Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

The properties of acidic compartments in developing schistosomula ofSchistosoma mansoni

Parasitology ◽  
2003 ◽  
Vol 127 (3) ◽  
pp. 253-264 ◽  
Author(s):  
B. H. AL-ADHAMI ◽  
J. THORNHILL ◽  
A. AKHKHA ◽  
M. J. DOENHOFF ◽  
J. R. KUSEL
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Histochemical Staining ◽  
Transformation Process ◽  
Percentage Area ◽  
Red Dye ◽  
Energy Dependent ◽  
Acidic Compartments ◽  
Phosphatase Enzyme

A variety of fluorescent probes have been used to study the acidic compartments in cercariae and schistosomula ofSchistosoma mansoni. Freshly transformed schistosomula treated with the LysoTracker Red dye specific for lysosomes showed large acid-containing compartments (0·5–10 μm in size). The uptake of the dye is an energy-dependent process that depends on the metabolic activity of schistosomula. The compartments were quantified individually with respect to area, quantity of fluorescence and the total number/schistosomulum. Under normal conditions these compartments were not found in untreated cercariae, but appeared in cercariae slightly damaged by poly-L-lysine. The formation of these compartments seemed to be related to the development of cercariae into schistosomula as the number of compartments and uptake of fluorescence increased with time after transformation. Also, the method of transformation as well as thein vitroincubation of the parasite affected the percentage area of compartments/schistosomulum. Acid phosphatase enzyme activity was assessed using an endogenous phosphatase probe. Living and fixed schistosomula displayed the presence of enzyme activity in compartments of the same size and distribution as the acid-rich compartments. This was confirmed by histochemical staining showing deposition of enzyme-generated lead at the sites of phosphatase activity. We suggest that the development of acidic compartments is important during the transformation process or as a consequence of damage.

scholarly journals Acid Phosphatase Enzyme Activity in Mouse Kidney Studied by X-Ray Microanalysis

2006 ◽  
Vol 12 (S02) ◽  
pp. 238-239
Author(s):  
M Bernal
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Mouse Kidney ◽  
X Ray ◽  
Phosphatase Enzyme

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2006

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