scholarly journals Line Scan Raman Microspectroscopy for Label-Free Diagnosis of Human Pituitary Biopsies

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3577 ◽  
Author(s):  
Daniela Bovenkamp ◽  
Alexander Micko ◽  
Jeremias Püls ◽  
Fabian Placzek ◽  
Romana Höftberger ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Pituitary Adenomas ◽  
Nearest Neighbor ◽  
Principal Component ◽  
Raman Microspectroscopy ◽  
Label Free ◽  
K Nearest Neighbor ◽  
Null Cell ◽  
Human Pituitary ◽  
Line Scan

Pituitary adenomas are neoplasia of the anterior pituitary gland and can be subdivided into hormone-producing tumors (lactotroph, corticotroph, gonadotroph, somatotroph, thyreotroph or plurihormonal) and hormone-inactive tumors (silent or null cell adenomas) based on their hormonal status. We therefore developed a line scan Raman microspectroscopy (LSRM) system to detect, discriminate and hyperspectrally visualize pituitary gland from pituitary adenomas based on molecular differences. By applying principal component analysis followed by a k-nearest neighbor algorithm, specific hormone states were identified and a clear discrimination between pituitary gland and various adenoma subtypes was achieved. The classifier yielded an accuracy of 95% for gland tissue and 84–99% for adenoma subtypes. With an overall accuracy of 92%, our LSRM system has proven its potential to differentiate pituitary gland from pituitary adenomas. LSRM images based on the presence of specific Raman bands were created, and such images provided additional insight into the spatial distribution of particular molecular compounds. Pathological states could be molecularly differentiated and characterized with texture analysis evaluating Grey Level Cooccurrence Matrices for each LSRM image, as well as correlation coefficients between LSRM images.

scholarly journals Morpho-Molecular Metabolic Analysis and Classification of Human Pituitary Gland and Adenoma Biopsies Based on Multimodal Optical Imaging

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3234
Author(s):  
Gabriel Giardina ◽  
Alexander Micko ◽  
Daniela Bovenkamp ◽  
Arno Krause ◽  
Fabian Placzek ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Optical Imaging ◽  
Pituitary Adenomas ◽  
Multiphoton Microscopy ◽  
Chemical Properties ◽  
Tissue Imaging ◽  
Label Free ◽  
Null Cell ◽  
Human Pituitary ◽  
Metabolic Analysis

Pituitary adenomas count among the most common intracranial tumors. During pituitary oncogenesis structural, textural, metabolic and molecular changes occur which can be revealed with our integrated ultrahigh-resolution multimodal imaging approach including optical coherence tomography (OCT), multiphoton microscopy (MPM) and line scan Raman microspectroscopy (LSRM) on an unprecedented cellular level in a label-free manner. We investigated 5 pituitary gland and 25 adenoma biopsies, including lactotroph, null cell, gonadotroph, somatotroph and mammosomatotroph as well as corticotroph. First-level binary classification for discrimination of pituitary gland and adenomas was performed by feature extraction via radiomic analysis on OCT and MPM images and achieved an accuracy of 88%. Second-level multi-class classification was performed based on molecular analysis of the specimen via LSRM to discriminate pituitary adenomas subtypes with accuracies of up to 99%. Chemical compounds such as lipids, proteins, collagen, DNA and carotenoids and their relation could be identified as relevant biomarkers, and their spatial distribution visualized to provide deeper insight into the chemical properties of pituitary adenomas. Thereby, the aim of the current work was to assess a unique label-free and non-invasive multimodal optical imaging platform for pituitary tissue imaging and to perform a multiparametric morpho-molecular metabolic analysis and classification.

Two-parameter KNN algorithm and its application in recognition of brand rice

2021 ◽  
pp. 1-7
Author(s):  
Zhu Siyu ◽  
He Chongnan ◽  
Song Mingjuan ◽  
Li Linna
Keyword(s):  
Near Infrared ◽  
Nearest Neighbor ◽  
Recognition Accuracy ◽  
Principal Component ◽  
Kernel Principal Component Analysis ◽  
K Nearest Neighbor ◽  
Fisher Discriminant Analysis ◽  
Pattern Class ◽  
Fisher Discriminant ◽  
Two Parameter

In response to the frequent counterfeiting of Wuchang rice in the market, an effective method to identify brand rice is proposed. Taking the near-infrared spectroscopy data of a total of 373 grains of rice from the four origins (Wuchang, Shangzhi, Yanshou, and Fangzheng) as the observations, kernel principal component analysis(KPCA) was employed to reduce the dimensionality, and Fisher discriminant analysis(FDA) and k-nearest neighbor algorithm (KNN) were used to identify brand rice respectively. The effects of the two recognition methods are very good, and that of KNN is relatively better. Howerver the shortcomings of KNN are obvious. For instance, it has only one test dimension and its test of samples is not delicate enough. In order to further improve the recognition accuracy, fuzzy k-nearest neighbor set is defined and fuzzy probability theory is employed to get a new recognition method –Two-Parameter KNN discrimination method. Compared with KNN algorithm, this method increases the examination dimension. It not only examines the proportion of the number of samples in each pattern class in the k-nearest neighbor set, but also examines the degree of similarity between the center of each pattern class and the sample to be identified. Therefore, the recognition process is more delicate and the recognition accuracy is higher. In the identification of brand rice, the discriminant accuracy of Two-Parameter KNN algorithm is significantly higher than that of FDA and that of KNN algorithm.

scholarly journals Feature and Decision Level Fusion in Children Multimodal Biometrics

2020 ◽  
Vol 8 (5) ◽  
pp. 2522-2527
Keyword(s):  
Nearest Neighbor ◽  
Principal Component ◽  
Identification Accuracy ◽  
Support Vector ◽  
Biometric System ◽  
K Nearest Neighbor ◽  
Decision Level ◽  
Multimodal Biometric System ◽  
Decision Level Fusion ◽  
Level Fusion

In this paper, we design method for recognition of fingerprint and IRIS using feature level fusion and decision level fusion in Children multimodal biometric system. Initially, Histogram of Gradients (HOG), Gabour and Maximum filter response are extracted from both the domains of fingerprint and IRIS and considered for identification accuracy. The combination of feature vector of all the possible features is recommended by biometrics traits of fusion. For fusion vector the Principal Component Analysis (PCA) is used to select features. The reduced features are fed into fusion classifier of K-Nearest Neighbor (KNN), Support Vector Machine (SVM), Navie Bayes(NB). For children multimodal biometric system the suitable combination of features and fusion classifiers is identified. The experimentation conducted on children’s fingerprint and IRIS database and results reveal that fusion combination outperforms individual. In addition the proposed model advances the unimodal biometrics system.

scholarly journals Analisis Metode Pengenalan Wajah Two Dimensial Principal Component Analysis (2DPCA) dan Kernel Fisher Discriminant Analysis Menggunakan Klasifikasi KNN (K- Nearest Neighbor)

2020 ◽  
Vol 2 (2) ◽  
pp. 29-38
Author(s):  
Abdur Rohman Harits Martawireja ◽  
Hilman Mujahid Purnama ◽  
Atika Nur Rahmawati
Keyword(s):  
Principal Component Analysis ◽  
Discriminant Analysis ◽  
Cross Validation ◽  
Nearest Neighbor ◽  
Principal Component ◽  
Component Analysis ◽  
K Nearest Neighbor ◽  
Fisher Discriminant Analysis ◽  
Fisher Discriminant ◽  
Kernel Fisher Discriminant Analysis

Pengenalan wajah manusia (face recognition) merupakan salah satu bidang penelitian yang penting dan belakangan ini banyak aplikasi yang menerapkannya, baik di bidang komersil ataupun di bidang penegakan hukum. Pengenalan wajah merupakan sebuah sistem yang berfungsikan untuk mengidentifikasi berdasarkan ciri-ciri dari wajah seseorang berbasis biometrik yang memiliki keakuratan tinggi. Pengenalan wajah dapat diterapkan pada sistem keamanan. Banyak metode yang dapat digunakan dalam aplikasi pengenalan wajah untuk keamanan sistem, namun pada artikel ini akan membahas tentang dua metode yaitu Two Dimensial Principal Component Analysis dan Kernel Fisher Discriminant Analysis dengan metode klasifikasi menggunakan K-Nearest Neigbor. Kedua metode ini diuji menggunakan metode cross validation. Hasil dari penelitian terdahulu terbukti bahwa sistem pengenalan wajah metode Two Dimensial Principal Component Analysis dengan 5-folds cross validation menghasilkan akurasi sebesar 88,73%, sedangkan dengan 2-folds validation akurasi yang dihasilkan sebesar 89,25%. Dan pengujian metode Kernel Fisher Discriminant dengan 2-folds cross validation menghasilkan akurasi rata rata sebesar 83,10%.

scholarly journals A Study on the Psychological Analysis System Using Machine Learning

2018 ◽  
Vol 7 (3.33) ◽  
pp. 128
Author(s):  
Ki Young Lee ◽  
Kyu Ho Kim ◽  
Jeong Jin Kang ◽  
Sung Jai Choi ◽  
Yong Soon Im ◽  
...  
Keyword(s):  
Machine Learning ◽  
Nearest Neighbor ◽  
Smart Phone ◽  
Principal Component ◽  
Digital Data ◽  
Expression Recognition ◽  
K Nearest Neighbor ◽  
Linear Discriminant ◽  
Psychological Analysis ◽  
Analysis System

Real-time facial expression recognition and analysis technology is recently drawing attention in areas of computer vision, computer graphics, and HCI. Recognition of user’s emotion on the basis of video and voice is drawing particular interest. The technology may help managers of households or hospitals. In the present study, video and voice were converted into digital data through MATLAB by using PCA(Principal Component Analysis), LDA(Linear Discriminant Analysis), KNN(K Nearest Neighbor) algorithms to analyze emotions through machine learning. The manager of the psychological analysis counseling system may understand a user’s emotion in an smart phone environment. This system of the present study may help the manager to have a smooth conversation or develop a smooth relationship with a user on the basis of the provided psychological analysis results. 

scholarly journals Discrimination of Chinese Liquors Based on Electronic Nose and Fuzzy Discriminant Principal Component Analysis

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 38 ◽  
Author(s):  
Xiaohong Wu ◽  
Jin Zhu ◽  
Bin Wu ◽  
Chao Zhao ◽  
Jun Sun ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Feature Extraction ◽  
Electronic Nose ◽  
Nearest Neighbor ◽  
Principal Component ◽  
Component Analysis ◽  
K Nearest Neighbor ◽  
Knn Classifier ◽  
Extraction Algorithm ◽  
Leave One Out

The detection of liquor quality is an important process in the liquor industry, and the quality of Chinese liquors is partly determined by the aromas of the liquors. The electronic nose (e-nose) refers to an artificial olfactory technology. The e-nose system can quickly detect different types of Chinese liquors according to their aromas. In this study, an e-nose system was designed to identify six types of Chinese liquors, and a novel feature extraction algorithm, called fuzzy discriminant principal component analysis (FDPCA), was developed for feature extraction from e-nose signals by combining discriminant principal component analysis (DPCA) and fuzzy set theory. In addition, principal component analysis (PCA), DPCA, K-nearest neighbor (KNN) classifier, leave-one-out (LOO) strategy and k-fold cross-validation (k = 5, 10, 20, 25) were employed in the e-nose system. The maximum classification accuracy of feature extraction for Chinese liquors was 98.378% using FDPCA, showing this algorithm to be extremely effective. The experimental results indicate that an e-nose system coupled with FDPCA is a feasible method for classifying Chinese liquors.

A Robust Physical Exercise Recognition System Using Machine Learning Approach

2020 ◽  
Vol 1 (1) ◽  
pp. 17-21
Author(s):  
Steve Oscar ◽  
◽  
Mohammed Nazim Uddin ◽  
Keyword(s):  
Physical Exercise ◽  
Nearest Neighbor ◽  
Principal Component ◽  
Recognition System ◽  
Accelerometer Data ◽  
K Nearest Neighbor ◽  
Machine Learning Approach ◽  
Principal Component Analysis Algorithm ◽  
K Nearest Neighbor Algorithm

Modern life is becoming more linked to our devices, and work is being done in a more regulated way. As life became more complicated, it is becoming challenging to keep track of human health and fitness, leading to unexpected illnesses and diseases. Moreover, a lack of activity monitoring and corresponding reminders is preventing the adoption of a healthier lifestyle. This research provides a practical approach for identifying Human Activity by using accelerometer data obtained from wearable devices. The model automatically finds patterns among 33 different physical exercises such as running, rowing, cycling, jogging, etc. and correctly identifies them. The principal component analysis algorithm was used on the statistical features to make the system more robust. Classification of the physical exercise was performed on the reduced features using WEKA. The overall accuracy of 85.51% was obtained using the 10-Fold Cross-Validation method and K nearest Neighbor Algorithm while 84% accuracy for Random Forest. The accuracy obtained was better than previous models and could improve recognition systems in monitoring user activity more precisely.

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