Immunonegative "Null Cell" Adenomas and Gonadotropin (Gn) Subunit (SUs) Immunopositive Adenomas Share Frequent Expression of Multiple Transcription Factors

2006 ◽  
Vol 17 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Yudo Ishii ◽  
Masanori Suzuki ◽  
Susumu Takekoshi ◽  
Noboru Egashira ◽  
Michio Yamazaki ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Null Cell ◽  
Null Cell Adenomas

Null cell adenomas, oncocytomas, and gonadotroph adenomas of the human pituitary: An immunocytochemical and ultrastructural anafysis of 300 cases

1993 ◽  
Vol 4 (1) ◽  
pp. 20-27 ◽  
Author(s):  
George Kontogeorgos ◽  
Kalman Kovacs ◽  
Eva Horvath ◽  
Bernd W. Scheithauer
Keyword(s):  
Null Cell ◽  
Human Pituitary ◽  
Null Cell Adenomas

scholarly journals SUN-135 Characterization of Transcription Factor Immunostaining and Null Cell Adenoma Status in Hormone Negative Pituitary Adenomas

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Hans C Baertsch ◽  
Dhiraj J Pangal ◽  
Trey Garrett ◽  
Andrew Brunswick ◽  
Martin Rutkowski ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Pituitary Adenomas ◽  
Vision Loss ◽  
World Health ◽  
Tumor Diameter ◽  
Null Cell ◽  
Cell Adenoma ◽  
Cavernous Sinus Invasion ◽  
Endocrine Organs

Abstract Introduction: Since the 2017 W.H.O. classification of pituitary adenomas redefined null cell adenomas (NCAs) as negative for all adenohypophyseal hormones and the transcription factors (TFs) SF-1, PIT-1, and T-PIT, limited data exist characterizing these tumors1. We characterize NCAs in comparison to hormone negative adenomas (HNAs), which demonstrate negative hormone immunostaining in the context of positive TF immunoreactivity. Methods: Retrospective review of 22 patients with HNAs between 2011-2019. Samples were stained for PIT-1 and SF-1. Negative ACTH staining served as a proxy for T-PIT given demonstrated prior concordance of these stains2. Demographics, tumor characteristics, preoperative symptoms, and postoperative outcomes were assessed. Results: Fifteen samples (68%) stained negative for both PIT-1 and SF-1 and were classified as NCAs. Seven were positive for SF-1 (n=3), PIT-1 (n=3), or both (n=1) and were classified as HNAs. NCA patients were predominantly female (80%), while those with HNAs were predominantly male (57%). All tumors were macroadenomas, with mean maximal tumor diameter of 28mm in NCAs vs 23mm in HNAs (p=0.2705). NCAs were more likely to demonstrate suprasellar invasion (100% vs. 71%, p=0.0325), and although not statistically significant, cavernous sinus invasion (53% vs. 43%, p=0.6695), and higher MIB-1 proliferative index (2.271 vs. 1.971, p=0.733). The most common preoperative symptoms were headache (73% NCA, 71% HNA) and vision loss (53%, 40%). Postoperative improvements in headache (60% NCA, 71% HNA) and vision (53%, 50%) were comparable. Sixty-four percent of NCAs underwent gross total resection vs. 43% of HNAs (p=.3712). There were no recurrences or progressions in either group over 24mo. Few comparisons reached significance, potentially due to limited sample size. Conclusion: A majority of HNAs demonstrated negative TF immunostaining and met criteria for NCAs. NCAs may be more common in females and demonstrate more suprasellar invasion than HNAs, but otherwise, do not vary significantly. TF staining may be of limited clinical utility in identifying high-risk pathology, however future studies with larger cohorts are warranted. References: 1. Osamura RY, Lopes MBS, Grossman A, Matsuno A, Korbonits M, Trouillas J, Kovacs K (2017) Pituitary adenoma. In: Lloyd RV, Osamura RY, Klöppel G, Rosai J (eds) World health organization classification of tumours of endocrine organs, 4th edn. IARC, Lyon, pp 14-18. 2. Nishioka H, Inoshita N, Mete O, Asa SL, Hayashi K, Takeshita A, Fukuhara N, Yamaguchi-Okadad M, Takeuchi Y, Yamada S (2015) The Complementary Role of Transcription Factors in the Accurate Diagnosis of Clinically Nonfunctioning Pituitary Adenomas. Endocr Path 26(4):249-55.

The immunohistochemical and radiological features of nonfunctioning pituitary adenomas

2015 ◽  
Vol 61 (6) ◽  
pp. 4-9 ◽  
Author(s):  
Anna Konstantinovna Lipatenkova ◽  
Larisa Konstantinovna Dzeranova ◽  
Ekaterina Aleksandrovna Pigarova ◽  
Nadezhda Sergeevna Dalantaeva ◽  
Ljudmila Igorevna Astaf'eva ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Area Under The Curve ◽  
Pituitary Hormones ◽  
Serum Markers ◽  
Invasive Growth ◽  
Null Cell ◽  
Ki 67 ◽  
Nonfunctioning Pituitary Adenomas ◽  
Radiological Features ◽  
Null Cell Adenomas

Pituitary adenomas without clinically active hypersecretion are summarized under the term nonfunctioning pituitary adenomas (NFPAs). Since there are no specific serum markers, the differential diagnosis and treatment imply special difficulties.Aim.To investigate the immunohistochemical and radiological features of NFPAs and assess the granins — chromogranin A (CgA), secretogranin II (SgII), secretoneurin (Sn) as immunohistochemical markers of NFPAs.Matherial and methods.50 pituitary adenomas excised surgically were immunostained to reveal pituitary hormones, ki-67, CgА. SgII, Sn. All patients underwent MRI, invasive growth was estimated due to J.Hardy classification (1973).Results.24 (51,1%) were gonadotropic tumors, 12 (25,5%) — null cell adenomas. Immunopositivity for ACTH was determined in 6 cases (12,7%), for GH in 5 (10,6%) cases, for PRL in 4 (8,5%). The median level of ki-67 was 2% (min. — 0.5%, max. — 7%). The CgA, SgII, Sn immunopositivity was found in 83, 93,6, 85,1% respectively, being more expressed in gonadotropinomas and null cell adenomas. Invasive growth was detected in 28 (44%) cases, among the invasive adenomas 22 tumors were giant. CgA expression is adverse prognostic factor, area under the curve (AUC) with 0,705. We did not find any correlation between ACTH-, STH-, CgII- and Sn- immunopositivity, ki-67 and invasive growth.Conclusions.Our work shows that a majority of NFPAs are truly secreting adenomas with significant numbers comprising potentially hazardous cortico- and somatotropinomas. CgA, SgII and Sn have a high expression in most of the NFPAs.

Clinical, pathologic, and imaging characteristics of pituitary null cell adenomas as defined according to the 2017 World Health Organization criteria: a case series from two pituitary centers

Pituitary ◽  
2019 ◽  
Vol 22 (5) ◽  
pp. 514-519 ◽  
Author(s):  
Joao Paulo Almeida ◽  
Corbin C. Stephens ◽  
Jennifer M. Eschbacher ◽  
Michelle M. Felicella ◽  
Kevin C. J. Yuen ◽  
...  
Keyword(s):  
World Health Organization ◽  
Case Series ◽  
World Health ◽  
Null Cell ◽  
Imaging Characteristics ◽  
Health Organization ◽  
Null Cell Adenomas

Computer-assisted microscope analysis of feulgen-stained nuclei in gonadotroph adenomas and null-cell adenomas of the pituitary gland

1997 ◽  
Vol 8 (2) ◽  
pp. 109-120 ◽  
Author(s):  
M. Beatriz S. Lopes ◽  
Isabelle Salmon ◽  
Nathalie Nagy ◽  
Christine Decaestecker ◽  
Jean-Lambert Pasteels ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Computer Assisted ◽  
Null Cell ◽  
Microscope Analysis ◽  
Null Cell Adenomas

Gsα, protein kinase C, cathepsin D, growth factors, estrogen receptor-related protein, and p53 in prolactin cell adenomas and null cell adenomas of the pituitary

1998 ◽  
Vol 9 (2) ◽  
pp. 135-148
Author(s):  
Lars Wellhausen ◽  
Wolfgang Saeger ◽  
Wolf Müller ◽  
Michael Derwahl ◽  
Christiane Hamacher ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Protein Kinase C ◽  
Growth Factors ◽  
Protein Kinase ◽  
Cathepsin D ◽  
Related Protein ◽  
Null Cell ◽  
Prolactin Cell ◽  
Kinase C ◽  
Null Cell Adenomas

Inactivation of the p16 Gene in Human Pituitary Nonfunctioning Tumors by Hypermethylation Is More Common in Null Cell Adenomas

2001 ◽  
Vol 12 (3) ◽  
pp. 281-290 ◽  
Author(s):  
Katharina H Ruebel ◽  
Long Jin ◽  
Shuya Zhang ◽  
Bernd W Scheithauer ◽  
Ricardo V Lloyd
Keyword(s):  
Null Cell ◽  
Human Pituitary ◽  
P16 Gene ◽  
Null Cell Adenomas

Gonadotropin Secretion in Vitro by Human Pituitary Null Cell Adenomas and Oncocytomas**

1986 ◽  
Vol 62 (5) ◽  
pp. 1011-1019 ◽  
Author(s):  
SYLVIA L. ASA ◽  
BLAIR M. GERRIE ◽  
WILLIAM SINGER ◽  
EVA HORVATH ◽  
KALMAN KOVACS ◽  
...  
Keyword(s):  
Null Cell ◽  
Human Pituitary ◽  
Gonadotropin Secretion ◽  
Null Cell Adenomas
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