽  
Alkaline And Acid Phosphatase

Eight isolates of Pisolithustinctorius (Pers.) Coker and Couch (three each from Georgia and northern California and one each from Oregon and Washington) were compared invitro for growth rate, for alkaline and acid phosphatase and nitrate reductase activities, for acid phosphatase isozyme patterns, and for cytokinin, indoleacetic acid, and gibberellin production. Significant differences appeared between isolates for each parameter examined. All isolates showed relatively low phosphatase and nitrate reductase activities. Isolate S-359 from northern California grew the slowest in culture and produced significantly more indoleacetic acid than all other isolates and more cytokinin than six of the other seven isolates; this isolate was also the only one of the eight that did not share at least one acid phosphatase allele with the others in the isozyme analysis.

Rat prostatic acid phosphatase: androgenic control of isoelectric focusing patterns

1983 ◽  
Vol 61 (7) ◽  
pp. 744-749 ◽  
Author(s):  
J. Downey ◽  
D. Mahan ◽  
T. G. Flynn ◽  
C. E. Bird ◽  
A. F. Clark
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Isoelectric Focusing ◽  
Specific Activity ◽  
Electrophoretic Pattern ◽  
Prostatic Acid Phosphatase ◽  
Staining Intensity ◽  
Adult Rats ◽  
Enzyme Specific Activity ◽  
Ph Range

To further characterize the androgen dependence of prostatic acid phosphatase (AP), the isoelectric focusing patterns of enzyme activity have been examined for normal and castrated adult rats and for rats receiving androgen injections. Isoelectric focusing was performed in polyacrylamide gels over the pH range 4–8. Naphthyl phosphate was used as substrate for staining. For normal rats there was a single lysosomal band (isoelectric point (pI) = 7.35 ± 0.04), four closely migrating secretory bands (pI = 5.96–5.63), and an androgen-dependent band (pI = 6.37 ± 0.05) which as yet has not been identified as either lysosomal or secretory. Following castration the secretory bands decreased significantly in staining intensity, the androgen-dependent band disappeared, and two new lysosomal bands (pI's = 7.13 ± 0.03 and 7.00 ± 0.03) appeared. With androgen replacement the latter two bands disappeared, the androgen-dependent band reappeared, and the secretory bands increased in staining intensity but with the most anodic of the four appearing before the others. This suggests that it could be a precursor to the others. The isoelectric focusing patterns of AP activity appear to be a better method of assessing the androgen status of the prostate than are the previously used parameters, namely, enzyme specific activity, degree of inhibition by tartrate, and polyacrylamide gel electrophoretic pattern.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

Alteration of adenoid tissue alkaline and acid phosphatase in children with secretory otitis media

2006 ◽  
Vol 70 (6) ◽  
pp. 1069-1076 ◽  
Author(s):  
Snezana Jesic ◽  
Ljuba Stojiljkovic ◽  
Zeljko Petrovic ◽  
Vladimir Djordjevic ◽  
Vladimir Nesic ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Otitis Media ◽  
Secretory Otitis Media ◽  
Adenoid Tissue ◽  
Alkaline And Acid Phosphatase

scholarly journals Enzyme Histochemical Studies of Adipose Tissue in Porcine Yellow Fat Disease

1974 ◽  
Vol 11 (6) ◽  
pp. 465-476 ◽  
Author(s):  
L. H. J. C. Danse ◽  
W. A. Steenbergen-Botterweg
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Acid Phosphatase ◽  
Nonspecific Esterase ◽  
Cell Structures ◽  
Increased Activity

Adipose tissue of piglets with yellow fat disease had increased activity of nonspecific esterase, 5-nucleotidase, and acid phosphatase. Since these enzymes are associated with different cell structures and damage to these structures can result in increased enzyme activity, they are criteria for pathogenetic study of yellow fat disease.

An Investigation of the Histochemical Technique for the Localization of Acid Phosphomonoesterase

1950 ◽  
Vol s3-91 (15) ◽  
pp. 315-330
Author(s):  
FRANCES MACDONALD
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Standard Technique ◽  
Formalin Fixation ◽  
Histochemical Technique ◽  
Acid Phosphomonoesterase

1. A method is described for assessing the depth of ‘staining’ obtained with the acid phosphatase technique and a detailed scheme is given of the standard technique used. 2. It is concluded that the technique specifically demonstrates the activity of acid phosphatase, since ‘staining’ is abolished in the absence of substrate, in heated sections, and in the presence of fluoride. 3. An investigation has been carried out to determine the extent to which the reaction is affected by altering various stages in the technique. 4. The effect of formalin fixation on the reaction has been investigated. 5. It has been shown that sites in the rabbit medulla having an affinity for the reagents used in the technique differ from the sites at which a precipitate is deposited as a result of enzyme activity. 6. Evidence is presented suggesting that the technique may not demonstrate the true physiological localization of the enzyme. 7. It is suggested that the technique may be of value as a neurohistological method.

The Cytoplasmic Cytology of Sarcoma 180

1952 ◽  
Vol s3-93 (24) ◽  
pp. 413-426
Author(s):  
LEONARD G. WORLEY ◽  
HERMAN W. SPATER
Keyword(s):  
Ascorbic Acid ◽  
Acid Phosphatase ◽  
Ribonucleic Acid ◽  
Living Cell ◽  
Sarcoma 180 ◽  
Golgi Bodies ◽  
Alkaline And Acid Phosphatase ◽  
Golgi Network ◽  
Golgi Zone ◽  
Large Spherical

Typical sarcoma 180 cells present a large spherical lipoid or Golgi zone adjacent to the nucleus within which discrete, homogeneous and vesicular, Golgi bodies (lipochondria) are distributed. No Golgi network can be detected in the living cell, but such networks may be produced artificially by means of hypertonic solutions and by shrinking vitally stained cells. All of the cytoplasm, except the lipoid zone, is strongly basiphil owing to the presence of ribonucleic acid. Mitochondria, mostly rod shaped, form a halo of variable width surrounding the lipoid zone except adjacent to the nucleus. These lie partly within the ribonucleic acid containing cytoplasm. Tests for glycogen, alkaline, and acid phosphatase, and for lipase are largely negative. A positive reaction to Bourne's test for ascorbic acid is given by many of the lipochondria.

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