Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and corrleations among them

1992 ◽  
Vol 31 (2) ◽  
pp. 95-103 ◽  
Author(s):  
Thomas E. Taylor ◽  
Richard E. Veilleux
Keyword(s):  
Anther Culture ◽  
Protoplast Culture ◽  
Leaf Disc ◽  
Solanum Phureja ◽  
Disc Regeneration

scholarly journals Leaf Disc Regeneration of Passion Fruit

2011 ◽  
Vol 1 (2) ◽  
Author(s):  
NO Amugune ◽  
HNB Gopalan ◽  
B Bytebier
Keyword(s):  
Leaf Disc ◽  
Passion Fruit ◽  
Disc Regeneration

Anther culture of a 2n pollen producing clone of Solanum phureja Juz. &Buk.

1985 ◽  
Vol 27 (5) ◽  
pp. 559-564 ◽  
Author(s):  
R. E. Veilleux ◽  
J. Booze-Daniels ◽  
E. Pehu
Keyword(s):  
Anther Culture ◽  
Polyacrylamide Gel Electrophoresis ◽  
Pollen Grains ◽  
Unreduced Gametes ◽  
Solanum Phureja ◽  
2N Pollen ◽  
Regenerated Plants ◽  
Series Of Experiments ◽  
Pollen Formation ◽  
First And Second Generation

In a series of experiments, a total of 95 plants were regenerated from culture of 1416 anthers of a single genotype (PP5) of Solanum phureja that expressed a variable frequency of 2n pollen by the genetic equivalent of first division restitution. The regenerated plants included 29 monoploids (2n = x = 12), 58 diploids (2n = 2x = 24) and 8 tetraploids (2n = 4x = 48). Monoploids carrying the potential for 2n pollen formation are central to the development of a breeding scheme to construct highly heterozygous diplandroids. Segregation of tuber flesh color and tuber protein bands revealed by polyacrylamide gel electrophoresis in first and second generation anther-derived diploid plants provided evidence for both embryogenesis of 2n pollen as well as doubling of monoploid genomes as sources of anther-derived diploids. Because of variation for the same genetic markers in anther-derived tetraploids, sources other than embryogenesis of doubly restituted (4n) pollen grains were implied. An enhanced response to anther culture was noted in some anther-derived dihaploids.Key words: unreduced gametes, potato, anther culture, diplandroid, monoploid.

scholarly journals Environmental, genotypic, and ploidy effects on endopolyploidization within a genotype of Solanum phureja and its derivatives

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 506-510 ◽  
Author(s):  
H. R. Owen ◽  
R. E. Veilleux ◽  
D. Levy ◽  
D. L. Ochs
Keyword(s):  
Callus Culture ◽  
Dna Content ◽  
Nuclear Dna ◽  
Protoplast Culture ◽  
Nuclear Dna Content ◽  
Solanum Phureja ◽  
Time Variability ◽  
Flow Cytometric

Flow-cytometric analyses of DNA content were performed on chopped in vivo, in vitro, and protoplast-derived in vitro nuclei of Solanum phureja. An anther-derived monoploid genotype and a diploid and tetraploid clone, derived from callus culture of the monoploid genotype, were used to characterize the influence of in vivo and in vitro environment and explant ploidy level on the extent of endopolyploidization. In addition, protoplast-derived nuclei, from nine anther-derived monoploid genotypes, were examined for genotypic influences on endopolyploidization. DNA distributions of the anther-derived monoploid and callus-derived clones in vivo contained peaks corresponding to 1C, 2C, and 4C DNA levels. By comparison, diploid and tetraploid clones cultured in vitro did not contain 1C DNA peaks. Nuclear DNA content beyond the 4C level was not observed in any of the samples tested. The frequency of monoploid nuclei did not vary significantly among protoplast-derived nuclei from the monoploid genotypes; however, significant differences were detected between replications over time. Variability among the monoploid genotypes was shown for frequency of endoreplicated (4C) nuclei, indicating a genotypic influence on monoploid stability.Key words: potato, monoploid, anther culture, callus culture, protoplast culture, flow cytometry.

Some influence of the hormonal components in nutrient medium onthe induction of novel structures when obtaining rapeseed haploids through in vitro culture

2018 ◽  
pp. 30-37
Keyword(s):  
Anther Culture ◽  
Hormonal Regulation ◽  
Control Plant ◽  
Pollen Grains ◽  
Control Treatment ◽  
Basic Medium ◽  
Nutrient Media ◽  
Oilseed Crops ◽  
Wide Range

Growth regulators, phytohormones, both natural and artificial, are the main means to control plant ontogenesis. They are involved in regulating the processes of cell differentiation and cell divisions, the formation of tissues and organs, the changes in the rate of growth and development, the duration of the certain stages of ontogenesis. The main classes of phytohormones used in plant biotechnology, in particular, in the induction of haploid structures, are auxins and cytokinins. The mechanism of action of phytohormones on a cell is rather complicated and may have a different character. Understanding the characteristics of the action of phytohormones is complicated by the fact that the system of hormonal regulation of plant life is multicomponent. This is manifested in the fact that the same physiological process is most often influenced not by one, but by several phytohormones, covering a wide range of aspects of cell metabolism. In connection with the foregoing, the purpose of our work was to test a set of nutrient media with different basic composition and different proportions of phytohormones to determine the patterns of their influence on the processes of haploid structure induction in rape anther culture using accessions, developed at the Institute of Oilseed Crops NAAS. The material used was two accessions of winter rapeseed (No. 1 and No. 2) and one sample of spring rapeseed, provided by the Rapeseed Breeding laboratory of the Institute of Oilseed Crops. Incised inflorescences were kept against the background of low temperature of 6–8 ° C for several days, and then, under aseptic conditions, anthers with unripe pollen grains were isolated and planted on nutrient media differing in both basic mineral composition and content of phytohormones. MS (Murashige & Skoog 1962) and B5 (Gamborg et al 1968) media were used as basic media. Phytohormones were added to the basic media in various combinations – BA, 2,4-D, NAA at the concentrations of 0.1-0.6 mg/l. In each treatment up to 300 anthers were cultivated. Differences between treatments were evaluated using standard t-test. Studies have shown that in the anther culture of rapeseed on the tested nutrient media, morphogenic structures of different types (embryoids and callus) were originated. Synthetic auxin 2,4-D, regardless of the composition of the basic medium, caused the formation of structures of both types, though with a low frequency. Phytohormone BA of the cytokinin type had a similar effect. In this case, the frequency of structures was slightly higher, and the developed structures were represented mainly by embryoids. The joint action of cytokinin and auxin was the most favorable for the initiation of morphogenic structures. Such combination of phytohormones caused the formation of these structures with a frequency of 24.5-14.7% in the studied genotypes of winter rape. A similar effect of phytohormones on the induction and development of morphogenic structures was also observed in spring rape. In this case, a single basic MS medium was used. The experiment included treatments where phytohormones were absent (control), as well as various combinations of auxin and cytokinin. In the control treatment, the formation of new structures was not noted. In treatments with phytohormones, in addition to the medium with the combination of auxin and cytokinin, the medium in which only cytokinin was present was also rather effective. The treatment in which the action of auxin 2,4-D was combined with the action of another auxin, NAA, turned out to be practically ineffective. Thus, it was found that for the induction of morphogenic structures from microspores in rape anther culture of the tested genotypes, the combination of cytokinin with auxin, or the use of only single cytokinin BA without other phytohormones, had the most positive effect.

Effect of a Modified Potato Medium on Anther Culture of Wheat 1

Crop Science ◽  
1987 ◽  
Vol 27 (2) ◽  
pp. 351-354 ◽  
Author(s):  
J. E. Marburger ◽  
D. J. Sammons ◽  
G. W. Schaeffer
Keyword(s):  
Anther Culture

Возрастные и органоспецифичные различия метаболома растений картофеля Solanum phureja

2018 ◽  
Vol 65 (6) ◽  
pp. 451-462
Author(s):  
Р. К. Пузанский ◽  
В. В. Емельянов ◽  
А. Л. Шаварда ◽  
Т. А. Гавриленко ◽  
М. Ф. Шишова
Keyword(s):  
Solanum Phureja
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