Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation

1990 ◽  
Vol 26 (7) ◽  
pp. 709-712 ◽  
Author(s):  
Alain Limat ◽  
Thomas Hunziker ◽  
Colette Boillat ◽  
Friedrich Noser ◽  
Ulrich Wiesmann
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

Long-term fate of silica nanoparticles interacting with human dermal fibroblasts

Biomaterials ◽  
2012 ◽  
Vol 33 (17) ◽  
pp. 4431-4442 ◽  
Author(s):  
Sandrine Quignard ◽  
Gervaise Mosser ◽  
Michel Boissière ◽  
Thibaud Coradin
Keyword(s):  
Silica Nanoparticles ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

Artificial extracellular matrices support cell growth and matrix synthesis of human dermal fibroblasts in macroporous 3D scaffolds

2015 ◽  
Vol 11 (5) ◽  
pp. 1390-1402 ◽  
Author(s):  
Anja van der Smissen ◽  
Peter-Georg Hoffmeister ◽  
Nadja Friedrich ◽  
Akira Watarai ◽  
Michael C. Hacker ◽  
...  
Keyword(s):  
Cell Growth ◽  
Dermal Fibroblasts ◽  
Extracellular Matrices ◽  
Human Dermal Fibroblasts ◽  
Matrix Synthesis ◽  
3D Scaffolds ◽  
Support Cell

scholarly journals Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts

2011 ◽  
Vol 32 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Dong-Wook Han ◽  
Mi Hee Lee ◽  
Hak Hee Kim ◽  
Suong-Hyu Hyon ◽  
Jong-Chul Park
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts

scholarly journals Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

2010 ◽  
Vol 2010 ◽  
pp. 1-5
Author(s):  
Zhi-Gang Xue ◽  
Zhan-Ping Shi ◽  
Juan Dong ◽  
Ting-Ting Liao ◽  
Yan-Peng Wang ◽  
...  
Keyword(s):  
X Chromosome ◽  
Normal Karyotype ◽  
Biological Properties ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
X Chromosomes ◽  
High Passage ◽  
Primary Fibroblasts ◽  
Induced Pluripotent

Human primary fibroblasts are a popular type of somatic cells for the production of induced pluripotent stem (iPS) cells. Here we characterized biological properties of primary fibroblasts in terms of cell-growth rate, cytogenetic stability, and the number of inactive X chromosomes during long-term passaging. We produced eight lines of female human dermal fibroblasts (HDFs) and found normal karyotype and expected pattern of X chromosome inactivation (XCI) at low passages (Passage P1-5). However, four out of the eight HDF lines at high passage numbers (≥P10) exhibited duplicated hallmarks of inactive X chromosome including two punctuate signals of histone H3 lysine 27 trimethylation (H3K27me3) and X inactive-specific transcript (XIST) RNA signals in approximately 8.5–18.5% of the cells. Our data suggest that the copy number of inactive X chromosomes in a subset of female HDF is increased by a two-fold. Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs. We conclude that female HDF cultures exhibit a higher risk of genetic anomalies such as carrying an increased number of X chromosomes including both active and inactive X chromosomes at a high passage (≥P10).

scholarly journals Involvement of 8-O-acetylharpagide for Ajuga taiwanensis mediated suppression of senescent phenotypes in human dermal fibroblasts

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wei-Hsiang Hsu ◽  
Bing-Ze Lin ◽  
Jyh-Der Leu ◽  
Pin-Ho Lo ◽  
Hsueh-Yen Yu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Herbal Medicines ◽  
Current Data ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Active Constituent ◽  
Alcohol Extract ◽  
G1 Phase Arrest ◽  
Human Care

AbstractHerbal medicines are attractive agents for human care. In this study, we found that the alcohol extract of Ajuga taiwanensis (ATE) screened from a chemical bank exhibited potent capacity for suppressing senescence associated biomarkers, including SA-β-gal and up-regulated p53 in old human dermal fibroblasts (HDFs) without induction of significant cytotoxicity up to 100 µg/ml. Concomitantly, cells re-entered the cell cycle by reducing G1 phase arrest and increasing cell growth rate. The ATE was further partitioned to obtain the sub-fractions of n-butanol (BuOH), ethyl acetate (EA) and water. The BuOH and water sub-fractions exhibited less effects on prohibition of cell growth than the EA sub-fraction. All of these sub-fractions exhibited the ability on suppressing SA-β-gal and p53 of old HDFs as low as 5–10 µg/ml. Under the activity guided fractionation and isolation, a major active constituent named AT-1 was isolated. The AT-1 was further identified as 8-O-acetylharpagide by structural analysis, and it could suppress SA-β-gal and p53 of old HDFs below 10 µM. In addition, the intracellular reactive oxygen species (ROS) levels of old HDFs were suppressed by ATE, the sub-fractions of BuOH and water, and AT-1. However, the EA sub-fraction showed little ability on suppression of ROS. Furthermore, we performed an in vivo study using aging mice to be fed with ATE and the sub-fractions followed by immunohistochemical (IHC) staining. The expression of p53 and SA-β-gal was significantly reduced in several tissue sections, including skin, liver, kidney, and spleen. Taken together, current data demonstrated that A. taiwanensis could suppress cellular senescence in HDFs, and might be used for health care.

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