Comparison of eight Pisolithustinctorius isolates for growth rate, enzyme activity, and phytohormone production

1987 ◽  
Vol 17 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Growth Rate ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Indoleacetic Acid ◽  
The Other ◽  
Northern California ◽  
Isozyme Patterns ◽  
Gibberellin Production ◽  
Alkaline And Acid Phosphatase

Eight isolates of Pisolithustinctorius (Pers.) Coker and Couch (three each from Georgia and northern California and one each from Oregon and Washington) were compared invitro for growth rate, for alkaline and acid phosphatase and nitrate reductase activities, for acid phosphatase isozyme patterns, and for cytokinin, indoleacetic acid, and gibberellin production. Significant differences appeared between isolates for each parameter examined. All isolates showed relatively low phosphatase and nitrate reductase activities. Isolate S-359 from northern California grew the slowest in culture and produced significantly more indoleacetic acid than all other isolates and more cytokinin than six of the other seven isolates; this isolate was also the only one of the eight that did not share at least one acid phosphatase allele with the others in the isozyme analysis.

Acid phosphatase, alkaline phosphatase, and nitrate reductase activity of selected ectomycorrhizal fungi

1989 ◽  
Vol 67 (3) ◽  
pp. 750-753 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Phosphatase Activity ◽  
Ectomycorrhizal Fungi ◽  
Acid Phosphatase Activity ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Phosphatase Alkaline

Seventeen isolates, encompassing five genera and eight species of ectomycorrhizal fungi, were compared for acid phosphatase, alkaline phosphatase, and nitrate reductase activity. Isolates within species differed in enzyme activity and isozyme patterns by host specificity and site (as exemplified by the genus Suillus). Host and site may have affected phosphatase enzyme activity. Generally, the Douglas-fir associates, which dominate in mesic sites, have higher acid phosphatase activity than pine associates, which mostly occupy xeric sites; however, pine associates from mesic sites also have higher acid phosphatase activity (e.g., S. tomentosus). In four isolates of Amanita muscaria, the effect of site was also apparent. Two of them, which have significantly higher acid phosphatase activity than the others, were isolated from mesic sites. The isozyme pattern of the genus Suillus appeared to be separated by host groups. Other isolates with only one species also differed more or less by host groups. They shared at least one band within host groups, except for the two isolates of Paxillus involutus from different hosts. The P. involutus S-403 isolated from an orchard showed much higher nitrate reductase activity than all other isolates. No apparent differences in nitrate reductase activity were found between the other isolates.

Enzyme activity and phytohormone production of a mycorrhizal fungus, Laccarialaccata

1987 ◽  
Vol 17 (8) ◽  
pp. 855-858 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Mycorrhizal Fungi ◽  
Reductase Activity ◽  
Mycorrhizal Fungus ◽  
High Elevation ◽  
The Other ◽  
Forest Nursery ◽  
Natural Forests ◽  
Polymorphic Gene

Six isolates of Laccarialaccata, S-167 from a forest nursery and S-238, S-283, S-326, S-444, and S-472 from natural forests, were analyzed for acid phosphatase, alkaline phosphatase, and nitrate reductase activity, acid phosphatase isozyme patterns, and IAA and cytokinin production. Differences in enzyme activity and phytohormone production were prominent among the isolates. The patterns of acid phosphatase isozyme could be clearly divided into three host-related groups. Two polymorphic gene loci could be identified as coding for enzymes of acid phosphatase. Two of these gene loci, Acp-b and Acp-c, are characterized by mostly constant, host-dependent frequencies. The other, Acp-d, exhibits allele frequencies related to different habitats. Five isolates share the same Acp-a habitat. Four isolates share the Acp-b habitat, two the Acp-c habitat, and only one isolate, S-238, was from a high elevation at the Acp-d habitat. The isolate from a forest nursery differed strikingly in several characteristics from the other isolates, all of which were from a natural forest. This suggests that nursery soilmanagement practices may select for particular edaphic ecotypes of mycorrhizal fungi.

scholarly journals POINSETTIA CULTIVARS RESPOND DIFFERENTLY TO MOLYBDENUM DEFICIENCY

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1086c-1086
Author(s):  
Douglas A. Cox
Keyword(s):  
Trace Elements ◽  
Enzyme Activity ◽  
Nitrate Reductase ◽  
Trace Element ◽  
Calcium Nitrate ◽  
Potassium Nitrate ◽  
The Other ◽  
Sphagnum Peat ◽  
Foliar Symptoms ◽  
Fertilizer Solutions

Nine cultivars were grown in a 1:1 sphagnum peat and perlite medium with no limestone or trace element fertilizer. Fertilizer solutions of 300 ppm N and K (calcium nitrate and potassium nitrate) and 24 ppm Mg were applied at every watering. Solutions supplied all trace elements and either 0 or 1 ppm Mo. Moderate to severe foliar symptoms of Mo deficiency developed on `Annette Hegg Brilliant Diamond' and `Eckespoint Lilo' with 0 ppm Mo. Symptoms did not occur with 1 ppm Mo. No Mo deficiency symptoms developed on the other 7 cultivars which included `Supjibi', `Gutbier V-17 Angelika', `Peace Regal Velvet', and `Cheers!'. With 0 ppm Mo these cultivars generally maintained higher levels of nitrate reductase enzyme activity and lower tissue nitrate levels than the 2 showing symptoms.

scholarly journals Growth and endoglucanase activity of Acetivibrio cellulolyticus grown in three different cellulosic substrates

1999 ◽  
Vol 30 (4) ◽  
pp. 310-314 ◽  
Author(s):  
Cássia Regina Sanchez ◽  
Clarita Schvartz Peres ◽  
Heloiza Ramos Barbosa
Keyword(s):  
Growth Rate ◽  
Enzyme Activity ◽  
Growth Kinetics ◽  
Specific Growth ◽  
Carbon Sources ◽  
The Other ◽  
Crystalline Cellulose ◽  
Amorphous Cellulose ◽  
Endoglucanase Activity ◽  
Kinetics Of

The growth kinetics of Acetivibrio cellulolyticus grown in medium containing different carbon sources (cellobiose, amorphous or crystalline cellulose) was investigated. The specific growth rate was higher in cellobiose fed cultures than in the presence of the other two substrates. Endoglucanase production was greater in cultures grown on amorphous cellulose; enzyme activity increased during the stationary phase in cultures grown on crystalline cellulose.

Growth and Performance of Agriculture Sector in India

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Naresh Singla ◽  
Mamandeep Kaur
Keyword(s):  
Growth Rate ◽  
Capital Formation ◽  
The Other ◽  
Agricultural Credit ◽  
Agricultural Growth ◽  
Indian Economy ◽  
Agriculture Sector ◽  
Food Grain ◽  
And Performance ◽  
Agricultural Labourers

The growth of agriculture and allied sectors is critical for the Indian economy as about 49 percent of the population is directly or indirectly dependent on agriculture. During the last decade and so, the agriculture sector has undergone profound changes resulting in sharp deceleration in its growth. The study has attempted to analyze growth and performance of the agriculture sector in India since 1980-81 and tries to comprehend some of the factors responsible for the deceleration in growth. The study has shown that agriculture sector has been able to show tremendous improvement in expansion of area and production of food grain and non-food grain crops. However, there are so many underlying factors responsible for slowdown of the agricultural growth. Some of the factors identified include: Increase in area under non-agriculture uses, excessive dependence on rain fed farming, increase in number of agricultural labourers, reducing size of the operation holdings, over use of agri-inputs, inequity in the distribution of agriculture credit along with sharp deceleration in public gross capital formation in agriculture etc. The study pointed in order to achieve higher growth rate, there is a need to enhance the gross capital formation in agriculture sector particularly on irrigation so that more area can be brought under assured irrigation. Bringing equity in distribution of agricultural credit coupled with judicious and need-based agricultural inputs are some of the other recommendations drawn based upon the study.

A polycentric growth model of gallium arsenide epitaxial layers from the gas phase with simultaneous diffusion, adsorption and chemical reaction

1988 ◽  
Vol 53 (12) ◽  
pp. 2995-3013
Author(s):  
Emerich Erdös ◽  
Jindřich Leitner ◽  
Petr Voňka ◽  
Josef Stejskal ◽  
Přemysl Klíma
Keyword(s):  
Growth Rate ◽  
Gallium Arsenide ◽  
Epitaxial Growth ◽  
Gas Phase ◽  
Surface Reaction ◽  
Quantitative Description ◽  
The Other ◽  
Rate Equations ◽  
Impact Interaction ◽  
Partial Pressures

For a quantitative description of the epitaxial growth rate of gallium arsenide, two models are proposed including two rate controlling steps, namely the diffusion of components in the gas phase and the surface reaction. In the models considered, the surface reaction involves a reaction triple - or quadruple centre. In both models three mechanisms are considered which differ one from the other by different adsorption - and impact interaction of reacting particles. In every of the six cases, the pertinent rate equations were derived, and the models have been confronted with the experimentally found dependences of the growth rate on partial pressures of components in the feed. The results are discussed with regard to the plausibility of individual mechanisms and of both models, and also with respect to their applicability and the direction of further investigations.

Use of monoclonal and polyclonal antibodies to detect prostatic acid phosphatase by immunoblotting.

1986 ◽  
Vol 32 (10) ◽  
pp. 1832-1835 ◽  
Author(s):  
P C Patel ◽  
L Aubin ◽  
J Côte
Keyword(s):  
Monoclonal Antibodies ◽  
Acid Phosphatase ◽  
Western Blot ◽  
Polyclonal Antibodies ◽  
Prostatic Acid Phosphatase ◽  
The Other ◽  
Dot Blot ◽  
Western Blots ◽  
Dot Blot Assay ◽  
Polyclonal Antisera

Abstract We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.

Sign in / Sign up

Export Citation Format

Share Document