scholarly journals Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts

2011 ◽  
Vol 32 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Dong-Wook Han ◽  
Mi Hee Lee ◽  
Hak Hee Kim ◽  
Suong-Hyu Hyon ◽  
Jong-Chul Park
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts

scholarly journals Inhibition of 3-phosphoinositide–dependent protein kinase 1 (PDK1) can revert cellular senescence in human dermal fibroblasts

2020 ◽  
Vol 117 (49) ◽  
pp. 31535-31546
Author(s):  
Sugyun An ◽  
Si-Young Cho ◽  
Junsoo Kang ◽  
Soobeom Lee ◽  
Hyung-Su Kim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Cellular Senescence ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Quiescent State ◽  
Human Dermal Fibroblasts ◽  
Dependent Protein Kinase ◽  
Age Related ◽  
Dependent Protein

Cellular senescence is defined as a stable, persistent arrest of cell proliferation. Here, we examine whether senescent cells can lose senescence hallmarks and reenter a reversible state of cell-cycle arrest (quiescence). We constructed a molecular regulatory network of cellular senescence based on previous experimental evidence. To infer the regulatory logic of the network, we performed phosphoprotein array experiments with normal human dermal fibroblasts and used the data to optimize the regulatory relationships between molecules with an evolutionary algorithm. From ensemble analysis of network models, we identified 3-phosphoinositide–dependent protein kinase 1 (PDK1) as a promising target for inhibitors to convert the senescent state to the quiescent state. We showed that inhibition of PDK1 in senescent human dermal fibroblasts eradicates senescence hallmarks and restores entry into the cell cycle by suppressing both nuclear factor κB and mTOR signaling, resulting in restored skin regeneration capacity. Our findings provide insight into a potential therapeutic strategy to treat age-related diseases associated with the accumulation of senescent cells.

scholarly journals Acer tataricum subsp. ginnala Inhibits Skin Photoaging via Regulating MAPK/AP-1, NF-κB, and TGFβ/Smad Signaling in UVB-Irradiated Human Dermal Fibroblasts

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 662
Author(s):  
Yu-Jung Jin ◽  
Yura Ji ◽  
Young-Pyo Jang ◽  
Se-Young Choung
Keyword(s):  
Collagen Synthesis ◽  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts ◽  
Smad Pathway ◽  
Type I Procollagen ◽  
Skin Photoaging ◽  
Pro Inflammatory Cytokines

Skin, the organ protecting the human body from external factors, maintains structural and tensile strength by containing many collagen fibrils, particularly type I procollagen. However, oxidative stress by ultraviolet (UV) exposure causes skin photoaging by activating collagen degradation and inhibiting collagen synthesis. Acer tataricum subsp. ginnala extract (AGE) is a herbal medicine with anti-inflammatory and anti-oxidative effects, but there is no report on the protective effect against skin photoaging. Therefore, we conducted research concentrating on the anti-photoaging effect of Acer tataricum subsp. ginnala (AG) in UVB (20 mJ/cm2)-irradiated human dermal fibroblasts (HDF). Then, various concentrations (7.5, 15, 30 µg/mL) of AGE were treated in HDF for 24 h following UVB irradiation. After we performed AGE treatment, the matrix metalloproteinase1 (MMP1) expression was downregulated, and the type I procollagen level was recovered. Then, we investigated the mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) and nuclear factor-κB (NF-κB) pathway, which induce collagen breakdown by promoting the MMP1 level and pro-inflammatory cytokines. The results indicated that AGE downregulates the expression of the MAPK/AP-1 pathway, leading to MMP1 reduction. AGE inhibits nuclear translocation of NF-κB and inhibitor of nuclear factor-κB (IκB) degradation. Therefore, it downregulates the expression of MMP1 and pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 increased by UVB. Besides, the TGFβ/Smad pathway, which is mainly responsible for the collagen synthesis in the skin, was also analyzed. AGE decreases the expression of Smad7 and increases TGFβRII expression and Smad3 phosphorylation. This means that AGE stimulates the TGFβ/Smad pathway that plays a critical role in promoting collagen synthesis. Thus, this study suggests that AGE can be a functional material with anti-photoaging properties.

scholarly journals Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB

2012 ◽  
Vol 31 (2) ◽  
pp. 477-483 ◽  
Author(s):  
BO-MI HWANG ◽  
EUN-MI NOH ◽  
JONG-SUK KIM ◽  
JEONG-MI KIM ◽  
JIN-KI HWANG ◽  
...  
Keyword(s):  
Nuclear Factor Κb ◽  
Dermal Fibroblasts ◽  
Nuclear Factor ◽  
Human Dermal Fibroblasts

Postmitotic human dermal fibroblasts preserve intact feeder properties for epithelial cell growth after long-term cryopreservation

1990 ◽  
Vol 26 (7) ◽  
pp. 709-712 ◽  
Author(s):  
Alain Limat ◽  
Thomas Hunziker ◽  
Colette Boillat ◽  
Friedrich Noser ◽  
Ulrich Wiesmann
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

LncRNA RP11-670E13.6 Regulates Cell Cycle Progression in UVB Damaged Human Dermal Fibroblasts

2018 ◽  
Vol 94 (3) ◽  
pp. 589-597 ◽  
Author(s):  
Mengna Li ◽  
Li Li ◽  
Xiaofeng Zhang ◽  
Yan Yan ◽  
Baoxi Wang
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Cycle Progression
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