Plant chromosome analysis with the giemsa C-banding technique

1983 ◽  
Vol 1 (4) ◽  
pp. 22-26
Author(s):  
Alan R. Gould ◽  
Sheryl A. Mayer
Keyword(s):  
Chromosome Analysis ◽  
Banding Technique ◽  
Plant Chromosome ◽  
C Banding

Pulmonary Alveolar Proteinosis as a Terminal Complication in a Case of Myelodysplastic Syndrome with Idic(20q-).

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4864-4864
Author(s):  
Yongquan Xue ◽  
Yongsheng Han ◽  
Tianyu Li ◽  
Suning Chen ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Pulmonary Alveolar Proteinosis ◽  
Conflicts Of Interest ◽  
Periodic Acid ◽  
Chromosome Analysis ◽  
Banding Technique ◽  
Chromosomal Marker ◽  
Fish Analysis ◽  
Periodic Acid Schiff ◽  
Computed Tomographic ◽  
Alveolar Proteinosis

Abstract Abstract 4864 Secondary pulmonary alveolar proteinosis (PAP) is a rare lung disease which was reported in 13 cases of myelodysplastic syndromes (MDS). A dicentric isochromosome of deleted chromosome 20q i.e., idic(20q-) is a newly recognized rare but recurrent cytogenetic anomaly which has been described in thirty cases of MDS. Recently we encounted an interesting MDS patient with idic(20q-) and secondary PAP. At presentation she was a forty-year-old women manifesting pancytopenia and dysplasia involving two cell lineages which were compatible with refractory cytopenia with multilineage desplasia. Chromosome analysis using R-banding technique showed a karyotype of 46,XX,-20,+a small metacentric chromosomal marker. FISH analysis proved this marker to be a dicentric isochromosome of a deleted 20q. Three yaeas after presentation her disease was complicated by secondary PAP which was confirmed by chest computed tomographic scan and the thoracoscopic lung biopsy which revealed the characteristic periodic acid Schiff stain-positive materials filling the alveoli. After prednisolone and intibiotics freatment, her symptoms had temporary improvement. At last she died of respiratory failure with a survival of 45 months. As we know, no MDS patients with idic(20q-) and secondary PAP have been reported in the literature. Thus, this patient is not only the thirty-first MDS case with idic(20q-), but also the first MDS case with idic(20q-) and secondary PAP. Disclosures No relevant conflicts of interest to declare.

Standard Giemsa C-banded karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion–translocation heterozygote

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1262-1270 ◽  
Author(s):  
Jun-Zhi Wei ◽  
William F. Campbell ◽  
Richard R.-C. Wang
Keyword(s):  
Banding Pattern ◽  
Geographic Area ◽  
B Chromosome ◽  
Distal Segment ◽  
Banding Technique ◽  
Translocation Heterozygote ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Geographical Regions ◽  
Russian Wildrye

Ten accessions of Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), collected from different geographical regions were analyzed using the C-banding technique. C-banding pattern polymorphisms were observed at all levels, i.e., within homologous chromosome pairs of the same plant, among different individuals within accessions, between different accessions of the same geographic area, and among accessions of different origins. The seven homologous groups varied in the level of C-banding pattern polymorphism; chromosomes A, B, E, and F were more variable than chromosomes C, D, and G. The polymorphisms did not hamper chromosome identification in Ps. juncea, because each chromosome pair of the Ns genome had a different basic C-banding pattern and karyotypic character. A standard C-banded karyotype of Ps. juncea is proposed based on the overall karyotypes and C-bands in the 10 accessions. The C-bands on the Ns-genome chromosomes were designated according to the rules of nomenclature used in wheat. A deletion–translocation heterozygote of Russian wildrye was identified based on the karyotype and C-banding patterns established. The chromosome F pair consisted of a chromosome having the distal segment in the long arm deleted and a translocated chromosome having the distal segment of long arm replaced by the distal segment of the long arm of chromosome E. The chromosome E pair had a normal chromosome E and a translocated chromosome having the short arm and the proximal segment of the long arm of chromosome E and the distal segment of the long arm of chromosome F.Key words: Psathyrostachys juncea, karyotype, Giemsa C-banding, polymorphism, B chromosome.

Analysis of intraspecific diversity of cultivated emmer Triticum dicoccum (Schrank.) schuebl using C-banding technique

2007 ◽  
Vol 43 (11) ◽  
pp. 1271-1285 ◽  
Author(s):  
O. S. Dedkova ◽  
E. D. Badaeva ◽  
O. P. Mitrofanova ◽  
E. N. Bilinskaya ◽  
V. A. Pukhalskiy
Keyword(s):  
Intraspecific Diversity ◽  
Banding Technique ◽  
Triticum Dicoccum ◽  
C Banding

Rye–wheat hybrids: the production of wheat chromosome additions to rye

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 840-844 ◽  
Author(s):  
M. Baum
Keyword(s):  
Wheat Chromosome ◽  
Banding Technique ◽  
Addition Lines ◽  
C Banding ◽  
Good Potential ◽  
Wheat Hybrids

To produce rye–wheat addition lines, 21-chromosome rye–wheat hybrids were produced by crossing tetraploid triticale with diploid rye. The subsequent selfing of the hybrids lead to monosomic and double monosomic wheat additions to rye. Screening of the progeny was carried out using the C-banding technique. The wheat additions can be fertile. Wheat chromosome 6B was the chromosome most frequently added to rye. Ribosomal spacer probe pTa250.4 was used to confirm the results obtained by C-banding for the 6B wheat additions to rye. Embryos of the 21-chromosome rye–wheat hybrids showed a good potential for propagating more plantlets after they had been transferred to artificial medium.Key words: rye–wheat addition lines.

Modified Method of C Banding Using Barium Hydroxide

1975 ◽  
Vol 24 (3-4) ◽  
pp. 315-316
Author(s):  
P.K. Ghosh ◽  
Indera P. Singh
Keyword(s):  
Barium Hydroxide ◽  
Centromeric Heterochromatin ◽  
Banding Technique ◽  
C Banding ◽  
Modified Method ◽  
The Relationship

A modified centromeric heterochromatin banding technique using barium hydroxide octahydrate is described. The relationship between slide maturity and time of denaturation by barium-hydroxide is discussed.

scholarly journals Karyotyping the Arabian Camel (Camelus dromedarius), Using C-Banding Technique.

CYTOLOGIA ◽  
1992 ◽  
Vol 57 (3) ◽  
pp. 383-388 ◽  
Author(s):  
Mohammed A. Samman ◽  
Abdulaziz A. Al-Saleh ◽  
Kart Sheth
Keyword(s):  
Camelus Dromedarius ◽  
Banding Technique ◽  
C Banding ◽  
Arabian Camel

Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum)

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 830-839 ◽  
Author(s):  
B. S. Gill ◽  
B. Friebe ◽  
T. R. Endo
Keyword(s):  
Triticum Aestivum ◽  
Wheat Chromosome ◽  
Chromosome Analysis ◽  
Triticum Aestivum L ◽  
Nomenclature System ◽  
C Banding ◽  
Structural Aberrations ◽  
Individual Bands ◽  
Standard Karyotype ◽  
Band Number

A standard karyotype based on N-banding, C-banding, and modified C-banding has been constructed for Triticum aestivum L. 'Chinese Spring'. An idiogram and a nomenclature system have been developed for the description of individual bands. Nomenclatural rules have been proposed for the description of chromosomal structural aberrations and polymorphic bands in other wheat cultivars. As a rule each short arm (S) and a long arm (L) consists of a series of dark bands (C-bands) and light bands (mainly euchromatic) and by definition there are no interbands. In some cases, each arm has been subdivided into two or more regions. The description of a band requires designation of a chromosome number, arm (S or L), region, and band. The region number is separated from the band number by a decimal point. Except for arms 1AS, 3AL, 4AS, and 6AS, all wheat chromosome arms have one or more intercalary C-bands and are divisible into three or more bands. It is hoped that the proposed karyotype and nomenclature system will be widely adopted and lay the foundation of definitive chromosome analysis in wheat.Key words: C-banding, N-banding, common wheat, heterochromatin, idiogram.

C-banding polymorphisms in several accessions of Triticum tauschii (Aegilops squarrosa)

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 192-199 ◽  
Author(s):  
B. Friebe ◽  
Y. Mukai ◽  
B. S. Gill
Keyword(s):  
In Situ Hybridization ◽  
Banding Pattern ◽  
Chromosome Analysis ◽  
Polymorphic Variation ◽  
D Genome ◽  
Minor Variation ◽  
Triticum Tauschii ◽  
C Banding ◽  
Aegilops Squarrosa

A generalized C-banded karyotype of Triticum tauschii (Aegilops squarrosa) was established based on chromosome analysis of 15 accessions of diverse origin, including the two varieties eusquarrosa (with the former varieties typica, anathera, and meyeri) and strangulata. The C-banding pattern of T. tauschii chromosomes was similar to the D-genome chromosomes of cultivated bread wheat, T. aestivum, thus permitting their unequivocal identification and homoeologous chromosome designations. Whereas only minor variation in C-banding pattern was observed within the accessions, a large amount of polymorphic variation was found among the different accessions. However, this polymorphic variation did not prevent chromosome identification in these lines. One accession (TA 2462) was found to be homozygous for a reciprocal translocation involving the complete arms of chromosomes 1D and 7D. In situ hybridization using the D-genome specific probe pAS1 confirmed the presence of T1DS-7DL and T7DS.1DL translocation in the accession TA 2462.Key words: Triticum tauschii, C-banding, in situ hybridization.

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