Initial acytokinesis during leaf protoplast culture of dihaploid and tetraploidSolanum tuberosum and diploidS. bulbocastanum

1994 ◽  
Vol 37 (4) ◽  
pp. 413-421 ◽  
Author(s):  
W. J. Van Everdink ◽  
L. P. Pijnacker
Keyword(s):  
Protoplast Culture ◽  
Leaf Protoplast

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

Protoplast culture and plant regeneration from Brassica carinata Braun

1987 ◽  
Vol 6 (1) ◽  
pp. 67-69 ◽  
Author(s):  
Phan V. Chuong ◽  
K. P. Pauls ◽  
W. D. Beversdorf
Keyword(s):  
Plant Regeneration ◽  
Protoplast Culture ◽  
Brassica Carinata ◽  
Brassica Carinata Braun

INNOVATIVE APPROACHES TO GENETICS IN AGRICULTURE

1975 ◽  
Vol 17 (4) ◽  
pp. 517-524 ◽  
Author(s):  
F. B. Holl
Keyword(s):  
Protoplast Culture ◽  
Arable Land ◽  
Breeding Systems ◽  
Tissue Cell ◽  
World Population ◽  
Gene Pools ◽  
Land Resources ◽  
Rigorous Evaluation ◽  
Plant Breeding Systems ◽  
Established Plant

The problems of feeding a rapidly expanding world population with decreasing arable land resources are placing increasing demands on our genetic resources and the plant breeders who exploit them. Innovative or novel genetic approaches may provide solutions to some of the problems of genetic analysis and the development of additional genetic variability in plants. The present status of techniques such as wide crossing, DNA feeding, tissue, cell and protoplast culture and somatic cell hybridisation is discussed. Plant tissue culture procedures may enable us to expand the gene pools for disease and pest resistance, greater tolerance to environmental stress, increased quality or possibly to develop new plant types. However, the utility of innovative procedures will require a rigorous evaluation of their potential and limitations, and the ability to produce material which can be readily introgressed into established plant breeding systems.

Protoplast culture

2020 ◽  
pp. 95-120
Author(s):  
D. E. Evans ◽  
J.O.D. Coleman ◽  
A. Kearns
Keyword(s):  
Protoplast Culture
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