Artificial extracellular matrices support cell growth and matrix synthesis of human dermal fibroblasts in macroporous 3D scaffolds

2015 ◽  
Vol 11 (5) ◽  
pp. 1390-1402 ◽  
Author(s):  
Anja van der Smissen ◽  
Peter-Georg Hoffmeister ◽  
Nadja Friedrich ◽  
Akira Watarai ◽  
Michael C. Hacker ◽  
...  
Keyword(s):  
Cell Growth ◽  
Dermal Fibroblasts ◽  
Extracellular Matrices ◽  
Human Dermal Fibroblasts ◽  
Matrix Synthesis ◽  
3D Scaffolds ◽  
Support Cell

Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation

1990 ◽  
Vol 26 (7) ◽  
pp. 709-712 ◽  
Author(s):  
Alain Limat ◽  
Thomas Hunziker ◽  
Colette Boillat ◽  
Friedrich Noser ◽  
Ulrich Wiesmann
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

Growth promoting substrates for human dermal fibroblasts provided by artificial extracellular matrices composed of collagen I and sulfated glycosaminoglycans

Biomaterials ◽  
2011 ◽  
Vol 32 (34) ◽  
pp. 8938-8946 ◽  
Author(s):  
Anja van der Smissen ◽  
Vera Hintze ◽  
Dieter Scharnweber ◽  
Stephanie Moeller ◽  
Matthias Schnabelrauch ◽  
...  
Keyword(s):  
Dermal Fibroblasts ◽  
Collagen I ◽  
Extracellular Matrices ◽  
Human Dermal Fibroblasts ◽  
Growth Promoting

scholarly journals Efficient Decellularization by Application of Moderate High Hydrostatic Pressure with Supercooling Pretreatment

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1486
Author(s):  
Daiki Zemmyo ◽  
Masashi Yamamoto ◽  
Shogo Miyata
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Biological Tissues ◽  
Dermal Fibroblasts ◽  
Extracellular Matrices ◽  
Human Dermal Fibroblasts ◽  
Collagen Gels ◽  
Collagen Network ◽  
Chemical Methods ◽  
Almost All

Decellularized tissues are considered superior scaffolds for cell cultures, preserving the microstructure of native tissues and delivering many kinds of cytokines. High hydrostatic pressure (HHP) treatment could remove cells physically from biological tissues rather than chemical methods. However, there are some risks of inducing destruction or denaturation of extracellular matrices (ECMs) at an ultrahigh level of HHP. Therefore, efficient decellularization using moderate HHP is required to remove almost all cells simultaneously to suppress tissue damage. In this study, we proposed a novel decellularization method using a moderate HHP with supercooling pretreatment. To validate the decellularization method, a supercooling device was developed to incubate human dermal fibroblasts or collagen gels in a supercooled state. The cell suspension and collagen gels were subjected to 100, 150, and 200 MPa of HHP after supercooling pretreatment, respectively. After applying HHP, the viability and morphology of the cells and the collagen network structure of the gels were evaluated. The viability of cells decreased dramatically after HHP application with supercooling pretreatment, whereas the microstructures of collagen gels were preserved and cell adhesivity was retained after HHP application. In conclusion, it was revealed that supercooling pretreatment promoted the denaturation of the cell membrane to improve the efficacy of decellularization using static application of moderate HHP. Furthermore, it was demonstrated that the HHP with supercooling pretreatment did not degenerate and damage the microstructure in collagen gels.

scholarly journals Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts

2011 ◽  
Vol 32 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Dong-Wook Han ◽  
Mi Hee Lee ◽  
Hak Hee Kim ◽  
Suong-Hyu Hyon ◽  
Jong-Chul Park
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts

scholarly journals Involvement of 8-O-acetylharpagide for Ajuga taiwanensis mediated suppression of senescent phenotypes in human dermal fibroblasts

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wei-Hsiang Hsu ◽  
Bing-Ze Lin ◽  
Jyh-Der Leu ◽  
Pin-Ho Lo ◽  
Hsueh-Yen Yu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Herbal Medicines ◽  
Current Data ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Active Constituent ◽  
Alcohol Extract ◽  
G1 Phase Arrest ◽  
Human Care

AbstractHerbal medicines are attractive agents for human care. In this study, we found that the alcohol extract of Ajuga taiwanensis (ATE) screened from a chemical bank exhibited potent capacity for suppressing senescence associated biomarkers, including SA-β-gal and up-regulated p53 in old human dermal fibroblasts (HDFs) without induction of significant cytotoxicity up to 100 µg/ml. Concomitantly, cells re-entered the cell cycle by reducing G1 phase arrest and increasing cell growth rate. The ATE was further partitioned to obtain the sub-fractions of n-butanol (BuOH), ethyl acetate (EA) and water. The BuOH and water sub-fractions exhibited less effects on prohibition of cell growth than the EA sub-fraction. All of these sub-fractions exhibited the ability on suppressing SA-β-gal and p53 of old HDFs as low as 5–10 µg/ml. Under the activity guided fractionation and isolation, a major active constituent named AT-1 was isolated. The AT-1 was further identified as 8-O-acetylharpagide by structural analysis, and it could suppress SA-β-gal and p53 of old HDFs below 10 µM. In addition, the intracellular reactive oxygen species (ROS) levels of old HDFs were suppressed by ATE, the sub-fractions of BuOH and water, and AT-1. However, the EA sub-fraction showed little ability on suppression of ROS. Furthermore, we performed an in vivo study using aging mice to be fed with ATE and the sub-fractions followed by immunohistochemical (IHC) staining. The expression of p53 and SA-β-gal was significantly reduced in several tissue sections, including skin, liver, kidney, and spleen. Taken together, current data demonstrated that A. taiwanensis could suppress cellular senescence in HDFs, and might be used for health care.

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