Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

2020 ◽  
Vol 33 (4) ◽  
pp. 690-702
Author(s):  
Chellappan Soundar Raju ◽  
Abubakker Aslam ◽  
Devarajan Thangadurai ◽  
Jeyabalan Sangeetha ◽  
Krishnan Kathiravan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Direct Somatic Embryogenesis

scholarly journals Isolation and Characterization of High-Efficiency Rhizobia From Western Kenya Nodulating With Common Bean

2021 ◽  
Vol 12 ◽  
Author(s):  
Clabe Simiyu Wekesa ◽  
Alexandra C. U. Furch ◽  
Ralf Oelmüller
Keyword(s):  
Acetic Acid ◽  
Common Bean ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Rhizobium Tropici ◽  
Western Kenya ◽  
Isolation And Characterization ◽  
The Media ◽  
Different Strains ◽  
Genome Analyses

Common bean is one of the primary protein sources in third-world countries. They form nodules with nitrogen-fixing rhizobia, which have to be adapted to the local soils. Commercial rhizobial strains such as Rhizobium tropici CIAT899 are often used in agriculture. However, this strain failed to significantly increase the common bean yield in many places, including Kenya, due to the local soils’ low pH. We isolated two indigenous rhizobial strains from the nodules of common bean from two fields in Western Kenya that have never been exposed to commercial inocula. We then determined their ability to fix nitrogen in common beans, solubilize phosphorus, and produce indole acetic acid. In greenhouse experiments, common bean plants inoculated with two isolates, B3 and S2 in sterile vermiculite, performed better than those inoculated with CIAT899 or plants grown with nitrogen fertilizer alone. In contrast to CIAT899, both isolates grew in the media with pH 4.8. Furthermore, isolate B3 had higher phosphate solubilization ability and produced more indole acetic acid than the other two rhizobia. Genome analyses revealed that B3 and S2 are different strains of Rhizobium phaseoli. We recommend fieldwork studies in Kenyan soils to test the efficacy of the two isolates in the natural environment in an effort to produce inoculants specific for these soils.

scholarly journals Effects of different combinations of benzyl adenine and indole acetic acid concentrations on in vitro plant regeneration in hexaploid wheat

2012 ◽  
Vol 3 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Ashraf H. Fahmy ◽  
Jiarui Li ◽  
Walaa Abou El-Wafa ◽  
Soheir El-Saeed El-Khodary ◽  
Osama M. El Shihy
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Hexaploid Wheat ◽  
Indole Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Benzyl Adenine ◽  
In Vitro Plant

scholarly journals Production of Stilbenes in Callus Cultures of the Maltese Indigenous Grapevine Variety, Ġellewża

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2112 ◽  
Author(s):  
Mariella Bonello ◽  
Uroš Gašić ◽  
Živoslav Tešić ◽  
Everaldo Attard
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Indole Acetic Acid ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
The Other ◽  
Grapevine Variety

The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Ġellewża. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From results obtained, it was observed that the best callus production was obtained by auxin-enriched MSm. In fact, indole acetic acid and indole acetic acid /6-benzyl aminopurine enhanced biomass accumulation (3.04 g and 3.39 g) as opposed to the others (<1.97 g). On the other hand, parameters showing the presence of flavonoids (tonality, 3.80), particularly anthocyanins (24.09 mg/kg) and total polyphenols (1.42 mg/g), were optimum in the presence of cytokinins, particularly 6-benzyl aminopurine. Analysis for single polyphenols revealed a high amount a particular stilbene: polydatin (glucoside of resveratrol). Resveratrol and other typical polyphenols, found in mature berries, were also found in significant quantities, while the other polyphenolic compounds were found in minimal quantities. This is the first study to describe the production and composition of polyphenols in Ġellewża callus cultures. From the results obtained, it can be seen that this grape tissue is an excellent alternative for the production of polyphenols from the stilbene group, which can be upscaled and exploited commercially.

Sign in / Sign up

Export Citation Format

Share Document