Highly sensitive analytical method for aluminum movement in soybean root through lumogallion staining

1997 ◽  
Vol 110 (3) ◽  
pp. 305-309 ◽  
Author(s):  
Tatsuhiko Kataoka ◽  
Maiko Mori ◽  
Tomoko M. Nakanishi ◽  
Satoshi Matsumoto ◽  
Akira Uchiumi
Keyword(s):  
Analytical Method ◽  
Soybean Root ◽  
Highly Sensitive

scholarly journals Establishment of a Highly Sensitive Analytical Method For Measurement of Monoamine Neurotransmitters Using Mass Spectrometry

2021 ◽  
Author(s):  
Anri Hirai ◽  
Ryo Yamazaki ◽  
Atsushi Kobayashi ◽  
Takashi Kimura ◽  
Kei Nomiyama ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Body Weight ◽  
Analytical Method ◽  
Brain Regions ◽  
Liquid Chromatography Mass Spectrometry ◽  
Monoamine Neurotransmitters ◽  
Chemical Substances ◽  
Brain Functions ◽  
Spontaneous Movements ◽  
Highly Sensitive

Abstract Monoamine neurotransmitters (MAs), including dopamine (DA) and serotonin (5-HT), regulate brain functions such as behavior, memory, and learning. Neonicotinoids are pesticides that are being used more frequently. Neonicotinoid exposure has been observed to produce neurological symptoms, such as altered spontaneous movements and anxiety-like behaviors, which are suspected to be caused by altered MA levels. However, current neurotoxicity tests are not sufficiently sensitive to make these determinations. In this study, we performed some behavior tests and developed a sensitive and accurate analytical method using liquid chromatography-mass spectrometry (LC-MS/MS) to clarify the effects of neonicotinoid administration on MAs in the brain.We orally administered the neonicotinoid imidacloprid (0, 10, and 50 mg/kg body weight) to C57BL/6NCrSlc mice. In behavior tests, the decrease of activity was observed. The LC-MS/MS quantification of MAs in various brain regions by tetrafluoroborate salt of 2,4-diphenyl-pyranylium (DPP) derivatization, which was newly developed in this study, showed a decrease in some MA levels in the olfactory bulb and the striatum.Thus, in this study, we developed a new method for the sensitive detection of MAs by LC/MS using DPP derivatization. In addition, we showed that this sensitive MA quantification is effective in clarifying the neurotoxicity caused by chemical substances.

scholarly journals An Analytical Method Based on Electrochemical Sensor for the Assessment of Insect Infestation in Flour

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 325
Author(s):  
Li Fu ◽  
Jiangwei Zhu ◽  
Hassan Karimi-Maleh
Keyword(s):  
Uric Acid ◽  
Analytical Method ◽  
Professional Training ◽  
Field Analysis ◽  
Insect Infestation ◽  
Important Indicator ◽  
Highly Sensitive ◽  
Other Substances ◽  
Highly Sensitive Detection ◽  
Electrocatalytic Reaction

Uric acid is an important indicator of the insect infestation assessment in flour. In this work, we propose a method for uric acid detection based on voltammetry. This technique is particularly considered for the physicochemical properties of flour and contains a simple pretreatment process to rapidly achieve extraction and adsorption of uric acid in flour. To achieve specific recognition of uric acid, graphene and poly(3,4-ethylenedioxythiophene) (PEDOT) were used for the adsorption and concentration of uric acid in flour. The adsorbed mixture was immobilized on the surface of a screen-printed electrode for highly sensitive detection of the uric acid. The results showed that electrocatalytic oxidation of uric acid could be achieved after adsorption by graphene and PEDOT. This electrocatalytic reaction allows its oxidation peak to be distinguished from those of other substances that commonly possess electrochemical activity. This voltammetry-based detection method is a portable and disposable analytical method. Because it is simple to operate, requires no professional training, and is inexpensive, it is a field analysis method that can be promoted.

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2113-2121 ◽  
Author(s):  
Kenji Akama ◽  
Hiroyuki Noji
Keyword(s):  
Motion Analysis ◽  
Single Particle ◽  
Analytical Method ◽  
Particle Motion ◽  
Protein Detection ◽  
Biomarker Detection ◽  
Multiple Protein ◽  
Highly Sensitive ◽  
Simple Protocol ◽  
Single Particle Motion

Homogeneous digital immunoassay is a powerful analytical method for highly sensitive biomarker detection with a simple protocol. By using this method, we demonstrated the simultaneous multiple protein detection.

Stereological analysis of partially amorphous HREM images

1987 ◽  
Vol 45 ◽  
pp. 756-757
Author(s):  
R. H. Kaspi ◽  
D. E. Luzzi
Keyword(s):  
Quantitative Analysis ◽  
Analytical Method ◽  
Amorphous Materials ◽  
Low Dose ◽  
Intermetallic Alloy ◽  
Crystalline Region ◽  
Fringe Contrast ◽  
Stereological Analysis ◽  
The Third ◽  
Highly Sensitive

The HREM is highly sensitive to the presence of crystals in a specimen. As a results, researchers often report the presence of some fringe contrast in an otherwise amorphous image. The potential for obtaining more information than just a sense of the presence of crystal has not been addressed in detail. In the interest of allowing some amount of quantitative analysis of HREM images of partially and fully amorphous materials, we have developed an analytical method with which to quantify the directionality of an image using stereology.Figure 1 is a series of HREM images taken through the crystal - amorphous interface of an electron irradiated Cu-Ti intermetallic alloy. The first image is from an unirradiated crystalline region. The second image is from a low dose irradiated region, while the third is from a region that has been irradiated to a dose just under the necessary dosage for a total amorphous transition.

Establishment of highly sensitive analytical method for chondroitin sulfate by flow injection chemiluminescence

2019 ◽  
Vol 206 ◽  
pp. 504-510 ◽  
Author(s):  
Siying Zhang ◽  
Guanglian Zhou ◽  
Xiao Zhang ◽  
Dong Chen ◽  
Jian Liu ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Flow Injection ◽  
Analytical Method ◽  
Highly Sensitive ◽  
Flow Injection Chemiluminescence

scholarly journals UPLC-MS/MS analysis and biological activity of the potato cyst nematode hatching stimulant, solanoeclepin A, in the root exudate of Solanum spp.

Planta ◽  
2021 ◽  
Vol 254 (6) ◽  
Author(s):  
Alessandra Guerrieri ◽  
Kristýna Floková ◽  
Lieke E. Vlaar ◽  
Mario L. Schilder ◽  
Gertjan Kramer ◽  
...  
Keyword(s):  
Root Exudates ◽  
Analytical Method ◽  
Natural Variation ◽  
Root Exudate ◽  
Plant Root ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Solanaceous Species ◽  
Highly Sensitive

Abstract Main conclusion Solanoeclepin A is a hatching stimulant for potato cyst nematode in very low (pM) concentrations. We report a highly sensitive method for the analysis of SolA in plant root exudates using UHPLC-MS/MS and show that there is considerable natural variation in SolA production in Solanum spp. corresponding with their hatching inducing activity. Abstract Potato cyst nematode (PCN) is a plant root sedentary endoparasite, specialized in the infection of solanaceous species such as potato (Solanum tuberosum) and tomato (Solanum lycopersicum). Earlier reports (Mulder et al. in Hatching agent for the potato cyst nematode, Patent application No. PCT/NL92/00126, 1996; Schenk et al. in Croat Chem Acta 72:593–606, 1999) showed that solanoeclepin A (SolA), a triterpenoid metabolite that was isolated from the root exudate of potato, induces the hatching of PCN. Its low concentration in potato root exudate has hindered progress in fully understanding its hatching inducing activity and exploitation in the control of PCN. To further investigate the role of SolA in hatching of PCN, the establishment of a highly sensitive analytical method is a prerequisite. Here we present the efficient single-step extraction and UHPLC-MS/MS based analysis for rapid determination of SolA in sub-nanomolar concentrations in tomato root exudate. This method was used to analyze SolA production in different tomato cultivars and related solanaceous species, including the trap crop Solanum sisymbriifolium. Hatching assays with PCN, Globodera pallida, with root exudates of tomato genotypes revealed a significant positive correlation between SolA concentration and hatching activity. Our results demonstrate that there is natural variation in SolA production within solanaceous species and that this has an effect on PCN hatching. The analytical method we have developed can potentially be used to support breeding for crop genotypes that induce less hatching and may therefore display reduced infection by PCN.

scholarly journals Highly Sensitive and Ecologically Sustainable Reversed-Phase HPTLC Method for the Determination of Hydroquinone in Commercial Whitening Creams

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1631
Author(s):  
Mohammed H. Alqarni ◽  
Prawez Alam ◽  
Faiyaz Shakeel ◽  
Ahmed I. Foudah ◽  
Sultan Alshehri
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Solvent System ◽  
Reversed Phase ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Ecologically Sustainable ◽  
Layer Chromatography ◽  
Hptlc Method

Hydroquinone (HDQ) is a natural depigmenting agent, which is commonly used in skin-toning preparations. The safety and greenness of analytical methods of HDQ quantification were not considered in previous literature. Therefore, a highly sensitive and ecologically greener reversed-phase high-performance thin-layer chromatography (RP-HPTLC)-based assay was established for HDQ estimation in four different commercial whitening creams (CWCs). The binary ethanol–water (60:40, v·v−1) mixture was utilized as the green solvent system. The estimation of HDQ was carried out at 291 nm. The present RP-HPTLC-based assay was linear in the 20–2400 ng band−1 range. The present analytical method was highly sensitive based on the detection and quantification data. The other validation parameters, such as accuracy, precision, and robustness, were also suitable for the determination of HDQ. Maximum HDQ quantities were obtained in CWC A (1.23% w·w−1) followed by CWC C (0.81% w·w−1), CWC D (0.43% w·w−1), and CWC B (0.37% w·w−1). The analytical GREEnness (AGREE) score for the present analytical method was estimated as 0.91, indicating the excellent greener characteristics of the present RP-HPTLC assay. These results suggest that the present analytical method is highly sensitive and ecologically sustainable for the quantitation of HDQ in its commercial formulations.

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