Computer-assisted microscope analysis of feulgen-stained nuclei in gonadotroph adenomas and null-cell adenomas of the pituitary gland

1997 ◽  
Vol 8 (2) ◽  
pp. 109-120 ◽  
Author(s):  
M. Beatriz S. Lopes ◽  
Isabelle Salmon ◽  
Nathalie Nagy ◽  
Christine Decaestecker ◽  
Jean-Lambert Pasteels ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Computer Assisted ◽  
Null Cell ◽  
Microscope Analysis ◽  
Null Cell Adenomas

Null cell adenomas of the pituitary gland. An immunohistochemical study

1989 ◽  
Vol 158 (3) ◽  
pp. 213-217 ◽  
Author(s):  
Ruth Holm ◽  
Jahn M. Nesland ◽  
Arne Attramadal ◽  
Johan Halse ◽  
Jan V. Johannessen
Keyword(s):  
Pituitary Gland ◽  
Immunohistochemical Study ◽  
Null Cell ◽  
Null Cell Adenomas

Null Cell Adenomas of the Pituitary Gland: an Institutional Review of Their Clinical Imaging and Behavioral Characteristics

2014 ◽  
Vol 26 (1) ◽  
pp. 63-70 ◽  
Author(s):  
James A. Balogun ◽  
Eric Monsalves ◽  
Kyle Juraschka ◽  
Kashif Parvez ◽  
Walter Kucharczyk ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Behavioral Characteristics ◽  
Null Cell ◽  
Clinical Imaging ◽  
Institutional Review ◽  
Null Cell Adenomas

Immunonegative "Null Cell" Adenomas and Gonadotropin (Gn) Subunit (SUs) Immunopositive Adenomas Share Frequent Expression of Multiple Transcription Factors

2006 ◽  
Vol 17 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Yudo Ishii ◽  
Masanori Suzuki ◽  
Susumu Takekoshi ◽  
Noboru Egashira ◽  
Michio Yamazaki ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Null Cell ◽  
Null Cell Adenomas

Null cell adenomas, oncocytomas, and gonadotroph adenomas of the human pituitary: An immunocytochemical and ultrastructural anafysis of 300 cases

1993 ◽  
Vol 4 (1) ◽  
pp. 20-27 ◽  
Author(s):  
George Kontogeorgos ◽  
Kalman Kovacs ◽  
Eva Horvath ◽  
Bernd W. Scheithauer
Keyword(s):  
Null Cell ◽  
Human Pituitary ◽  
Null Cell Adenomas

scholarly journals Line Scan Raman Microspectroscopy for Label-Free Diagnosis of Human Pituitary Biopsies

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3577 ◽  
Author(s):  
Daniela Bovenkamp ◽  
Alexander Micko ◽  
Jeremias Püls ◽  
Fabian Placzek ◽  
Romana Höftberger ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Pituitary Adenomas ◽  
Nearest Neighbor ◽  
Principal Component ◽  
Raman Microspectroscopy ◽  
Label Free ◽  
K Nearest Neighbor ◽  
Null Cell ◽  
Human Pituitary ◽  
Line Scan

Pituitary adenomas are neoplasia of the anterior pituitary gland and can be subdivided into hormone-producing tumors (lactotroph, corticotroph, gonadotroph, somatotroph, thyreotroph or plurihormonal) and hormone-inactive tumors (silent or null cell adenomas) based on their hormonal status. We therefore developed a line scan Raman microspectroscopy (LSRM) system to detect, discriminate and hyperspectrally visualize pituitary gland from pituitary adenomas based on molecular differences. By applying principal component analysis followed by a k-nearest neighbor algorithm, specific hormone states were identified and a clear discrimination between pituitary gland and various adenoma subtypes was achieved. The classifier yielded an accuracy of 95% for gland tissue and 84–99% for adenoma subtypes. With an overall accuracy of 92%, our LSRM system has proven its potential to differentiate pituitary gland from pituitary adenomas. LSRM images based on the presence of specific Raman bands were created, and such images provided additional insight into the spatial distribution of particular molecular compounds. Pathological states could be molecularly differentiated and characterized with texture analysis evaluating Grey Level Cooccurrence Matrices for each LSRM image, as well as correlation coefficients between LSRM images.

The immunohistochemical and radiological features of nonfunctioning pituitary adenomas

2015 ◽  
Vol 61 (6) ◽  
pp. 4-9 ◽  
Author(s):  
Anna Konstantinovna Lipatenkova ◽  
Larisa Konstantinovna Dzeranova ◽  
Ekaterina Aleksandrovna Pigarova ◽  
Nadezhda Sergeevna Dalantaeva ◽  
Ljudmila Igorevna Astaf'eva ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Area Under The Curve ◽  
Pituitary Hormones ◽  
Serum Markers ◽  
Invasive Growth ◽  
Null Cell ◽  
Ki 67 ◽  
Nonfunctioning Pituitary Adenomas ◽  
Radiological Features ◽  
Null Cell Adenomas

Pituitary adenomas without clinically active hypersecretion are summarized under the term nonfunctioning pituitary adenomas (NFPAs). Since there are no specific serum markers, the differential diagnosis and treatment imply special difficulties.Aim.To investigate the immunohistochemical and radiological features of NFPAs and assess the granins — chromogranin A (CgA), secretogranin II (SgII), secretoneurin (Sn) as immunohistochemical markers of NFPAs.Matherial and methods.50 pituitary adenomas excised surgically were immunostained to reveal pituitary hormones, ki-67, CgА. SgII, Sn. All patients underwent MRI, invasive growth was estimated due to J.Hardy classification (1973).Results.24 (51,1%) were gonadotropic tumors, 12 (25,5%) — null cell adenomas. Immunopositivity for ACTH was determined in 6 cases (12,7%), for GH in 5 (10,6%) cases, for PRL in 4 (8,5%). The median level of ki-67 was 2% (min. — 0.5%, max. — 7%). The CgA, SgII, Sn immunopositivity was found in 83, 93,6, 85,1% respectively, being more expressed in gonadotropinomas and null cell adenomas. Invasive growth was detected in 28 (44%) cases, among the invasive adenomas 22 tumors were giant. CgA expression is adverse prognostic factor, area under the curve (AUC) with 0,705. We did not find any correlation between ACTH-, STH-, CgII- and Sn- immunopositivity, ki-67 and invasive growth.Conclusions.Our work shows that a majority of NFPAs are truly secreting adenomas with significant numbers comprising potentially hazardous cortico- and somatotropinomas. CgA, SgII and Sn have a high expression in most of the NFPAs.

scholarly journals Intermediate Filaments in the Human Pituitary Gland: An Immunohistochemical Study

1990 ◽  
Vol 17 (2) ◽  
pp. 131-136 ◽  
Author(s):  
W.C. Halliday ◽  
S.L. Asa ◽  
K. Kovacs ◽  
B.W. Scheithauer
Keyword(s):  
Pituitary Gland ◽  
Intermediate Filaments ◽  
Immunohistochemical Study ◽  
Serial Sections ◽  
Null Cell ◽  
Human Pituitary ◽  
Human Pituitary Gland ◽  
Immunohistochemical Techniques ◽  
Corticotroph Adenomas ◽  
Small Subpopulation

ABSTRACT:Normal pituitaries, pituitaries showing CHC and selected adenomas were assessed for their possible content of intermediate filaments (IF), using immunohistochemical techniques. Normal pituitaries were stained for each of the five known IF (Cytokeratins (CK), glial fibrillary acidic protein (GFAP), desmin, vimentin, and neurofilaments (NF)). Desmin could not be demonstrated, and NF staining was limited to processes in the posterior gland. In serial sections, CK was demonstrated in some corticotrophs, somatotrophs, and lactotrophs. Vimentin was limited to processes in the posterior gland. Folliculo-stellate cells (FSC), demonstrated by staining for SlOO protein, exhibited a small subpopulation staining for CK, and another subpopulation staining for GFAP. GFAP was also demonstrated in glial processes in the posterior gland. Cells showing Crooke's hyaline change stained intensely for CK. Null cell adenomas did not stain for CK. Corticotroph adenomas, somatotroph adenomas, with or without fibrous bodies, and lactotroph adenomas contain CK-immunoreactive cells. We conclude that CK is the major IF present in the anterior pituitary gland, within some normal corticotrophs, somatotrophs and lactotrophs, and also in adenomas derived from these cells.

Clinical, pathologic, and imaging characteristics of pituitary null cell adenomas as defined according to the 2017 World Health Organization criteria: a case series from two pituitary centers

Pituitary ◽  
2019 ◽  
Vol 22 (5) ◽  
pp. 514-519 ◽  
Author(s):  
Joao Paulo Almeida ◽  
Corbin C. Stephens ◽  
Jennifer M. Eschbacher ◽  
Michelle M. Felicella ◽  
Kevin C. J. Yuen ◽  
...  
Keyword(s):  
World Health Organization ◽  
Case Series ◽  
World Health ◽  
Null Cell ◽  
Imaging Characteristics ◽  
Health Organization ◽  
Null Cell Adenomas
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