The effect of a long-term cyclic strain on human dermal fibroblasts cultured in a bioreactor on chitosan-based scaffolds for the development of tissue engineered artificial dermis

2007 ◽  
Vol 15 (4) ◽  
pp. 370-378 ◽  
Author(s):  
Sae-Hwan Lim ◽  
Youngsook Son ◽  
Chun-Ho Kim ◽  
Heungsoo Shin ◽  
Jong Il Kim
Keyword(s):  
Cyclic Strain ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Artificial Dermis

Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation

1990 ◽  
Vol 26 (7) ◽  
pp. 709-712 ◽  
Author(s):  
Alain Limat ◽  
Thomas Hunziker ◽  
Colette Boillat ◽  
Friedrich Noser ◽  
Ulrich Wiesmann
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

Long-term fate of silica nanoparticles interacting with human dermal fibroblasts

Biomaterials ◽  
2012 ◽  
Vol 33 (17) ◽  
pp. 4431-4442 ◽  
Author(s):  
Sandrine Quignard ◽  
Gervaise Mosser ◽  
Michel Boissière ◽  
Thibaud Coradin
Keyword(s):  
Silica Nanoparticles ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture

2010 ◽  
Vol 2010 ◽  
pp. 1-5
Author(s):  
Zhi-Gang Xue ◽  
Zhan-Ping Shi ◽  
Juan Dong ◽  
Ting-Ting Liao ◽  
Yan-Peng Wang ◽  
...  
Keyword(s):  
X Chromosome ◽  
Normal Karyotype ◽  
Biological Properties ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
X Chromosomes ◽  
High Passage ◽  
Primary Fibroblasts ◽  
Induced Pluripotent

Human primary fibroblasts are a popular type of somatic cells for the production of induced pluripotent stem (iPS) cells. Here we characterized biological properties of primary fibroblasts in terms of cell-growth rate, cytogenetic stability, and the number of inactive X chromosomes during long-term passaging. We produced eight lines of female human dermal fibroblasts (HDFs) and found normal karyotype and expected pattern of X chromosome inactivation (XCI) at low passages (Passage P1-5). However, four out of the eight HDF lines at high passage numbers (≥P10) exhibited duplicated hallmarks of inactive X chromosome including two punctuate signals of histone H3 lysine 27 trimethylation (H3K27me3) and X inactive-specific transcript (XIST) RNA signals in approximately 8.5–18.5% of the cells. Our data suggest that the copy number of inactive X chromosomes in a subset of female HDF is increased by a two-fold. Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs. We conclude that female HDF cultures exhibit a higher risk of genetic anomalies such as carrying an increased number of X chromosomes including both active and inactive X chromosomes at a high passage (≥P10).

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